Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plant extracts
- 95 Downloads
The recent interest in wild edible plants is associated with their health benefits, which are mainly due to their richness in antioxidant compounds, particularly phenolics. Nevertheless, some of these compounds are metabolized after ingestion, being transformed into metabolites frequently with lower antioxidant activity. The aim of the present study was to evaluate the influence of the digestive process on the total phenolic contents and antioxidant activity of extracts from four wild edible plants used in the Mediterranean diet (Beta maritima L., Plantago major L., Oxalis pes-caprae L. and Scolymus hispanicus L.). HPLC-DAD analysis revealed that S. hispanicus is characterized by the presence of caffeoylquinic acids, dicaffeoylquinic acids and flavonol derivatives, P. major by high amounts of verbascoside, B. maritima possesses 2,4-dihydroxybenzoic acid, 5-O-caffeoylquinic acid, quercetin derivatives and kaempferol-3-O-rutinoside, and O. pes-caprae extract contains hydroxycinnamic acids and flavone derivatives. Total phenolic contents were determined by Folin–Ciocalteu assay, and antioxidant activity by the ABTS, DPPH, ORAC and FRAP assays. Phenolic contents of P. major and S. hispanicus extracts were not affected by digestion, but they significantly decreased in B. maritima after both phases of digestion process and in O. pes-caprae after the gastric phase. The antioxidant activity results varied with the extract and the method used to evaluate the activity. Results showed that P. major extract has the highest total phenolic contents and antioxidant activity, with considerable values even after digestion, reinforcing the health benefits of this species.
KeywordsBeta maritima L. Gastric digestion Intestinal digestion Plantago major L. Oxalis pes-caprae L. Scolymus hispanicus L
This work received financial support from the European Union (FEDER funds through COMPETE). This work also received financial support from National Funds (FCT/MEC, Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência) through project UID/QUI/50006/2013, co-financed by European Union (FEDER under the Partnership Agreement PT2020), from Programa de Cooperación Interreg V-A España – Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E) and from the project INTERREG - MD.Net: When Brand Meets People. S. Gonçalves acknowledges the financial support from FCT.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human or animal subjects.
- 1.Hadjichambis AC, Paraskeva-Hadjichambi D, Della A, Giusti ME, De Pasquale C, Lenzarini C, Censorii E, González-Tejero MR, Sanchez-Rojas CP, Ramiro-Gutiérrez JM, Skoula M, Johnson C, Sarpaki A, Hmamouchi M, Jorhi S, El-Demerdash M, El-Zayat M, Pieroni A (2008) Wild and semi-domesticated food plant consumption in seven circum-Mediterranean areas. Int J Food Sci Nutr 59:383–414CrossRefGoogle Scholar
- 2.Heinrich M, Leonti M, Nebel S, Peschel W (2005) Local food-nutraceuticals: an example of a multidisciplinary research project on local knowledge. J Physiol Pharmacol 56:5–22Google Scholar
- 11.Zengin G, Mahomoodally MF, Aktumsek A, Ceylan R, Uysala S, Mocan A, Yilmaz MA, Picot-Allain CMN, Ćiriće A, Glamočlija J, Soković M (2018) Functional constituents of six wild edible Silene species: a focus on their phytochemical profiles and bioactive properties. Food Biosci 23:75–82CrossRefGoogle Scholar
- 15.Prior RL, Cao GH (2000) Analysis of botanicals and dietary supplements for antioxidant capacity: a review. J AOAC Int 83:950–956Google Scholar
- 23.Marmouzi I, El Karbane M, El Hamdani M, Kharbach M, Mrabti HN, Alami R, Dahraoui S, El Jemli M, Ouzzif Z, Cherrah Y, Derraji S, El Abbes Faouzi M (2017) Phytochemical and pharmacological variability in Golden Thistle functional parts: comparative study of roots, stems, leaves and flowers. Nat Prod Res 31:2669–2674CrossRefGoogle Scholar
- 47.Siracusa L, Kulišić-Bilušić T, Politeo O, Krause I, Dejanović B, Ruberto G (2011) Phenolic composition and antioxidant activity of aqueous infusions from Capparis spinosa L. and Crithmum maritimum L. before and after submission to a two-step in vitro digestion model. J Agric Food Chem 59:12453–12459CrossRefGoogle Scholar