Swimming and feeding in Periphylla periphylla (Scyphozoa, Coronatae)
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Some western Norwegian fjords host extraordinarily abundant and persistent populations of the mesopelagic, coronate scyphomedusa, Periphylla periphylla. In these environments, from late autumn to spring, the medusae undertake regular diel vertical migrations into surface waters. From unique observations obtained with a remotely operated vehicle (ROV), including observations made without artificial light, we observed that 90% of the medusae swam with their tentacles in aboral position. Stomach content analyses of surface-collected specimens revealed that the medusae ate mainly calanoid copepods, but ostracods and large euphausiids were also prominent components of their diets. The clearance rate potential of P. periphylla, assessed from in situ observations and stomach contents, was comparable to that of similar-sized, epipelagic gelatinous species. Our findings suggest that P. periphylla behave as active predators in surface waters.
KeywordsStomach Content Diel Vertical Migration Remotely Operate Vehicle Dome Height Gelatinous Zooplankton
Planktonic cnidarians comprise a heterogeneous group in terms of functional morphology, swimming and foraging patterns. The various mechanisms involved in prey encounter and capture processes can lead to interspecific dietary differences (Costello and Colin 2002). Our current knowledge of these mechanisms is fragmentary. As a consequence, so is our understanding of the prey selection patterns and trophic impacts of cnidarians. Based on their prey encounter tactics, gelatinous planktivores have been classified as either ambushing or cruising entangling predators (Greene 1985). Ambushing predators remain stationary, waiting for prey to contact them, and only move to reposition. Cruising predators are in constant motion and swimming and prey capture are simultaneous, interrelated processes (Madin 1988). Recent studies have proposed that predation mode is connected to bell morphology for some shallow-water hydromedusae and scyphomedusae (Ford et al. 1997; Colin and Costello 2002; Colin et al. 2003), ambushers being characterized by streamlined bells and cruisers by flat bells. However, alternative foraging strategies seem to exist (Raskoff 2002), suggesting that a refinement of the above classification scheme is needed.
Our understanding of the abundance, distribution and natural behaviours of meso- and bathypelagic gelatinous zooplankton is in its infancy (Dennis 2003). Traditional approaches, such as sampling with plankton nets, tend to damage their delicate bodies (Dennis 2003; Haddock 2004). The creatures from this deep, dim realm may be sensitive to the changes in light, pressure and temperature often associated with bringing live samples to the surface. And so, retrieving specimens in pristine condition for shipboard and laboratory experiments remains immensely challenging. The introduction of research submarines has permitted detailed explorations of deep-sea animals in their natural habitats (Haddock 2004; Robison 2004). In situ observations and experimentation at great depth have become realistic, albeit expensive, alternatives to laboratory studies (NRC 2003). Several challenges, such as controlling variables and replicating observations (Madin 1988), may nonetheless pertain to in situ operations. Semi-enclosed habitats, like fjords, provide ideal environments to study midwater residents, such as some gelatinous zooplankton. Water exchange is generally restricted, especially in fjord basins, and the animal communities therein remain resident for prolonged periods. The inherently calm sea state in fjords permits continuous operations, virtually undisturbed by rough weather.
Dense populations of the perennial, midwater coronate scyphomedusa Periphylla periphylla have been reported for several western Norwegian fjords (Fosså 1992; Youngbluth and Båmstedt 2001). In Lurefjorden, P. periphylla is an apex predator, with no obvious competitors or predators (Youngbluth and Båmstedt 2001). The majority of the population undertakes diel vertical migrations, with distances ranging from 100 to 400 m, and nocturnal surface swarming has been occasionally observed from late autumn to spring (Youngbluth and Båmstedt 2001). Preliminary observations have indicated that P. periphylla drift with their tentacles first or laterally extended. The former posture was recently described for the midwater narcomedusae Solmissus spp. and termed “ramming” (Raskoff 2002). Ramming involves approaching your prey tentacles-first, to avoid the fluid disturbance associated with swimming-induced escape responses.
Ramming may be a common foraging strategy for midwater medusae, but our knowledge on the diets and predation potentials of “rammers” is scarce (Raskoff 2002). We obtained video recordings of P. periphylla, using a remotely operated vehicle (ROV), as the population surfaced in Lurefjorden, April 2004. We also assessed the abundance and distribution of the medusae and their mesozooplankton prey, and obtained stomach contents from surface-collected specimens. Their potential clearance rates were quantified using both field observations and stomach contents.
Materials and methods
Sampling and all observations were made from 20 to 26 April 2004 in Lurefjorden, western Norway. The fjord is 22 km long and its maximum depth is 439 m. Water exchange is mainly driven by tidal currents and local weather conditions (Youngbluth and Båmstedt 2001). Vertical profiles of salinity, temperature and dissolved oxygen were obtained using a Seabird 911 CTD (Sea-Bird Electronics, Washington, USA).
P. periphylla: behaviour and swimming performance
The behaviour of P. periphylla was observed in situ using the ROV Aglantha. The vehicle was equipped with a Sony EVI-401 colour-camera, and the observations were recorded on SVHS and DVCam tapes for later analysis. During the study period, we made four dives videotaping medusae at 15–40 m depth. The dives took place at dusk and dawn, when large specimens (>5 cm in coronal diameter) appeared close to the surface. On two of the dives, from hereon referred to as “behavioural dives”, we tracked single medusae (∼3–8 min per medusa, n = 15) using exclusively red illumination (>660 nm). On the two remaining dives, Aglantha maintained a fixed position at a depth of 25–30 m. The lights were switched off, the camera tilted upwards and P. periphylla videotaped as they swam by the camera at a distance. Using this setup, lighting and mechanical disturbance from the vehicle were unlikely to affect medusan behaviour, but the observation period was reduced to 15–155 s per medusa (n = 102). The natural sunlight and moonlight proved sufficient to observe both bell contraction rates (s−1) and tentacle postures. For the medusae videotaped under red illumination, we also noted whenever a tentacle was inserted into the gastric cavity (apparent feeding bout) and the total time that each tentacle was concealed (apparent handling time, s). These observations were used to estimate clearance rates of the medusae (see below). Unfortunately, the video records lacked sufficient resolution to identify prey attached to the tentacles.
P. periphylla: vertical distributions, stomach contents and prey abundance
The vertical distributions of P. periphylla were determined using Aglantha (n = 4 dives). We profiled the whole water column before and after the “behavioural dives” and noted the depth of each medusa. The descent rate of the vehicle was <0.5 m s−1. Four 500 W halogen lights and four 150 W high intensity discharge (HID) gas-arc lights illuminated the field of view. From these profiles, we assessed to what extent the medusae overlapped with their mesozooplankton prey. P. periphylla (n = 70) were also collected from the surface waters at night, over the entire 7-day period, using 3–10 l containers. Their stomachs were opened and the contents immediately examined at 10–50× magnification with a light microscope.
To determine the abundance and vertical distribution of mesozooplankton, Multinet samples (0.25 m2 mouth area, 180 μm mesh, Hydrobios Company, n = 2 hauls) were collected from bottom to surface after the “behavioural dives”. The five nets were opened and closed to cover the intervals 400–300, 300–200, 200–100, 100–50 and 50–0 m depth, respectively. The samples were immediately preserved in 5% buffered formalin and returned to the laboratory for species identification and enumeration.
P. periphylla: encounter zone volumes and tentacle densities
The encounter radii of gelatinous tentaculate predators are determined by the arrangement and dimensions of tentacles and their swimming behaviours. In constructing a conceptual encounter model for gelatinous predators, Madin (1988) introduced the two spatial parameters “encounter zone” and “tentacle density”. He defined the encounter zone as “the space into which tentacles can extend when the predator is fishing” and tentacle density as “the fraction of the encounter zone filled with tentacles”. We expressed these two parameters for P. periphylla, based on the observed behaviour and tentacle postures, to facilitate comparison with other functional types of medusae (Madin 1988).
P. periphylla: clearance rate estimates
P. periphylla: fluid flow
The fluid flow around swimming P. periphylla was visualized using sepia ink. Medium-sized specimens (4–5 cm in coronal diameter, n = 5) were dipped from the surface waters at night and brought back to the onshore laboratory in 5 l dark chambers. The medusae were placed in a planktonkreisel (73 cm in diameter, 15 cm in width) with continuous flow and kept in a dark and temperature-controlled (10°C) room. Single medusae were then transferred to an experimental tank (34 × 34 × 59 cm) for shorter time periods. Here, the ink was introduced ahead of the swimming medusae, which were then videotaped (Sony CCD AVC-D5CE, Olympus Sigma Mini-Wide lens) as they swam through it. All sequences were filmed under red illumination. Attempts were made to use particles as current tracers instead of sepia ink. However, their contrast against the dim (red) background was insufficient to support further analyses. This prevented a more thorough investigation of the important flow characteristics around swimming P. periphylla.
The water column was virtually isothermal (6.0–6.8°C) and isohaline (31.9–33.1 ppt) at the sampling station. From saturation in surface waters, the oxygen content decreased to 2.9 ml l−1 near the bottom, well above hypoxic levels (Diaz and Rosenberg 1995).
P. periphylla: behaviour and swimming performance
There was no significant difference (t test, P > 0.05, df = 115) in pulsation frequency between medusae viewed under red illumination (11.8 ± 2.6 contractions min−1, mean ± SD, n = 15) and natural illumination (13.0 ± 3.2 contractions min−1, mean ± SD, n = 102). We also did not observe changes in swimming direction or tentacle deployment patterns, suggesting that the medusae were unaffected by the red light.
Eleven out of the 15 medusae observed under red illumination were apparently feeding when swimming with their tentacles first. Whenever a tentacle arched away from the dome and approached the mouth, the distal part coiled. The coiled portion was inserted into the mouth and concealed in the gastric cavity. This concealment lasted for 52.0 ± 17.8 s (mean ± SD, n = 15).
P. periphylla: vertical distributions, prey abundance and stomach contents
The main prey items found in the stomachs of P. periphylla were calanoid copepods, especially Calanus spp. 92.9% of the medusae contained copepods (range 1–131 copepods per medusa, mean ± SD = 23.7 ± 29.8), 15.7% contained exoskeletons of the northern krill Meganyctiphanes norvegica (range 1–3 krill per medusa, mean ± SD = 1.3 ± 0.7) and 8.6% contained the ostracod Conchoecia spp. (range 2–13 ostracods per medusa, mean ± SD = 4.5 ± 4.2). The dissected medusae had a mean coronal diameter of 7.5 ± 1.7 cm (mean ± SD, n = 70). A total of 24% of the copepods (total n = 1,538) were partly digested and not identifiable to the genus, which prevented calculations of electivity indices.
P. periphylla: encounter zone volumes, tentacle densities and clearance rates
The mean encounter zone volumes and tentacle densities were 6.9 ± 3.9 × 10−3 m3 and 3,564 ± 1,782 ppm, respectively (mean ± SD, n = 15). From the in situ observations of apparent feeding bouts we estimated a mean clearance rate (a1) of 0.76 ± 0.16 m3 day−1 (mean ± SD, n = 15). This estimate was somewhat higher than that based on the stomach contents of copepods (a2 = 0.31 ± 0.43 × 10−6 m3 s−1, mean ± SD, n = 70). However, the clearance rate estimates based on in situ observations assume 100% retention efficiencies, and are therefore likely to be overestimates of true clearance rates.
P. periphylla: fluid flow
P. periphylla swam tentacles-first in the experimental tank. The flow appeared to be laminar passing through the tentacles, and toroids were first observed when the ink approached the marginal lappets. The wake seemed more consistent with jet propulsion than a rowing kind of thrust production. However, these analyses are preliminary, and more rigorous studies are encouraged to reveal the detailed flow patterns around these medusae.
The prey encounters of ambush predators are exclusively determined by tentacle dimensions and postures, prey size and motility. Prolate bells experience less drag than oblate bells (Colin et al. 2003), and minimizing the area of the velar opening through which water exits the subumbrellar cavity increases the velocity and momentum flux of the expelled fluid (Colin and Costello 2002). This facilitates rapid repositioning, whereafter the medusae can resume their drift posture. Based exclusively on their prolate shape, P. periphylla would be expected to behave as ambushers. However, the medusae observed in this study were constantly swimming. Cruisers generally have a rowing propulsive mode. Their swimming enhances the water flow past tentacles and other primary capture surfaces (Costello 1992), thus facilitating prey encounter. Stealth predation or “ramming” may be considered as an intermediate strategy. Swimming and feeding are interrelated, unlike for “pure” ambush predators. However, the swimming-generated fluid disturbance seems decoupled from prey encounters (Raskoff 2002), unlike for cruising predators.
P. periphylla were most often observed swimming, and feeding, with their tentacles in aboral position, suggesting that they behaved as “ramming” stealth predators. Similar tentacle postures have been described for other coronate scyphomedusae (Larson 1979), some narcomedusae (Madin 1988; Mills and Goy 1988; Larson et al. 1989; Raskoff 2002) and limnomedusae (Raskoff 2002). Fewer specimens were observed swimming with their tentacles laterally extended. One would expect the fluid deformations generated by swimming medusae to be readily perceived by both copepods and euphausiids and elicit pre-encounter escape responses. However, if the swimming-generated fluid disturbance for P. periphylla was decoupled from their encounter zone, as described for other “rammers” (Raskoff 2002), this could enable them to approach highly motile (and sensitive) prey without causing escape responses. The presence of large euphausiids in the stomachs of surface-collected medusae, as well as the “tank observations” of main flow patterns support this notion.
Compared with the species/genera of medusae listed in Madin (1988), and regardless of the encounter zone shape and size, P. periphylla have high tentacle densities. For a given tentacle posture, P. periphylla can increase the volume searched for prey by (1) increasing the dimension and/or number of tentacles, or (2) increasing the swimming speed. Both infer energetic costs that have to be compensated. We would expect medusae not behaving as “rammers” to increase their cone radius (r2), thereby exposing more of the tentacle surface in the direction of movement. And indeed, the less dominant posture (7.7%) observed in this study was laterally extended tentacles, which corresponds to maximizing the projected surface area.
The coronate tentacle has a tube-within-a-tube arrangement and root-like extensions into the umbrellar mesoglea, providing strength and rigidity (Larson 1979). This enables P. periphylla to retain its tentacles in upright position during forward motion. The tentacles were often arched outwards with bent tips. This was most probably a function of tentacle length (Larson et al. 1989), as the tentacles tapered with increasing distance from the bell, reducing their ability to withstand drag. The 12 tentacles were widely spaced, an arrangement seemingly not well suited for capturing small prey items (Madin 1988; Purcell and Mills 1988).
Both the strong tentacles and giant nematocysts of P. periphylla may be a specialization for capturing large and motile prey. Their cnidome consists of holotrichous isorhizas and heterotrichous microbasic euryteles, typical for all coronates (Jarms et al. 2002). The tentacles are lined with euryteles, thought to be responsible for penetrating and holding on to prey. Their giant euryteles are the largest described for any scyphozoan (Jarms et al. 2002). Keeping their tentacles in the mouth for a prolonged period following capture (=long handling time) may decrease the chances of prey escaping from the gut.
Approximately 97% of the surface-collected P. periphylla contained prey. Whether the dominance of calanoid copepods in their stomachs reflected active selection, or merely the predominance of these prey in the plankton, remains uncertain. Both crustaceans and decapods, and midwater fishes have been reported in the stomachs of P. periphylla sampled with plankton nets (Maas 1897; Larson 1979). However, Youngbluth and Båmstedt (2001) suspected that the medusae ingested extraneous prey in the cod-end bags, lending little support to the above records. Other ramming medusae eat mainly large, gelatinous prey (Mills and Goy 1988; Larson et al. 1989; Mills et al. 1996; Raskoff 2002). Apart from P. periphylla, cnidarians and ctenophores are virtually absent in Lurefjorden (Youngbluth and Båmstedt 2001), but whether this is due to predation by P. periphylla is not known. The digestion of potential gelatinous prey would be rapid, making detection from gut contents difficult (e.g., Arai 2005).
The P. periphylla we examined contained much higher numbers of prey than previously reported. In March 1992, Fosså (1992) dissected 39 surface-collected P. periphylla from Lurefjorden. Only 24 of the medusae contained a total of 39 preys. The preys were identified as Calanus finmarchicus and Euchaeta norvegica in a 2:1 ratio, as well as remnants of a natant decapod. In August and December 1999, Youngbluth and Båmstedt (2001) collected 41 medusae from Lurefjorden with ROV samplers. Only 10% contained prey, and in small numbers (1–3 copepods or a single chaetognath and 2–4 ostracods per medusa). Since there was no information on prey densities (Fosså 1992) or depth of capture (Youngbluth and Båmstedt 2001) in these studies, clearance rates for comparison could not be calculated. Our clearance rate estimates for P. periphylla (3.6 and 8.8 × 10−6 m3 s−1) are consistent with published records from similar-sized gelatinous zooplanktivores feeding on small crustaceans (Sørnes and Aksnes 2004).
Meganyctiphanes norvegica can be important food items for P. periphylla in Lurefjorden. The basal metabolic requirements of medium-sized (1.5–7.5 cm in coronal diameter) P. periphylla are equivalent to 0.2–8 mg Carbon day−1 (Youngbluth and Båmstedt 2001). M. norvegica from Skagerrak waters and the Norwegian northwest coast range from 30–80 mg Dry Weight individual−1 (Båmstedt and Karlson 1998). Assuming a mean carbon content of 40.2% of DW (Lindley et al. 1999), each euphausiid contains 12–32 mg C. Based on carbon requirements, one M. norvegica would therefore meet the daily metabolic needs of medium-sized P. periphylla. It would take 1–34 (7.8 ± 0.6, mean ± SD, Youngbluth and Båmstedt 2001) Calanus spp. to meet the same demands.
Surface swarming by medusae normally inhabiting the midwater realm is rare. The unique setting in this study allowed detailed observations of the swimming behaviours and tentacle postures adopted by P. periphylla. Our findings suggest that the medusae behave as active “rammers” in the epipelagic, and not passive drifters as previously assumed. Because P. periphylla are notoriously difficult to keep in captivity (Youngbluth and Båmstedt 2001, personal observations), field studies may be the easiest, and perhaps the only, way to explore their feeding ecology.
This investigation was supported by EUROGEL (EUROpean GELatinous Zooplankton, European Commission Contract no. EVK-CT-2002-00074), the Research Council of Norway (project no. 146994/120), the Meltzer Foundation and the Finnish Cultural Foundation. The assistance of collaborating scientists and the crew of R/V Håkon Mosby are greatly appreciated.
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