European Food Research and Technology

, Volume 245, Issue 2, pp 479–487 | Cite as

Antioxidant and angiotensin-I converting enzyme inhibitory peptides from Hippocampus abdominalis

  • Hyun-Soo Kim
  • Jun-Geon Je
  • Bomi Ryu
  • Nalae Kang
  • I. P. Shanura Fernando
  • Thilina U. Jayawardena
  • K. K. Asanka Sanjeewa
  • Jae-Young Oh
  • Tae-Gee Lee
  • You-Jin JeonEmail author
Original Paper


Bioactive peptides isolated from edible marine sources have been used as nutraceuticals and functional foods. The present study focuses on the alcalase hydrolysate of Hippocampus abdominalis for the isolation of antioxidative and angiotensin-I converting enzyme (ACE) inhibitory peptides. Initially, the H. abdominalis alcalase hydrolysate (HA) was separated using ultrafiltration (MWCO = 5 kDa), and have obtained the fraction, HA-III (MW ≤ 5 kDa), with strong bioactivity. This was further separated using gel filtration chromatography (Sephadex G-10) and reverse-phase high-performance liquid chromatography (RP-HPLC). The active Sephadex G-10 fraction was analyzed by the Q-TOF mass spectrometry, nine peptides were identified within the molecular mass range between 757.8 and 990.1 Da. Three peptides, GIIGPSGSP, IGTGIPGIW, and QIGFIW, showed strong ACE inhibitory activity and alkyl radical-scavenging activity. Molecular docking studies revealed that the ACE inhibitory activity of the three active peptides is mainly due to hydrogen bonding interactions and active site interactions between active peptides and ACE. This study demonstrated that the active peptides derived from H. abdominalis can be isolated and the proteolytic hydrolysates of it are potential antioxidant and antihypertensive agents.


Hippocampus abdominalis Sephadex G-10 Q-TOF mass Antioxidant Antihypertensive Peptides 



This research was a part of the Project titled “Development of functional foods using the from hippocampus” (No. 20150343), funded by the Ministry of Oceans and Fisheries, Korea.

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest to declare.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects for evaluation of the bioactivity, but the material comes from seafood of seahorse, a kind of fish.

Supplementary material

217_2018_3179_MOESM1_ESM.docx (163 kb)
Supplementary material 1 (DOCX 162 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Marine Life Sciences, School of Marine Biomedical SciencesJeju National UniversityJejuRepublic of Korea
  2. 2.Department of Marine Bio-food Science, College of Fisheries and Ocean SciencesChonnam National UniversityKwangjuRepublic of Korea
  3. 3.Department of Hotel Cuisine and BakingJeonnam Provincial CollegeDamyangRepublic of Korea

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