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Recurrent antinociception induced by intrathecal or peripheral oxytocin in a neuropathic pain rat model

  • Abimael González-Hernández
  • Antonio Espinosa De Los Monteros-Zuñiga
  • Guadalupe Martínez-Lorenzana
  • Miguel Condés-LaraEmail author
Research Article
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Abstract

The search for new ligands to treat neuropathic pain remains a challenge. Recently, oxytocin has emerged as an interesting molecule modulating nociception at central and peripheral levels, but no attempt has been made to evaluate the effect of recurrent oxytocin administration in neuropathic pain. Using male Wistar rats with spinal nerve ligation, we evaluated the effects of recurrent spinal (1 nmol; given by lumbar puncture) or peripheral (31 nmol; given by intraplantar injection in the ipsilateral paw to spinal nerve ligation) oxytocin administration on pain-like behavior in several nociceptive tests (tactile allodynia and thermal and mechanical hyperalgesia) on different days. Furthermore, we used an electrophysiological approach to analyze the effect of spinal 1 nmol oxytocin on the activity of spinal dorsal horn wide dynamic range cells. In neuropathic rats, spinal or peripheral oxytocin partially restored the nociceptive threshold measured with the von Frey filaments (tactile allodynia), Hargreaves (thermal hyperalgesia) and Randall–Selitto (mechanical hyperalgesia) tests for 12 days. These results agree with electrophysiological data showing that spinal oxytocin diminishes the neuronal firing of the WDR neurons evoked by peripheral stimulation. This effect was associated with a decline in the activity of primary afferent Aδ- and C-fibers. The above findings show that repeated spinal or peripheral oxytocin administration attenuates the pain-like behavior in a well-established model of neuropathic pain. This study provides a basis for addressing the therapeutic relevance of oxytocin in chronic pain conditions.

Keywords

Pain Neuropathic Oxytocin 

Notes

Acknowledgements

The authors thank Jessica González Norris for proofreading the manuscript. This study was supported by the Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-UNAM Mexico) under Grant agreement no. IN200415 to MC-L and Grants no. IA203117 and IA203119 to AG-H. AEM-Z is a Doctoral student from Programa de Doctorado en Ciencias Biomédicas (PDCB-UNAM) and received fellowship from Consejo Nacional de Ciencia y Tecnología (CONACyT-Mexico).

Author contributions

AEM-Z contributed to the acquisition, analysis and interpretation of data and participated in drafting the manuscript. AG-H, GM-L and MC-L contributed to the conception and design of the study; acquisition, analysis and interpretation of data; and participated in drafting the manuscript. All authors participated in a critical review of the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. 1.Departamento de Neurobiología del Desarrollo y Neurofisiología, Instituto de NeurobiologíaUniversidad Nacional Autónoma de MéxicoQuerétaroMexico

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