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Marine Biology

, Volume 151, Issue 3, pp 1051–1058 | Cite as

Effects of salinity on the clam Chamelea gallina. Part I: alterations in immune responses

  • Valerio Matozzo
  • Marta Monari
  • Jurgen Foschi
  • Gian Paolo Serrazanetti
  • Otello Cattani
  • Maria Gabriella Marin
Research Article

Abstract

In the present study, the effects of differing salinities on some important functional responses of haemocytes from the clam, Chamelea gallina, were investigated. The animals were kept for 7 days at 28‰ (hyposalinity), 34‰ (control) and 40‰ salinity (hypersalinity), and total haemocyte count (THC), haemocyte volume, phagocytosis, lysozyme-like activity (in both haemocyte lysate and cell-free haemolymph) were measured. The survival-in-air test was also performed. Clams kept at 28‰ showed significantly increased THC with respect to animals kept at 34 and 40‰. The analysis of haemocyte size frequency distribution highlighted that in clams kept at 28‰ the haemocyte fraction of about 5 μm in diameter and 50–100 femtolitre in volume increased markedly. Conversely, in animals kept at 40‰ an increase was observed in the haemocyte fraction having about 8–10 μm diameter and 400–500 femtolitre volume. Higher phagocytic activity was recorded in haemocytes from control clams, with respect to that of clams kept at 28 and 34‰. Lysozyme-like activity in haemocyte lysate was shown to increase significantly in animals kept at 28‰ with respect to that of clams kept at 40‰, whereas enzyme activity in cell-free haemolymph from clams kept at 34‰ was significantly higher with respect to that of clams maintained at 40‰. A relationship between phagocytosis and lysozyme secretion is suggested. The resistance to air exposure of clams kept at 28 and 40‰ was shown to decrease significantly; LT50 values fell from 7 days in clams kept at 34‰ to 4 and 5 days in those kept at 28 and 40‰, respectively. Results demonstrated that salinity values far from 34‰ affects the functional responses of haemocytes and reduce the resistance of clams to exposure to air.

Keywords

Bivalve Phagocytic Activity Lysozyme Activity Experimental Salinity Total Haemocyte Count 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants from the Italian MiPAF to Dr. O. Cattani (contr. n° 6C66). The authors wish to thank Keith Smith for revising the English text.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Valerio Matozzo
    • 1
  • Marta Monari
    • 2
  • Jurgen Foschi
    • 2
  • Gian Paolo Serrazanetti
    • 2
  • Otello Cattani
    • 2
  • Maria Gabriella Marin
    • 1
  1. 1.Department of BiologyUniversity of PadovaPadovaItaly
  2. 2.Department of Biochemistry “G. Moruzzi”University of BolognaOzzano Emilia, BolognaItaly

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