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Use of strain Hanseniaspora guilliermondii BF1 for winemaking process of white grapes Vitis vinifera cv Fiano

Abstract

In industrial winery, the use of mixed starter cultures composed of Saccharomyces and non-Saccharomyces yeasts is an approach of growing importance for winemakers to enhance the sensory and complexity of the wine without compromising the quality. In this work, the oenological properties and enzymatic activities of 196 non-Saccharomyces yeasts, belonging to Hanseniaspora guilliermondii and Hanseniaspora uvarum species, were investigated. This screening has allowed the selection of the best non-Saccharomyces yeast strain and the use of vinification for white grape of Campania Vitis vinifera cv Fiano, in an industrial scale. The experimental fermentations were performed in four different batches: batch A (H. guilliermondii BF1 and S. cerevisiae 404 in sequential inoculum); batch B only inoculum of S. cerevisiae 404; batch C without inoculation (spontaneous fermentation); batch D with the inoculum of H. guilliermondii BF1 strain. The results of chemical and sensorial analyses have showed that the best wine comes from batch A. In conclusion, the use of H. guilliermondii BF1 with good oenological properties and a strong β-glucosidase activity allowed to improve the sensorial complexity of the wine. Selected non-Saccharomyces yeast could be applied profitably to winemaking to enhance the quality of the wine using new fermentation technologies, and could be used in industrial winery.

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References

  1. 1.

    Heard GM, Flee GH (1986) Evaluation of selective media for enumeration of yeasts during wine fermentation. J Appl Bacteriol 60:77–481

  2. 2.

    Fleet GH (2003) Yeast interactions and wine flavor. Int J Food Microbiol 8:11–22

  3. 3.

    Fleet GH, Lafon-Lafourcade S, Ribereau-Gayon P (1984) Evolution of yeasts and lactic acid bacteria during fermentation and storage of bordeaux wines. Appl Environ Microbiol 48:1034–1038

  4. 4.

    Kunkee RE (1984) Selection and modification of yeasts and lactic acid bacteria for wine fermentation. Food Microbiol 1:315–332

  5. 5.

    Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma—a review. Institute for Wine Biotechnology and Department of Viticulture & Oenology, University of Stellenbosch, Private Bag XI, 7602 Matieland (Stellenbosch), South Africa

  6. 6.

    Romano P, Fiore C, Paraggio M, Caruso M, Capece A (2003) Function of yeast species and strains in wine flavor. Int J Food Microbiol 86:169–180

  7. 7.

    Fleet GH (1990) Growth of yeasts during wine fermentations. J Wine Res 1:211–223

  8. 8.

    Ciani M, Beco L, Comitini F (2006) Fermentation behaviour and metabolic interactions of multistarter wine yeast fermentations. Int J Food Microbiol 108:239–245

  9. 9.

    Goto S (1980) Changes in the wild yeast flora of sulphited grape musts. J Inst Enol Vitic Yamana-shi Univ 15:29–32

  10. 10.

    Martìnez E, Perez Rodrìguez L, Benìtez T (1997) Evolution of flor yeast population during the biological aging of fino Sherry wine. Am J Enol Vitic 45:1967–1971

  11. 11.

    Fleet GH (2003) Yeast interactions and wine flavor. Int J Food Microbiol 86:11–22

  12. 12.

    Pina C, Santos C, Couto AJ, Hogg T (2004) Ethanol tolerance of five non-Saccharomyces wine yeasts in comparison with a strain of Saccharomyces cerevisiae influence of different culture conditions. Food Microbiol 21:439–447

  13. 13.

    Jolly NP, Varela C, Pretorius IS (2014) Not your ordinary yeast: non-Saccharomyces yeasts in wine production uncovered. FEMS Yeast Res 14:215–237

  14. 14.

    Moreira N, Mendes F, Hogg T, Vasconcelos I (2005) Alcohols, esters and heavy sulphur compounds production by pure and mixed cultures of apiculate wine yeasts. Int J Food Microbiol 103:285–294

  15. 15.

    Martin V, Valera M, Medina K, Boido E, Carrau F (2018) Oenological impact of the Hanseniaspora/Kloeckera yeast genus on wines—a review. Fermentation 4:76

  16. 16.

    Ganga MA, Martınez C (2004) Effect of wine yeast monoculture practice on the biodiversity of non-Saccharomyces yeasts. J Appl Microbiol 96:76–83

  17. 17.

    Hu K, Jin GJ, Xu YH, Tao YS (2018) Wine aroma response to different participation of selected Hanseniaspora uvarum in mixed fermentation with Saccharomyces cerevisiae. Food Res Int 108:119–127

  18. 18.

    Garijo P, González-Arenzana L, López-Alfaro I, Garde-Cerdán T, López R, Santamaría P, Gutiérrez AR (2014) Analysis of grapes and the first stages of the vinification process in wine contamination with Brettanomyces bruxellensis. Eur Food Res Technol 240:525–532

  19. 19.

    López S, Mateo J, Maicas S (2016) Screening of Hanseniaspora strains for the production of enzymes with potential interest for winemaking. Fermentation 2:1

  20. 20.

    Succi M, Pannella G, Tremonte P, Tipaldi L, Coppola R, Iorizzo M, Lombardi SJ, Sorrentino E (2017) Sub-optimal pH preadaptation improves the survival of Lactobacillus plantarum strains and the malic acid consumption in wine-like medium. Front Microbiol 8:470

  21. 21.

    Gunata YZ, Dugelay I, Sapis JC, Baumes R, Bayonove C (1994) Role of enzymes in the use of the flavor potential from grape glycosides in winemaking. In: Schreier P, Winter-halter P (eds) Progress in flavor precursor studies. Proceedings of the international conference. Wurzburg, Germany

  22. 22.

    De Leonardis A, Testa B, Macciola E, Lombardi SJ, Iorizzo M (2016) Exploring enzyme and microbial technology for the preparation of green table olives. Eur Food Res Technol 242:363–370

  23. 23.

    Laffort JF, Romat H, Darriet P (1989) Les levures et l’expression aromatique des vins blancs. Rev Oenol 53:9–12

  24. 24.

    Villena MA, Iranzo JFU, Pèrez AIB (2007) β-Glucosidase activity in wine yeasts: application in enology. Enzyme Microb Technol 40:420–425

  25. 25.

    Strauss MLA, Jolly NP, Lambrechts MG, Van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol 91:182–190

  26. 26.

    Parker M, Capone DL, Francis IL, Herderich MJ (2018) Aroma precursors in grapes and wine: flavor release during wine production and consumption. J Agric Food Chem 66:2281–2286

  27. 27.

    Iorizzo M, Testa B, Lombardi SJ, García-Ruiz A, Munoz-Gonzalez C, Bartolome B, Moreno-Arribas MV (2016) Selection and technological potential of Lactobacillus plantarum bacteria suitable for wine malolactic fermentation and grape aroma release. Food Sci Technol 73:557–566

  28. 28.

    Ma D, Yanb X, Wanga Q, Zhang Y, Tao Y (2017) Performance of selected P. fermentans and its exocellular enzyme in co-inoculation with S. cerevisiae for wine aroma enhancement. LWT Food Sci Technol 86:361–370

  29. 29.

    Medina K, Boido E, Fariña L, Gioia O, Gomez ME, Barquet M, Gaggero C, Dellacassa E, Carrau F (2013) Increased flavour diversity of Chardonnay wines by spontaneous fermentation and co-fermentation with Hanseniaspora vineae. Food Chem 141:2513–2521

  30. 30.

    Čuš F, Zabukovec P, Schroers HJ (2017) Indigenous yeasts perform alcoholic fermentation and produce aroma compounds in wine. Czech J Food Sci 35:339–345

  31. 31.

    Ugliano M, Moio L (2008) Free and hydrolytically released volatile compounds of Vitis vinifera L. cv. Fiano grapes as odour-active constituents of Fiano wine. Anal Chim Acta 621:79–85

  32. 32.

    Chen K, Escott C, Loira I, Del Fresno JM, Morata A, Tesfaye W, Calderon F, Suarez-Lepe JA, Han S, Benito S (2018) Use of non-Saccharomyces yeasts and oenological tannin in red winemaking: influence on colour, aroma and sensorial properties of young wines. Food Microbiol 69:51–63

  33. 33.

    Community E (1990) Methods for the analysis of wines. Off J Eur Commun Regul 2676(90):1–192

  34. 34.

    Oliveira RQ, Rosa CA, Uetanabaro APT, Azeredo A, Neto AG, Assis SA (2009) Polygalacturonase secreted by yeasts from Brazilian semi-arid environments. Int J Food Sci Nutr 60:72–80

  35. 35.

    Parkkinen E, Oura E, Suomalainen H (1978) Esterases of baker’s yeast. I. Activity and localization in the yeast cell. J Inst Brew 84:5–8

  36. 36.

    Bilinski CA, Russel I, Stewart G (1987) Applicability of yeast extracellular proteinases in brewing: physiological and biochemical aspects. Appl Environ Microbiol 53:495–499

  37. 37.

    Teather RM, Wood PJ (1982) Use of Congo red–polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780

  38. 38.

    Rosi I, Vinella M, Domizio P (1994) Characterization of β-glucosidase activity in yeasts of oenological origin. J Appl Bacteriol 77:519–527

  39. 39.

    Hernandez LF, Espinosa JC, Fernandez-Gonzalez M, Briones A (2003) β-Glucosidase activity in a Saccharomyces cerevisiae wine strain. Int J Food Microbiol 80:171–176

  40. 40.

    Chassagne D, Vernizeau S, Nedjma M, Hervé A (2005) Hydrolysis and sorption by Saccharomyces cerevisiae strains of Chardonnay grape must glycosides during fermentation. Enzyme Microb Technol 37:212–217

  41. 41.

    Cavazza A, Grando MS, Zini C (1992) Rivelazione della microflora microbica di mosti e vini. Vignevini 9:17–20

  42. 42.

    Pallmann CL, Brown JA, Olineka TL, Cocolin L, Mills DA, Bisson LF (2001) Use of WL medium to profile native flora fermentations. Am J Enol Vitic 52:198–203

  43. 43.

    Barnett JA, Payne RW, Yarrow D (1990) Yeasts: characteristics and identification. Cambridge University Press, Cambridge

  44. 44.

    Cocolin L, Manzano M, Cantoni C, Comi G (2001) Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages. Appl Environ Microbiol 67:5113–5121

  45. 45.

    Testa B, Lombardi SJ, Tremonte P, Succi M, Tipaldi L, Pannella G, Sorrentino E, Iorizzo M, Coppola R (2014) Biodiversity of Lactobacillus plantarum from traditional Italian wines. World J Microbiol Biotechnol 30:2299–2305

  46. 46.

    Singleton VL, Orthofer R, Lamuela-Raventós RM. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 299:152–178

  47. 47.

    International Organisation of Vine and Wine, Compendium of international methods of wine and must analysis, 1, ISBN Volume I: 979-10-91799-19-5, OIV-MA-INT-00-2014, Edition 2014

  48. 48.

    Iorizzo M, Macciola V, Testa B, Lombardi SJ, De Leonardis A (2014) Physicochemical and sensory characteristics of red wines from the rediscovered autochthonous Tintilia grapevine grown in the Molise region (Italy). Eur Food Res Technol 238:1037–1048

  49. 49.

    De Leonardis A, Macciola V, Iorizzo M, Lombardi SJ, Lopez F, Marconi E (2018) Effective assay for olive vinegar production from olive oil mill wastewaters. Food Chem 240:437–440

  50. 50.

    Fleet GH (1993) Wine microbiology and biotechnology. Harwood Academic Publishers, Chur

  51. 51.

    Charoenchai C, Fleet GH, Henschke PA, Todd BEN (1997) Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust J Grape Wine Res 3:2–8

  52. 52.

    Lombardi SJ, De Leonardis A, Lustrato G, Testa B, Iorizzo M (2015) Yeast autolysis in sparkling wine aging: use of killer and sensitive Saccharomyces cerevisiae strains in co-culture. Recent Pat Biotechnol 9:000–000

  53. 53.

    Peinado RA, Moreno JA, Muñoz D, Medina M, Moreno J (2004) Gas chromatographic quantification of major volatile compounds and polyols in wine by direct injection. J Agric Food Chem 52:6389–6393

  54. 54.

    Ferreira V, Lopez R, Cacho JF (2000) Quantitative determination of the odorants of young red wines from different grape varieties. J Sci Food Agric 80:1659–1667

  55. 55.

    Zhang S, Petersen MA, Liu J, Toldam-Andersen TB (2015) Influence of pre-fermentation treatments on wine volatile and sensory profile of the new disease tolerant cultivar solaris. Molecules 20:21609–21625

  56. 56.

    Tristezza M, Tufariello M, Capozzi V, Spano G, Mita G, Grieco F (2016) The oenological potential of Hanseniaspora uvarum in simultaneous and sequential co-fermentation with Saccharomyces cerevisiae for industrial wine production. Front Microbiol 7:670

  57. 57.

    Lombardi SJ, Pannella G, Iorizzo M, Moreno-Arribas MV, Tremonte P, Succi M, Sorrentino E, Macciola V, Di Renzo M, Coppola R (2018) Sequential inoculum of Hanseniaspora guilliermondii and Saccharomyces cerevisiae for winemaking Campanino on an industrial scale. World J Microbiol Biotechnol 34:161

  58. 58.

    Viana F, Gil JV, Genovés S, Vallés S, Manzanares P (2008) Rational selection of non-Saccharomyces wine yeasts for mixed starters based on ester formation and enological traits. Food Microbiol 25:778–785

  59. 59.

    Ciani M, Comitini F, Mannazzu I, Domizio P (2010) Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. FEMS Yeast Res 10:123–133

  60. 60.

    Capozzi V, Garofalo C, Chiriatti MA, Grieco F, Spano G (2015) Microbial terroir and food innovation: the case of yeast biodiversity in wine. Microbiol Res 181:75–83. https://doi.org/10.1016/j.micres.2015.10.005

  61. 61.

    Masneuf-Pomarede I, Bely M, Marullo P, Albertin W (2016) The genetics of non-conventional wine yeasts: current knowledge and future challenges. Front Microbiol 6:1563. https://doi.org/10.3389/fmicb.2015.01563

  62. 62.

    Carrau F, Gaggero C, Aguilar PS (2015) Yeast diversity and native vigor for flavor phenotypes. Trends Biotechnol 33(3):148–154

  63. 63.

    Rantsiou K, Dolci P, Giacosa S, Torchio F, Tofalo R, Torriani S (2012) Candida zemplinina can reduce acetic acid produced by Saccharomyces cerevisiae in sweet wine fermentations. Appl Environ Microbiol 78:1987–1994. https://doi.org/10.1128/AEM.06768-11

  64. 64.

    Jolly NP, Augustyn OPH, Pretorius IS (2006) The role and use of non-Saccharomyces yeasts in wine production. S Afr J Enol Vitic 27:15–38

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Correspondence to Massimo Iorizzo.

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Testa, B., Lombardi, S.J., Iorizzo, M. et al. Use of strain Hanseniaspora guilliermondii BF1 for winemaking process of white grapes Vitis vinifera cv Fiano. Eur Food Res Technol 246, 549–561 (2020). https://doi.org/10.1007/s00217-019-03424-8

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Keywords

  • Fiano wine
  • Mixed yeasts cultures
  • Hanseniaspora guilliermondii
  • Industrial winery
  • Winemaking