On the suitability of alternative cereals, pseudocereals and pulses in the production of alcohol-reduced beers by non-conventional yeasts
The growing interest in non-alcoholic and low alcohol beers (NABLAB) has fuelled research into innovative production methods. One means to produce NABLAB is through limited fermentation by non-Saccharomyces yeasts which have a naturally low fermentative capacity in cereal-based wort substrates. At the same time, adjunct brewing, the partial replacement of barley malt on the grain bill, enjoys growing popularity. In this study, 13 cereals, pseudocereals, and pulses were investigated for their suitability to produce a wort with limited amounts of fermentable sugars. Subsequently, the fermentation performance of two non-Saccharomyces yeast strains, namely Cyberlindnera subsufficiens C6.1 and Lachancea fermentati KBI 12.1, in the produced worts was investigated and compared to that of a brewers’ yeast strain. The worts were produced by harnessing endogenous amylolytic enzyme activity or the addition of an external amylase and analysed for their sugar composition and free amino acids (FAA) profile. All alternative substrates without endogenous β-amylase activity were found to be suitable for producing worts with a high proportion of unfermentable sugars. However, the extract yield was low for the pulses and most worts exhibited a low and/or unbalanced FAA profile. The ethanol production was limited and mostly dependent on the sugar spectrum of the worts and the sugar utilization characteristics of the applied yeast strains. The (partial) substitution of barley with alternative substrates when producing NABLAB by non-Saccharomyces yeast can be a means to alter the sugar and FAA profile of the wort, but must be considered in concert with the yeast strains’ characteristics.
KeywordsCereals Pseudocereals Pulses Non-conventional yeast Non-Saccharomyces yeast Low alcohol beer
The authors would like to thank Ms Anne-Sophie Brasseur for her contribution to this study, and Dr Josh Taylor and the Kerry Group for the generous donation of the enzymes used in this study. The authors would also like to thank Dr David De Schutter and Dr Luk Daenen for their review.
This work was supported by the Baillet Latour Fund within the framework of a scholarship for doctoral students.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests.
Compliance with ethics requirements
This article does not contain any studies with animal or human subjects.
- 4.Annemüller G, Manger H-J, Burbidge M (2013) Processing of various adjuncts in beer production: raw grain adjuncts—sugars and sugar syrups—malt substitutes, 1st Englis. VLB, BerlinGoogle Scholar
- 5.Lalor E, Goode D (2010) Brewing with enzymes. In: Whitehurst RJ, van Oort M (eds) Enzymes in food technology, 2nd edn. Wiley, Chichester, pp 163–193Google Scholar
- 8.Patterson CA, Curran J, Der T (2017) Effect of processing on antinutrient compounds in pulses. Cereal Chem 94:2–10. https://doi.org/10.1016/B978-0-323-24273-8.00013-7 CrossRefGoogle Scholar
- 12.Bellut K, Michel M, Hutzler M et al (2019) Investigation into the application of Lachancea fermentati strain KBI 12.1 in low alcohol beer brewing. J Am Soc Brew Chem 77:157–169Google Scholar
- 24.Sarita Singh E (2016) Potential of millets: nutrients composition and health benefits. J Sci Innov Res JSIR 5:46–50Google Scholar
- 26.Goode DL, Arendt EK (2006) Developments in the supply of adjunct materials for brewing. Woodhead Publishing Limited, SawstonGoogle Scholar
- 28.Wijngaard HH, Ulmer HM, Neumann M, Arendt EK (2005) The effect of steeping time on the final malt quality of buckwheat. J Inst Brew 111:275–281. https://doi.org/10.1002/j.2050-0416.2005.tb00683.x CrossRefGoogle Scholar
- 32.Kunze W (2010) Technology brewing & malting, 4th Intern. VLB, BerlinGoogle Scholar
- 35.Phiarais BPN, Mauch A, Schehl BD et al (2010) Processing of a top fermented beer brewed from 100% buckwheat malt with sensory and analytical characterisation. J Inst Brew 116:265–274. https://doi.org/10.1002/j.2050-0416.2010.tb00430.x CrossRefGoogle Scholar
- 42.Kurtzman CP, Fell JW, Boekhout T (2011) The yeasts, a taxonomic study, 5th edn. Elsevier, AmsterdamGoogle Scholar
- 47.Schmelzle A, Lindemann B, Methner F-J (2013) Sensory descriptive analysis and consumer acceptance of non-alcoholic beer. BrewingScience 66:144–153Google Scholar