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Calcified Tissue International

, Volume 104, Issue 3, pp 301–312 | Cite as

Effect of Adding a Galacto-Oligosaccharides/Fructo-Oligosaccharides (GOS/FOS®) Mixture to a Normal and Low Calcium Diet, on Calcium Absorption and Bone Health in Ovariectomy-Induced Osteopenic Rats

  • Mariana Seijo
  • Gabriel Bryk
  • Magalí Zeni Coronel
  • Marina Bonanno
  • María Esther Río
  • María Luz Pita Martín de Portela
  • Susana Noemí ZeniEmail author
Original Research
  • 94 Downloads

Abstract

Menopause is associated with bone loss. Prebiotics increase Ca, inorganic phosphorus (Pi), and Mg absorption, improving bone health. These increases would supply an extra amount of minerals, decreasing bone resorption and possibly reversing ovariectomy-induced bone loss. The present experimental study sought to evaluate the effect of adding a prebiotic GOS/FOS® mixture to a normal or a low Ca diet on Ca, Pi, and Mg absorption, in osteopenic rats. Four groups of n = 8 rats each were OVX, and 8 rats were SHAM operated. All rats were fed a commercial diet for 45 days. They were then fed one of the following diet for 45 days: C-0.5%: SHAM fed AIN 93 M containing 0.5%Ca; O-0.5% and O-0.3%: OVX rats fed AIN 93 M, containing 0.5% or 0.3%Ca, respectively; GF-0.5% and GF-0.3%: OVX rats fed AIN 93 M, containing 0.5% or 0.3%Ca+ 2.5% GOS/FOS®, respectively. At the end of the experimental time point, Ca, P, and MgAbs% was significantly higher in GF-0.5% and GF-0.3% as compared to the remaining groups (p < 0.01). Irrespective of diet Ca content, CTX decreased whereas femur Ca and P content, tibia BV/TV and GPC.Th, lumbar spine and proximal tibia BMD, bone strength, bone stiffness, and elastic modulus increased in the GF-0.5% and GF-0.3% groups as compared to O-0.5% and O-0.3%, respectively (p < 0.05). This prebiotic mixture would be a useful tool to prevent the increase in bone loss associated with menopause and aging.

Keywords

Rats Bone loss Prebiotics Calcium absorption and retention 

Abbreviations

OVX

Ovariectomized

SHAM

Simulated operation

EGP

Epiphyseal growth plate

Pi

Inorganic phosphorus

CaI

Calcium intake

NDFO

Non-digestible fructo-oligosaccharides

GOS

Galacto-oligosaccharides

FOS

Long-chain fructo-oligosaccharides

AIN

American Institute of Nutrition

NCa

Normal Ca content diet

LCa

Low Ca content diet

GF groups

GOS/FOS groups

O groups

Ovariectomized groups

BW

Body weight

CO2

Oxygen dioxide

LS

Lactobacillus

CFU

Colony forming units

I

Food intake

F

Feces

Abs

Apparent mineral absorption

Mg

Magnesium

HCl

Hydrochloric acid

HNO3

Nitric acid

CTX

C-terminal telopeptide of collagen type I

BAP

Bone alkaline phosphatase

tsBMC

Total skeleton bone mineral content

tsBMD

Total skeleton bone mineral density

DXA

Dual energy X-ray absorptiometry

CV

Coefficients of variation

ROI

Region of interest

EDTA

Ethylene-diamine-tetra-acetic acid

BV/TV

Bone volume fraction

GPC.Th

Total width of epiphyseal cartilage

Notes

Acknowledgements

The authors thank technicians Ricardo Orzuza and Julia Somoza for their technical assistance.

Author Contributions

This study is part of the thesis of MS who participated in all stages of the experiment, data entry, and analysis of results. GB performed the biochemical determinations, and MZC participated in the anthropometric and food evaluations. MER and MLPMP participated in the design of the study and in animal and diet control, and SNZ was the director of the investigation.

Funding

This study was supported by the University of Buenos Aires and CONICET. This study was funded by UBACyT 20020090200037 and PIP funding programs.

Compliance with Ethical Standards

Conflict of interest

Mariana Seijo, Gabriel Bryk, Magalí Zeni Coronel, Marina Bonanno, María Esther Rio, María Luz Pita Martín de Portela and Susana Noemí Zeni have no conflict of interest to declare.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Mariana Seijo
    • 1
  • Gabriel Bryk
    • 1
    • 2
  • Magalí Zeni Coronel
    • 1
  • Marina Bonanno
    • 1
  • María Esther Río
    • 3
  • María Luz Pita Martín de Portela
    • 4
  • Susana Noemí Zeni
    • 1
    • 2
    Email author
  1. 1.Metabolic Bone Diseases Laboratory, Clinical Hospital, Immunology, Genetic and Metabolism Institute (INIGEM)National Council for Scientific and Technologic Research (CONICET), Buenos Aires University (UBA)Buenos AiresArgentina
  2. 2.General and Oral Biochemistry Department, School of DentistryBuenos Aires University (UBA)Buenos AiresArgentina
  3. 3.National Council for Scientific and Technologic Research (CONICET)Buenos AiresArgentina
  4. 4.Food Science and Nutrition Department, School of Pharmacy and BiochemistryBuenos Aires University (UBA)Buenos AiresArgentina

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