European Food Research and Technology

, Volume 245, Issue 12, pp 2679–2686 | Cite as

Valorization of the agro-forestry wastes from Italian chestnut cultivars for the recovery of bioactive compounds

  • Filomena Monica Vella
  • Luigi De Masi
  • Roberto Calandrelli
  • Alessandra Morana
  • Bruna LarattaEmail author
Original Paper


Chestnut is a very promising forest species that may be used as biomass to recover high value-added biomolecules. Four cultivars coming from the Italian Park “Roccamonfina-Foce Garigliano” were characterized at biochemical level by phenolic chromatographic profiling and by means of antioxidant activity. The phenolic compounds were extracted from leaves, burs, and shells for all cultivars and the inner shells resulted the richest by-product in all varieties. Comparison of data sets using principal component analysis led to conclusions regarding the biochemical relationships among all cultivars. Furthermore, cluster analysis with HPLC data on the phytochemical compounds produced a dendrogram showing the relationships within Italian cultivars, so confirming the same pattern showed in PCA. In conclusion, high added-value bioactive compounds, utilized as dietary and pharmaceutical supplements, can be achieved from non-edible chestnut components discarded during post-harvest and food processing, so improving the valuable capacity of recycling of chestnut wastes.


Castanea sativa By-products Antioxidant activity Bioactive compounds Waste valorization 



The authors gratefully thank the “Verde Collina” association of the Regional Park of “Roccamonfina-Foce Garigliano” with Mr. Mario Conti who provided chestnut samples for this study, and Dr. Speranza Panico to have carried out some experiments related to her thesis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.

Supplementary material

217_2019_3379_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 14 kb)


  1. 1.
    Faostat, Food and Agriculture Organization of the United States (2017). Accessed 6 May 2017
  2. 2.
    Pantera A, Burgess PJ, Mosquera Losada R, Moreno G, López-Díaz ML, Corroyer N, McAdam J, Rosati A, Papadopoulos AM, Graves A, Rigueiro Rodríguez A, Ferreiro-Domínguez N, Fernández Lorenzo JL, González-Hernández MP, Papanastasis VP, Mantzanas K, Van Lerberghe P, Malignier N (2018) Agroforestry for high value tree systems in Europe. Agroforest Syst 92:945–959CrossRefGoogle Scholar
  3. 3.
    De Vasconcelos MCBM, Bennett RN, Rosa EAS, Ferreira-Cardoso JV (2010) Composition of European chestnut (Castanea sativa Mill.) and association with health effects: fresh and processed products. J Sci Food Agr 90:1578–1589CrossRefGoogle Scholar
  4. 4.
    Maurelli L, Ionata E, La Cara F, Morana A (2013) Chestnut shell as unexploited source of fermentable sugars: effect of different pretreatment methods on enzymatic saccharification. Appl Biochem Biotechnol 170(5):1104–1118CrossRefGoogle Scholar
  5. 5.
    Braga N, Rodrigues F, Oliveira MBPP (2015) Castanea sativa by-products: a review on added value and sustainable application. Nat Prod Res 29:1–18CrossRefGoogle Scholar
  6. 6.
    Laufenberg G, Kunz B, Nystroem M (2003) Transformation of vegetable waste into value added products: (A) the upgrading concept, (B) practical implementations. Bioresour Technol 87:167–198CrossRefGoogle Scholar
  7. 7.
    Gondar H, Romane F, Santa Regina I, Leonardi S (2006) Forest management and plant species diversity in chestnut stands of three Mediterranean areas. Springer, Dordrecht, pp 69–82Google Scholar
  8. 8.
    Kosseva MR (2009) Processing of food wastes. Adv Food Nutr Res 58:57–136CrossRefGoogle Scholar
  9. 9.
    Schieber A, Stintzin FC, Carle R (2001) By-products of plant food processing as a source of functional compounds—recent developments. Trends Food Sci Technol 12:401–413CrossRefGoogle Scholar
  10. 10.
    Galanakis CM (2012) Recovery of high added-value components from food wastes: conventional, emerging technologies and commercialized applications. Trends Food Sci Technol 26:68–87CrossRefGoogle Scholar
  11. 11.
    De Vasconcelos MCBM, Bennett RN, Quideau S, Jacquet R, Rosa EAS, Ferreira-Cardoso JV (2010) Evaluating the potential of chestnut (Castanea sativa Mill.) fruit pericarp and integument as a source of tocopherols, pigments and polyphenols. Ind Crops Prod 31:301–311CrossRefGoogle Scholar
  12. 12.
    Pinto D, Rodrigues F, Braga N, Santos J, Pimentel FB, Palmeira-de-Oliveira A, Oliveira MBP (2017) The Castanea sativa bur as a new potential ingredient for nutraceutical and cosmetic outcomes: preliminary studies. Food Funct 8:201–208CrossRefGoogle Scholar
  13. 13.
    Barreira J, Ferreira I, Oliveira M, Pereira J (2008) Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem 107:1106–1113CrossRefGoogle Scholar
  14. 14.
    Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10):7313–7352CrossRefGoogle Scholar
  15. 15.
    Mujić A, Grdović N, Mujić I, Mihailović M, Živković J, Poznanović G, Vidaković M (2011) Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells. Food Chem 125:841–849CrossRefGoogle Scholar
  16. 16.
    Vella FM, Laratta B, La Cara F, Morana A (2018) Recovery of bioactive molecules from chestnut (Castanea sativa Mill.) by-products through extraction by different solvents. Nat Prod Res 32:1022–1032CrossRefGoogle Scholar
  17. 17.
    Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2:270–278CrossRefGoogle Scholar
  18. 18.
    Dini F, Sartor C, Botta R (2012) Detection of a hypersensitive reaction in the chestnut hybrid ‘Bouche de Bétizac’ infested by Dryocosmus kuriphilus Yasumatsu. Plant Physiol Biochem 60:67–73CrossRefGoogle Scholar
  19. 19.
    Singleton VL, Rossi JA Jr (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–158Google Scholar
  20. 20.
    Arnow LE (1937) Colorimetric determination of the components of 3,4-dihydroxyphenylalaninetyrosine mixtures. J Biol Chem 118:531–537Google Scholar
  21. 21.
    Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559CrossRefGoogle Scholar
  22. 22.
    Fernández-Agulló A, Freire MS, Antorrena G, Pereira JA, Gonzàlez-Alvarez J (2014) Effect of the extraction technique and operational conditions on the recovery of bioactive compounds from chestnut (Castanea sativa) bur and shell. Separ Sci Technol 49(2):267–277CrossRefGoogle Scholar
  23. 23.
    Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature 181:1199–1200CrossRefGoogle Scholar
  24. 24.
    Sneath PHA, Sokal RR (1973) Numerical taxonomy. The principles and practice of numerical classification. W.H. Freeman, San FranciscoGoogle Scholar
  25. 25.
    Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78:517S–520SCrossRefGoogle Scholar
  26. 26.
    Bilotto S, Spagnuolo C, Russo M, Tedesco I, Laratta B, Russo GL (2013) Dietary phytochemicals in chemoprevention of cancer: an update. Immunol Endocr Metab Agents Med Chem 13:2–24CrossRefGoogle Scholar
  27. 27.
    Zhao S, Liu JY, Chen SY, Shi LL, Liu YJ, Ma C (2011) Antioxidant potential of polyphenols and tannins from burs of Castanea mollissima Blume. Molecules 16:8590–8600CrossRefGoogle Scholar
  28. 28.
    Conde E, Moure A, Domínguez H, Parajó JC (2011) Production of antioxidants by non-isothermal autohydrolysis of lignocellulosic wastes. LWT-Food Scio Technol 44:436–442CrossRefGoogle Scholar
  29. 29.
    Diaz Reinoso B, Couto D, Mourea A, Fernandes E, Domíngueza H, Parajóa JC (2012) Optimization of antioxidants—extraction from Castanea sativa leaves. Chem Eng J 203(1):101–109CrossRefGoogle Scholar
  30. 30.
    Tomas-Barberan FA, Clifford MN (2000) Dietary hydroxybenzoic acid derivates—nature, occurrence and dietary burden. J Sci Food Agric 80:1024–1032CrossRefGoogle Scholar
  31. 31.
    Manach C, Williamson G, Morand C, Scalbert A, Remesy C (2005) Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr 81:230S–242SCrossRefGoogle Scholar
  32. 32.
    Vekiari SA, Gordon MH, Garcia-Macias P, Labrinea H (2008) Extraction and determination of ellagic acid content in chestnut bark and fruit. Food Chem 110:1007–1011CrossRefGoogle Scholar
  33. 33.
    Jung BS, Lee NK, Na DS, Yu HH, Paik HD (2016) Comparative analysis of the antioxidant and anticancer activities of chestnut inner shell extracts prepared with various solvents. J Sci Food Agric 96(6):2097–2102CrossRefGoogle Scholar
  34. 34.
    Almeida IF, Costa PC, Bahia MF (2009) Evaluation of functional stability and batch-to-batch reproducibility of a Castanea sativa leaf extract with antioxidant activity. AAPS PharmSciTech 2:120–125Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Filomena Monica Vella
    • 1
  • Luigi De Masi
    • 2
  • Roberto Calandrelli
    • 1
  • Alessandra Morana
    • 1
  • Bruna Laratta
    • 1
    Email author
  1. 1.Consiglio Nazionale delle Ricerche (CNR)Istituto di Ricerca sugli Ecosistemi Terrestri (IRET)NaplesItaly
  2. 2.Consiglio Nazionale delle Ricerche (CNR), Istituto di Bioscienze e Biorisorse (IBBR)NaplesItaly

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