Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss)
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Using Western blotting and mass spectrometry we investigated the changes in trout muscle proteins as affected by pre-slaughter nutrient supply practices, including starvation and feed restriction, in comparison to full feeding. A number of fillet quality attributes were also analyzed. During cold storage, fillet firmness was not influenced by the feeding practices, while the initial pH was higher in the treated trout. The early appearance of an alpha-actin 26 kDa-fragment and the detection of enigma protein were both signs of impaired muscle integrity, observed only in the restricted conditions, where water holding capacity was initially lower. The detection of reticulon involved in authophagy and the early finding of apolipoprotein A-I-1 fragments in these restricted samples was consistent with their early degradation in comparison to full ration controls. Since specific protein changes reflected differences in feed management, the use of selected muscle proteins as sensitive indicators of fillet quality is proposed.
KeywordsTrout Fillet quality Mass spectrometry Protein changes Actin
We are grateful to Pierluigi Polese (University of Udine) for providing statistical analysis. We thank Federico Fogolari (University of Udine) for his valuable discussion on proteomics, Elena Saccà and Astrid Fabbro (University of Udine) for their technical support.
The conception and design of the study: TB, MLS, GL; acquisition of data: TB, GC, SB, SS, analyses and interpretation of data: TB, SS; drafting of manuscript: MLS, GL; critical revision: ET; final approval of the version to be published: TB, GC, SB, ET, SS, GL, MLS.
This work was supported by CRITA s.c.a.r.l. project Acquacoltura, Regione Autonoma Friuli Venezia Giulia (L.R. 26/05).
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
Compliance with ethics requirements
This article does not contain any studies with human participants performed by any of the authors. The present study was performed according to the Directive 2010/63/EU of the European Parliament by Scientists accredited by the Italian Ministry of Health in the protection of animals for scientific purposes.
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