Intracranial landmarks and other techniques to further improve the precision of stereotaxic tracer injections
This study is meant to combine traditional aspects of tracer microinjections using bone landmarks like bregma and lambda with novel procedures in which specific parts of the brain can serve as reference points. For telencephalic and diencephalic injections, the brain surface, the interhemispheric groove and the straight sinus can be used as absolute zero points for dorso-ventral, medio-lateral and rostro-caudal coordinates, respectively. In case of brainstem targets, the surface of the rhomboid fossa, the posterior spinal artery and the obex could serve as reference points along the above-mentioned coordinates. The application of high-precision stereotaxic measurements based on intracranial landmarks and sophisticated surgical procedures can yield well-targeted, small and well circumscribed injection sites that make possible the mapping of discrete nuclear subdivisions or delicate nuclei in the brain.
KeywordsCranial landmarks Intracranial landmarks Straight sinus Obex Virus microinjections Brain mapping Interhemispheric groove/fissure
This research was supported by grants from the Hungarian Science Research Foundation, OTKA TS-049861. Special thanks to, Zsuzsanna E. Tóth PhD, Zoltán Buzogány D. D. and János Cifra M. D. for providing us substances (ZnCl2) and advice.
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- Boldogköi ZS, Sik A, Dénes Á, Reichart A, Toldi J, Gerendai I, Kovács JK, Palkovits M (2004) Novel tracing paradigms-genetically engineered herpesviruses as tools for mapping functional circuits within the CNS: present status and future prospects. Prog Neurobiol 72:417–445PubMedCrossRefGoogle Scholar
- Gerfen CR, Sawchenko PE (1984) An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus Vulgaris Leucoagglutinin (PHA-L). Brain Res 290:219–238PubMedCrossRefGoogle Scholar
- Gildenberg LP (1988) Stereotactic surgery: present and past stereotactic neurosurgery. Williams and Wilkins, BaltimoreGoogle Scholar
- Kelly JP (1991) Introduction and historical aspects tumor stereotaxis. W.B. Saunders Company, PhiladelphiaGoogle Scholar
- Levy R (1992) A short history of stereotactic surgery. Cyber Mus Neurosurg. http://www.neurosurgery.org/cybermuseum/stereotactichall/stereoarticle.html. Accessed 01 March 2010
- Paxinos G, Franklin KBJ (1997) The mouse brain in stereotaxic coordinates. Academic Press, SydneyGoogle Scholar
- Paxinos G, Watson C (2005) The rat brain in stereotaxic coordinates, 5th edn. Academic Press, SydneyGoogle Scholar
- Swanson LW (2004). Brain maps: structure of the rat brain, 3rd edn. Elsevier, AmsterdamGoogle Scholar