Marine Biology

, Volume 153, Issue 3, pp 277–283 | Cite as

Microbial activity and accumulation of organic matter in the burrow of the mud shrimp, Upogebia major (Crustacea: Thalassinidea)

  • K. KinoshitaEmail author
  • M. Wada
  • K. Kogure
  • T. Furota
Research Article


Microbial activity and accumulation of organic matter in the burrow of the thalassinidean mud shrimp, Upogebia major, were studied on a tidal flat along the northern coast of Tokyo Bay, Japan. The burrow of U. major is Y-shaped with an upper U-shaped part plus a lower I-shaped part. Its lower part can extend to a depth of 2 m. In the present study, we compare electron transport system activity (ETSA), bacterial abundance and organic matter content [total organic carbon (TOC), total nitrogen (TN) and chlorophyll a (chl. a)] of the burrow wall sediment with the tidal flat surface and non-burrow sediments. We also compared the U- and I-shaped part in terms of these parameters. ETSA in the burrow wall was higher than at the tidal flat surface in the warmer season, and was always higher than at surrounding non-burrow sediments. Bacterial abundance in the burrow wall was higher than at the tidal flat surface and surrounding sediment. TOC and TN contents in the burrow wall were two to three times higher than those at the tidal flat surface and non-burrow sediments, regardless of season. However, there was no significant difference in chl. a content between burrow wall and tidal flat surface. These results suggest that organic enrichment of the burrow wall is a result of organic matter particles such as phytodebris accumulation along the burrow wall. For all parameters of the burrow walls, no statistical differences were found between the two parts. Present results indicate that U. major actively transports the water containing suspended organic particles not only through the U-part but also into the deeper I-part. These indicate that burrow of the mud shrimp provides a dynamic environment for microbial community in tidal flat sediment.


Total Organic Carbon Organic Matter Content Bacterial Abundance Total Nitrogen Content Electron Transport System Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank the following for their superb cooperation, without which this project and manuscript could never have been realized. Drs. T. Miyajima, H. Urakawa, A. Shibata, R. Fukuda-Sohrin and M. Shimanaga offered useful suggestions on bacteria counting, chl. a and CHN analysis, and sediment sampling. S. Arita assisted in the field observations. Y. Hasuo and S. Hasuo, superintendents of Gyotoku Bird Observatory, provided the opportunity to use the field site. Richard S. Lavin assisted in editing the English manuscript. This study was also supported by Grants-in-Aid for Scientific Research, No. 0856093, and Creative Basic Research, No. 12NP0201 (Dynamics of the Ocean Biosystem, DOBIS), of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The experiments detailed in this study comply with the current laws in Japan.


  1. Allanson BR, Skinner D, Imberger J (1992) Flow in prawn burrows. Estuar Coast Shelf Sci 35:253–266. doi: 10.1016/S0272-7714(05)80047-2 CrossRefGoogle Scholar
  2. Aller RC, Yingst YJ, Ullman WJ (1983) Comparative biogeochemistry of water in intertidal Onuphis (polychaeta) and Upogebia (crustacea) burrows: temporal patterns and causes. J Mar Res 41:571–604CrossRefGoogle Scholar
  3. Astall CM, Taylor AC, Atkinson RJA (1997) Behavioural and physiological implication of a burrow-dwelling lifestyle for two species of upogebiid mud-shrimp (Crustacea: Thalassinidea). Estuar Coast Shelf Sci 44:155–168. doi: 10.1006/ecss.1996.0207 CrossRefGoogle Scholar
  4. Bird FL, Boon PI, Nichols PD (2000) Physicochemical and microbial properties of burrows of the deposit-feeding thalassinidean ghost shrimp Biffarius arenosus (Decapoda: Callianassidae). Estuar Coast Shelf Sci 51:279–291. doi: 10.1006/ecss.2000.0676 CrossRefGoogle Scholar
  5. Broberg A (1985) A modified method for studies of electron transport system activity in freshwater sediments. Hydrobiologia 120:181–187. doi: 10.1007/BF00032140 CrossRefGoogle Scholar
  6. Cadée GC (2001) Sediment dynamics by bioturbating organisms. In: Reise K (ed) Ecological comparisons of sedimentary shores, ecological studies, vol 151. Springer, Berlin, pp 127–148CrossRefGoogle Scholar
  7. Candisani LC, Sumida PYG, Pires-Vanin AMS (2001) Burrow morphology and mating behavior of the thalassinidean shrimp Upogebia noronhensis. J Mar Biol Ass UK 81:799–803. doi: 10.1017/S0025315401004611 CrossRefGoogle Scholar
  8. Coelho VR, Cooper RA, Rodrigues SA (2000) Burrow morphology and behavior of the mud shrimp Upogebia omissa (Decapoda: Thalassinidea: Upogebiidae). Mar Ecol Prog Ser 200:229–240CrossRefGoogle Scholar
  9. Dobbs FC, Guckert JB (1988) Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow. Mar Ecol Prog Ser 45:69–79CrossRefGoogle Scholar
  10. Dworschak PC (1981) The pumping rates of the burrowing shrimp Upogebia pusilla (Petagna) (Decapoda: Thalassinidea). J Exp Mar Biol Ecol 52:25–35. doi: 10.1016/0022-0981(81)90168-4 CrossRefGoogle Scholar
  11. Dworschak PC (1983) The biology of Upogebia pusilla (Petagna) (Decapoda, Thalassinidea): I. The burrows. PSZNI Mar Ecol 4:19–43CrossRefGoogle Scholar
  12. Felder DL, Griffis RB (1994) Dominant infaunal communities at risk in shoreline habitats: burrowing thalassinid Crustacea. (OCS Study # MMS 94-0007). US Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Regional Office. New Orleans, Louisiana, pp 87Google Scholar
  13. Griffen BD, DeWitt TH, Langdon C (2004) Particle removal rates by the mud shrimp Upogebia pugettensis, its burrow, and a commensal clam: effects on estuarine phytoplankton abundance. Mar Ecol Prog Ser 269:223–236CrossRefGoogle Scholar
  14. Griffis RB, Suchanek TH (1991) A model of burrow architecture and trophic modes in thalassinidean shrimp (Decapoda: Thalassinidea). Mar Ecol Prog Ser 79:171–183CrossRefGoogle Scholar
  15. Kenner RA, Ahmed SI (1975) Measurements of electron transport activities in marine phytoplankton. Mar Biol 33:119–127. doi: 10.1007/BF00390716 CrossRefGoogle Scholar
  16. Kinoshita K (2002) Burrow structure of the mud shrimp Upogebia major (Decapoda: Thalassinidea: Upogebiidae). J Crust Biol 22:474–480CrossRefGoogle Scholar
  17. Kinoshita K, Itani G (2005) Interspecific differences in the burrow morphology between the sympatric mud shrimps, Austinogebia narutensis and Upogebia issaeffi (Crustacea: Thalassinidea: Upogebiidae). J Mar Biol Ass UK 85:943–947. doi: 10.1017/S0025315405011902 CrossRefGoogle Scholar
  18. Kinoshita K, Wada M, Kogure K, Furota T (2003a) Mud shrimp burrows as dynamic traps and processors of tidal-flat materials. Mar Ecol Prog Ser 247:159–164CrossRefGoogle Scholar
  19. Kinoshita K, Nakayama S, Furota T (2003b) Life cycle characteristics of the deep-burrowing mud shrimp Upogebia major (Thalassinidea: Upogebiidae) on a tidal flat along the northern coast of Tokyo Bay. J Crust Biol 23:318–327CrossRefGoogle Scholar
  20. Koike I, Mukai H (1983) Oxygen and inorganic nitrogen contents and fluxes in burrows of the shrimps Callianassa japonica and Upogebia major. Mar Ecol Prog Ser 12:185–190CrossRefGoogle Scholar
  21. Kristensen E, Jensen MH, Aller RC (1991) Direct measurement of dissolved inorganic nitrogen exchange and denitrification in individual polychaete (Nereis virens) burrows. J Mar Res 49:355–377CrossRefGoogle Scholar
  22. Meysman FJR, Middelburga JJ, Heip CHR (2006) Bioturbation: a fresh look at Darwin’s last idea. Trends Ecol Evol 21:688–695CrossRefGoogle Scholar
  23. Middelburg JJ, Klaver G, Nieuwenhuize J, Wielemaker A, de Haas W, Vlug T, van der Nat JFWA (1996) Organic matter mineralization in intertidal sediments along an estuarine gradient. Mar Ecol Prog Ser 132:157–168CrossRefGoogle Scholar
  24. Nickell LA, Atkinson RJA (1995) Functional morphology of burrows and trophic modes of three thalassinidean shrimp species, and a new approach to the classification of thalassinidean burrow morphology. Mar Ecol Prog Ser 128:181–197CrossRefGoogle Scholar
  25. Packard TT (1985) Measurement of electron transport activity of microplankton. In: Jannasch HW, Williams PJL (eds) Advances in aquatic microbiology, vol 3. Academic, London, pp 207–261Google Scholar
  26. Papaspyrou S, Gregersen T, Cox R, Thessalou-Legaki M, Kristensen E (2005) Sediment properties and bacterial community in burrows of the ghost shrimp Pestarella tyrrhena (decapoda: Thalassinidea). Aquat Microb Ecol 38:181–190CrossRefGoogle Scholar
  27. Papaspyrou S, Gregersen T, Kristensen E, Christensen B, Cox RP (2006) Microbial reaction rates and bacterial communities in sediment surrounding burrows of two nereidid polychaetes (Nereis diversicolor and N. virens). Mar Biol 148:541–550. doi: 10.1007/s00227-005-0105-3 CrossRefGoogle Scholar
  28. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
  29. Posey MH, Dumbauld BR, Armstrong DA (1991) Effects of a burrowing mud shrimp, Upogebia pugettensis (Dana), on abundances of macro-Infauna. J Exp Mar Biol Ecol 148:283–294. doi: 10.1016/0022-0981(91)90088-E CrossRefGoogle Scholar
  30. Vaugelas Jd, Buscail R (1990) Organic matter distribution in burrow of the thalassinia crustacean Callichirus laurae, Gulf of Aqaba (Red Sea). Hydrobiologia 207:269–277. doi: 10.1007/BF00041465 CrossRefGoogle Scholar
  31. Vogel S, Bretz WL (1971) Interfical organisms: passive ventilation in the velocity gradients near surfaces. Science 175:210–211. doi: 10.1126/science.175.4018.210 CrossRefGoogle Scholar
  32. Westrich JT, Berner RA (1984) The role of sedimentary organic matter in bacterial sulfate reduction: the G-model tested. Limnol Oceanogr 29:236–249CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Faculty of Environmental and Symbiotic SciencesPrefectural University of KumamotoKumamotoJapan
  2. 2.Ocean Research InstituteThe University of TokyoTokyoJapan
  3. 3.Department of Environmental Science, Faculty of ScienceToho UniversityChibaJapan

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