European Food Research and Technology

, Volume 245, Issue 9, pp 1889–1898 | Cite as

Physico-chemical and nutritional properties of meat analogues based on Spirulina/lupin protein mixtures

  • Megala Palanisamy
  • Stefan Töpfl
  • Ralf G. Berger
  • Christian HertelEmail author
Original Paper


The effect of the addition of Spirulina platensis flour and of extrusion parameters on texture, cooking yield, expressible moisture, total phenolic content (TPC), total flavonoid content (TFC), Trolox equivalent antioxidant activity (TEAC), in vitro protein digestibility (IVPD) and conformational changes of proteins using Fourier-transform infrared spectroscopy (FTIR) of lupin protein based meat analogues was studied. High moisture extrusion (HME) cooking was used to produce the meat analogues. The Spirulina concentration (15, 30 and 50%), extruder barrel temperature (145 °C, 160 °C and 170 °C), water feed (50, 55 and 60%), and screw speed (500, 800 and 1200 rpm) were varied. The Spirulina concentration and extrusion parameters significantly affected physical properties, such as texture, cooking yield and expressible moisture of the extrudates. The addition of Spirulina generally increased the TPC, TFC and TEAC values of the extrudates. Increased temperature and screw speed as well as decreased water feed slightly improved the content of TPC, TFC and TEAC, respectively. The addition of Spirulina at a level of 30% decreased the IVPD of the extrudates from 82 to 75.6%. However, increased water feed and screw speed partly counterbalanced this effect. Protein conformational analyses of the extrudates by FTIR showed that β-sheets were decreased, whereas α-helix, β-turn and antiparallel β-sheets were increased compared to the raw extrusion mixtures. On the whole, the HME process improved the values of TPC, TFC, TEAC and IVPD in the extrudates compared to the raw extrusion mixtures. The addition of Spirulina along with controlled extrusion parameters can deliver meat analogues with improved physico-chemical and nutritional properties.


Spirulina High moisture extrusion Lupin Sustainability 



The authors would like to thank Claus Rüscher, Institut für Mineralogie, Leibniz University Hannover, for helping with the FTIR experiments and data analyses. We also appreciate the assistance of Knut Franke with statistics. This study was supported by the “Niedersächsisches Vorab” programme of the Ministry for Science and Culture of Lower Saxony (Grant # ZN 3041).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Human or animal studies

This article does not contain any studies with human or animal subjects.

Supplementary material

217_2019_3298_MOESM1_ESM.docx (121 kb)
Supplementary material 1 (docx 120 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Megala Palanisamy
    • 1
    • 2
  • Stefan Töpfl
    • 1
  • Ralf G. Berger
    • 2
  • Christian Hertel
    • 1
    Email author
  1. 1.German Institute of Food Technologies (DIL e.V.)QuakenbrueckGermany
  2. 2.Institute of Food ChemistryLeibniz University HannoverHannoverGermany

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