The CUPRAC-paper microzone plates as a simple and rapid method for total antioxidant capacity determination of plant extract

  • Mochammad Amrun Hidayat
  • Rizka Illa Chassana
  • Indah Yulia Ningsih
  • Mochammad Yuwono
  • Bambang KuswandiEmail author
Short Communication


The current work was aimed to develop the paper microzone plates (PµZP) as a scanometric system for determining the total antioxidant capacity of plant extracts. The PµZP was constructed by immobilizing CUPRAC reagent onto 70-well of patterned paper as a sensing zone for the scanometric detection system. The light blue-sensing zone showed a sensitive response to standard antioxidant (rutin) by forming light yellow color adducts which can be scanned and quantitatively measured by image processing program as a scanometric method. The sensor gave a notable signal at 8 min after rutin addition and presented a linear response in the concentration span of 1–10 mM. The developed method was shown to be reproducible (RSD < 3%) and accurate (98–101% recovery) for determining the TAC of plant extracts (as mM rutin equivalents) and showed strong correlation (r = 0.9887) with the standard CUPRAC method, suggesting that it can be applied for the simple and rapid method for TAC determination.


Antioxidant CUPRAC Paper-based sensor Scanometric Plant extracts 



This work was partially supported by The Ministry of Research, Technology and Higher Education of Indonesia (Grant number 0500/UN25.3.1/LT/2017).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics requirements

The article does not contain any studies with human or animal subjects.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Pharmaceutical Chemistry Department, Faculty of PharmacyAirlangga UniversitySurabayaIndonesia
  2. 2.Chemo and Biosensor Group, Faculty of PharmacyUniversity of JemberJemberIndonesia

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