Aging spectral markers of tequila observed by Raman spectroscopy

  • L. I. Espinosa-VegaEmail author
  • A. Belio-Manzano
  • C. A. Mercado-Ornelas
  • I. E. Cortes-Mestizo
  • Victor H. Mendez-Garcia
Original Paper


In this work, were studied the aging spectral markers of three types of tequila: Blanco, Reposado, and Añejo (white, rested, and aged, respectively) by Raman spectroscopy with the aim of qualitatively assessing the aging state of tequilas. The samples were elaborated in the same distillery to increase the correctness of the analysis since the contribution of manufacturing process factors, e.g., the type of water used for the elaboration, the origin of raw materials like agaves, sugar, barrels, and others would be similar for all the kinds of tequila. It was demonstrated that complementary information can be obtained through the employment of two excitation wavelengths: 532 and 785 nm. Fluorescence intensity increases with the aging degree of the tequilas as observed with the 532 nm line providing qualitative information about the resting time within the barrels. Additionally, by employing the 785 nm line it was possible to discern through the νs-CCO νa-CCO bands, (C–O) and (C–H) stretching modes and CH2 wagging bonds, the aging spectral markers of tequila.


Tequila Raman spectroscopy Cask aging Beverage maturation 



The authors acknowledge the financial support from CEMIE-SOL 22, FRC-UASLP and CONACYT-Mexico through Grants: INFR-2015-01-255489, CB 2015-257358 and PNCPN2014-01-248071. Special thanks to “Rancho Palos Colorados” the distillery of “Tequila el Charro” for the information provided to the authors to complete the work presented here.

Compliance with ethical standards

Conflict of interest

Author L. I. Espinosa-Vega declares that she has no conflict of interest. Author A. Belio-Manzano declares that he has no conflict of interest. Author C. A. Mercado-Ornelas declares that he has no conflict of interest. Author I. E. Cortes-Mestizo declares that he has no conflict of interest. Author Victor H. Mendez-Garcia declares that he has no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animals subjects.

Informed consent

Not applicable as this study does not include any human participants.


  1. 1.
    Consejo Regulador del Tequila (2018) Accessed 21 Mar 2018
  2. 2.
    Bautista-Justo M, García-Oropeza L, Barboza-Corona JE, Parra-Negrete LA (2001) El agave tequilana Weber y la producción de tequila. Acta Universitaria 11:26–34Google Scholar
  3. 3.
    Barbosa-García O, Ramos-Ortíz G, Maldonado JL, Pichardo-Molina JL, Meneses-Nava MA, Landgrave JEA, Cervantes-Martínez J (2007) UV–Vis absorption spectroscopy and multivariate analysis as a method to discriminate tequila. Spectrochim Acta Part A 66:129–134CrossRefGoogle Scholar
  4. 4.
    Vo-Dinh T, Allain LR, Stokes DL (2002) Cancer gene detection using surface-enhanced Raman scattering (SERS). J Raman Spectrosc 33:511–516CrossRefGoogle Scholar
  5. 5.
    Van de Poll SWE, Schut TCB, Van der Laarse A, Puppels GJ (2002)) Ceroid in human atherosclerosis by Raman spectroscopy. J Raman Spectrosc 33:544–551CrossRefGoogle Scholar
  6. 6.
    Mahadevan-Jansen A, Richards-Kortum R (1996) Biomed Raman spectroscopy for the detection of cancers and precancers. J Biomed Opt 1(1):31–70CrossRefPubMedGoogle Scholar
  7. 7.
    Caspers PJ, Lucassen GW, Wolthuis R, Bruining HA, Puppels GJ (1998) In vitro and in vivo Raman spectroscopy of human skin. Biospectroscopy 4:S:31–S39CrossRefGoogle Scholar
  8. 8.
    Gnyba M, Smulko J, Kwiatkowski A, Wierzba P (2011) Portable Raman spectrometer—design rules and applications. Bull Pol Acad Sci Tech Sci 59(3):325–329Google Scholar
  9. 9.
    Sanford CL, Mantooth BA, Jones BT (2001) Determination of ethanol in alcohol samples using a modular raman spectrometer. J Chem Educ 78(9):1221CrossRefGoogle Scholar
  10. 10.
    Mendes LS, Oliveira FCC, Suarez PAZ, Rubim JC (2003) Determination of ethanol in fuel ethanol and beverages by Fourier transform (FT)-near infrared and FT-Raman spectrometries. Anal Chim Acta 493(2):219–231CrossRefGoogle Scholar
  11. 11.
    Frausto-Reyes C, Medina-Gutiérrez C, Sato-Berrú R, Sahagún LR (2005) Qualitative study of ethanol content in tequilas by Raman spectroscopy and principal component analysis. Spectrochim Acta Part A 61:2657–2662CrossRefGoogle Scholar
  12. 12.
    Contreras U, Barbosa-García O, Pichardo-Molina JL, Ramos-Ortíz G, Maldonado JL, Meneses-Nava MA, Ornelas-Soto NE, López-de-Alba PL (2010) Screening method for identification of adulterate and fake tequilas by using UV–VIS spectroscopy and chemometrics. Food Res Int 43:2356–2362CrossRefGoogle Scholar
  13. 13.
    Ramírez-Elías MG, Guevara E, Zamora-Pedraza C, Aguirre RJR, Juárez FBI, Bárcenas PGM, Ruiz F, González FJ (2017) Assessment of mezcal aging combining Raman spectroscopy and multivariate analysis techniques. Biomed Spectrosc Imaging 6:75–81CrossRefGoogle Scholar
  14. 14.
    Wolthuis R, Van Aken M, Bruining HA, Puppels GJ (1999) Raman spectroscopic characterization of the liver. SPIE 3608:2–6Google Scholar
  15. 15.
    Schrader B, Dippel B, Erb I, Keller S, Löchte T, Schulz H, Tatsch E, Wessel S (1999) NIR Raman spectroscopy in medicine and biology: results and aspects. J Mol Struct 480:21–32CrossRefGoogle Scholar
  16. 16.
    Ceballos-Magaña SG, Jurado JM, Martín MJ, Pablos F (2009) Quantitation of twelve metals in tequila and mezcal spirits as authenticity parameters. J Agric Food Chem 57:372–1376CrossRefGoogle Scholar
  17. 17.
    Fernandez-Pierna JA, Abbas O, Dardenne P, Baeten V (2011) Discrimination of corsican honey by FT-raman spectroscopy and chemometrics. Biotechnol Agron Soc Environ 15(1):75–84Google Scholar
  18. 18.
    Mosedale JR (1995) Effects of oak wood on the maturation of alcoholic beverages with particular reference to whisky. Forestry (Oxfordjournals.Org) 68:203–230CrossRefGoogle Scholar
  19. 19.
    Mosedale JR, Puech JL (1998) Wood maturation of distilled beverages. Food Sci Technol 9:95–101CrossRefGoogle Scholar
  20. 20.
    Lehtonen M (1983) Gas-liquid chromatographic determination of volatile phenols in matured distilled alcoholic beverages. J Assoc Anal Chem 66:62–70Google Scholar
  21. 21.
    Swan JS, Howie D (1985) Correlation of sensory data in flavour studies into scotch malt whiskies’. In: Nykanen L, Lehtonen P (eds) Proceedings of the alko symposium of flavour research of alcoholic beverages, pp 291–300Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for the Innovation and Application of Science and TechnologyUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico

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