Physiological and immunological conditions of wild populations of Farfantepenaeus duorarum from the campeche sound (Crustacea, Penaeidae)
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To assess the health status of aquatic ecosystems, specialized tools and strategies are needed to study the changes induced in oceanic systems by human activity. The Gulf of Mexico has a tremendous ecological importance because of its biological diversity. The present paper defines baseline levels of blood metabolites and immune components for wild tropical populations of F. duorarum: an important shrimp species of the Gulf of Mexico. Osmotic pressure (OP), osmotic capability (OC), oxy hemocyanin (OxyHc), glucose, digestive gland glycogen, proteins, cholesterol, lactate, acylglycerols (AG) were used as indicators of physiological and nutritional status; phenoloxidase activity, quantification of hemocytes, as well as respiratory burst, were used to assess the immunological status. There were no differences between sexes in either live weight or physiological and nutritional conditions. Only total blood protein, oxy hemocyanin, and digestive gland glycogen showed normally distributed data. All the remaining blood parameters were right- or left-skewed. A median live weight of 9.5 g was registered for the sampled population. Median values of 955 and −91.85 mOsm/kg (OP and OC, respectively) were obtained, indicating that shrimp were slightly hypo-osmotic in relation to sea water. A proportion of 27% of OxyHc of the total protein (OxyHc/Prot) was calculated for the F. duorarum population. ProPO and PO showed no normal distribution and were not different between sexes. A right-skewed distribution was observed. Total hemocytes, hyaline (HC) and granular cells (GC) showed differences between sexes, with higher values in females than males. A similar proportion of HC (69%) and GC (30%) of total cells were observed between sexes. Basal and activated respiratory burst showed differences between sexes with higher values for females than for males. The current results provide indicators of the nutritional and immune status of F. duorarum that can be used to detect changes in trophic relations and health at a population level.
KeywordsOsmotic Pressure Digestive Gland Respiratory Burst Hemocyte Sperm Quality
This paper was partially financed by UC-MEXUS program to C. Rosas and E. L. Cooper. We thank Tomas Jesús García for L. setiferus sampling and the personnel of MARSEP II for the F. duorarum sampling.
- Balcom B (1994) Dispersed oil toxicity tests with species indigenous to the Gulf of Mexico. Rep MMS: 14-35-0001-30617, Minerals Management Service, pp 1–8Google Scholar
- Chen JC, Cheng S-Y (1993b) Studies on hemocyanin and hemolymph protein levels of Penaeus japonicus based on sex, size and moulting cycle. Comp Biochem Physiol 106B:293–296Google Scholar
- Drach P, Tchernigovtzeff C (1967) Sur le méthod du détrmination des stades d´intermue et son application générale aux crustacés. Biol Mar 8:595–610Google Scholar
- Fielder DR, Rao KR, Fingerman M (1971) A female-limited lipoprotein and diversity of hemocyanin components in the dimorphic variants of the fiddler crab, Uca pugilator. Comp Biochem Physiol 39B:291–297Google Scholar
- Hernández-López J, Gollas-Galván T, Vargas-Albores F (1996) Activation of the prophenoloxidase system of brown shrimp (Penaues californiensis Holmes). Comp Biochem Physiol 113C(1):61–66Google Scholar
- Le Moullac G, Le Groumellec ML, Ansquer D, Froissard S, Levy P, Aquacop (1997) Heamatological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis. Fish Shellfish Immunol 7:227–234Google Scholar
- Pascual C, Sánchez A, Zenteno E, Cuzon G, Gaxiola G, Brito R, Gelabert R, Hidalgo E, Rosas C (2006) Biochemical, physiological, and immunological changes during starvation in juveniles of Litopenaeus vannamei. Aquaculture Press, corrected proof, available online 10 August 2005Google Scholar
- Teshima S (1997) Phospholipids and sterols. In: D´Abramo L, Conklin DE, Akiyama DM (eds) Crustacean nutrition. The World Aquaculture Society, Baton Rouge, pp 85–107Google Scholar
- Zar JH (1974) Bioestatistical analysis. Prentice Hall, Englewood CliffGoogle Scholar