The Effect of Dried Beancurd on Bone Mineral Density in Postmenopausal Chinese Women: A 2-Year Randomized Controlled Trial

  • Lei LiEmail author
  • Meijun Sun
  • Jianping Sun
  • Hangru Kong
  • Wenwen ZhongEmail author
  • Hong Wang
Original Research


Soy foods contain several components such as isoflavones, calcium and protein that potentially modulate bone turnover and increase bone mineral density (BMD) in postmenopausal women. The study is to evaluate the effect of dried beancurd supplementation on skeletal health in postmenopausal Chinese women. Three hundred postmenopausal women aged 50–65 years were assigned into two groups, receiving 100 g dried beancurd or rice cake a day for 2 years. BMD at the lumbar spine and right proximal femur were measured with a dual-energy X-ray absorptiometry. The bone turnover biomarkers of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and urinary N-telopeptide cross-links of collagen normalized for creatinine (NTX/CRT) were also determined. Serum isoflavone concentration was analyzed by high performance liquid chromatography. The 2-year dried beancurd supplementation generated a significant increase in lumbar spine BMD. An obvious decrease was found in urinary NTX/CRT, and a significant increase was detected in serum isoflavone concentration. The dried beancurd supplementation had no effect on changes of right proximal femur BMD and concentrations of serum ALP and BGP. Daily supplementation of dried beancurd could increase BMD of lumbar spine, but does not slow bone loss at right proximal femur in postmenopausal Chinese women.


Dried beancurd Postmenopausal women BMD Calcium Isoflavones 



We appreciate the support of all the participants involved in the study. We are grateful to the Nestlé Foundation for the funding that made the entire work possible.

Author Contributions

Author LL designed the study and prepared the first draft of the paper. She is guarantor. Authors MS, JS, HK and WZ contributed to the human study and experimental work. Author HW was responsible for statistical analysis of the data. All authors revised the paper critically for intellectual content and approved the final version. All authors agreed to be accountable for the work and to ensure that any questions relating to the accuracy and integrity of the paper were investigated and properly resolved.


This study was funded by Nestlé Foundation.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Human and Animal Rights and Informed Consent

The study was conducted in accordance with the ethical standards of Institutional Review Committee of Public Health School of Xiamen University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all participants included in the study.

Supplementary material

223_2019_604_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Public Health SchoolXiamen UniversityXiamenChina
  2. 2.Qingdao Center for Disease Control and PreventionQingdaoChina
  3. 3.General Hospital of People’s Liberation ArmyBeijingChina

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