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Evaluation of the availability of delphinidin and cyanidin-3-O-sambubioside from Hibiscus sabdariffa and 6-gingerol from Zingiber officinale in colon using liquid chromatography and mass spectrometry detection

  • Yassine Oulad El Majdoub
  • Mohammed Diouri
  • Paola Arena
  • Adriana Arigò
  • Francesco CacciolaEmail author
  • Francesca Rigano
  • Paola Dugo
  • Luigi Mondello
Original Paper
  • 11 Downloads

Abstract

The in vivo biological activity of Hibiscus sabdariffa (H.s.) and Zingiber officinale (Z.o.) polyphenols is tightly related to their availability in the site of action. The availability of non-absorbed and intact polyphenols, or partially metabolized, in the colon, would perform, according to in vitro outcomes, a highly antioxidant activity and trapping effect towards toxic molecules (e.g., methylglyoxal), which are locally produced in the colon by the gut microbiota. The aim of this study was to evaluate the in vivo availability of three selected polyphenolic compounds of dried H.s calyces, and fresh Z.o. rhizomes in the Wistar rat colon by liquid chromatography coupled to photodiode array and mass spectrometry detection (HPLC–PDA/ESI–MS). The colon-available polyphenols were extracted through the initial solid–liquid faeces extraction by the employment of a solvent mixture (methanol:water, 60:40 v/v), followed by a solid-phase extraction (SPE) on a C18 cartridge. The main H.s. anthocyanins (cyanidin-3-O-sambubioside, C3S and delphinidin-3-O-sambubioside, D3S) and 6-gingerol of Z.o. were available in the intact form in the colon 12 h after the administration of concentrated aqueous extracts (6% and 4% w/v). 72.15% of the ingested C3S and 76.19% of D3S were available in the colon, in comparison to the low availability of 6-gingerol equal to 1.50%. The duration of these bioactive compounds availability in the colon was limited to 12 h. The anthocyanin and gingerol availability in the colon may favor their absorption into the enterocytes, contributing to the antioxidant potential and health effects.

Keywords

Anthocyanins Gingerol Zingiber officinale Hibiscus sabdariffa Availability Colon 

Notes

Acknowledgements

The authors are thankful to Shimadzu and Merck Life Science Corporations for the continuous support.

Compliance with ethical standards

Conflict of interest

The authors of this work have declared no conflict of interest.

Ethical standards

All animal procedures were in accordance with the Guidelines of Italian Health Minister (D.M: 116192), as well as with the EEC regulations (O.J. of E.C.L. 358/1 12/18/1986).

Supplementary material

217_2019_3358_MOESM1_ESM.docx (727 kb)
Supplementary material 1 (DOCX 726 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yassine Oulad El Majdoub
    • 1
  • Mohammed Diouri
    • 1
  • Paola Arena
    • 2
  • Adriana Arigò
    • 2
  • Francesco Cacciola
    • 3
    Email author
  • Francesca Rigano
    • 4
  • Paola Dugo
    • 2
    • 4
    • 5
  • Luigi Mondello
    • 2
    • 4
    • 5
    • 6
  1. 1.Laboratory of Plant Biotechnologies and Molecular Biology, Department of Biology, Faculty of Sciences of MeknesMoulay Ismail UniversityMeknesMorocco
  2. 2.Department of Chemical, Biological, Pharmaceutical and Environmental SciencesUniversity of MessinaMessinaItaly
  3. 3.Department of Biomedical, Dental, Morphological and Functional Imaging SciencesUniversity of MessinaMessinaItaly
  4. 4.Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental SciencesUniversity of MessinaMessinaItaly
  5. 5.Unit of Food Science and Nutrition, Department of MedicineUniversity Campus Bio-Medico of RomeRomeItaly
  6. 6.BeSep s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental SciencesUniversity of MessinaMessinaItaly

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