PCR analysis of experimental and commercial wines by means of nuclear and chloroplast SSRs
- 138 Downloads
Genetic identification of varieties of grapevines in finished wines is still debated: several papers showed that DNA is extracted and analysed by PCR rather easily from the must, but few barely reproducible results have been presented for DNA extracted in wines after fermentation. This work experimented a method based on CTAB followed by silica purification with NucleoSpin Plant Kit columns to extract DNA from experimental wines of 1 year and commercial wines of 1 or 2 years. The comparison of SSR profiles of wines with those of their grapevine varieties showed that total identity was equal to 47.41% in experimental wines and to 24.31% in commercial wines. In experimental wines, three PCR replicates of three independent DNA preparations are sufficient to capture the alleles of the original grapevine variety, while in the commercial ones this possibility is related to the kind of wine and microsatellite.
KeywordsWine traceability DNA extraction Microsatellites PCR
Authors acknowledge the contribution of the projects FOODINTEGRITY (Grant agreement no. 613688) funded by the European Union’s Seventh Framework Programme for research, technological development and INTENSE-FACCE SURPLUS (Grant agreement no. 652615) funded by European Union’s Horizon 2020 research and innovation programme. Authors are also grateful to University of Parma for technical infrastructures and to “Regione Emilia-Romagna” (Technopole SITEIA-Parma). The authors are also thankful to the University of Parma for providing physical infrastructures in which this work has been performed.
Compliance with ethical standards
Conflict of interest
There are no conflicts to declare.
Compliance with ethics requirements
In this study no animal or human subjects were employed.
- 2.Mirnaghi FS, Mousavi F, Rocha AM, Pawliszyn J (2013) Automated determination of phenolic compounds in wine, berry, and grape samples using 96-blade solid phase microextraction system coupled with liquid chromatography–tandem mass spectrometry. J Chromatogr A 1276:12–19. https://doi.org/10.1016/j.chroma.2012.12.043 CrossRefPubMedGoogle Scholar
- 3.Lehtonen P (1996) Determination of amines and amino acids in wine—a review. Am J Enol Vitic 47:127–133Google Scholar
- 8.Maul E, Töpfer R, Carka F, Cornea V, Crespan M, Dallakyan M, de Andrés Domínguez T, de Lorenzis G, Dejeu L, Goryslavets S, Grando S, Hovannisyan N, Hudcovicova M, Hvarleva T, Ibáñez J, Kiss E, Kocsis L, Lacombe T, Laucou V, Maghradze D, Maletić E, Melyan G, Mihaljević MZ, Muñoz-Organero G, Musayev M, Nebish A, Popescu CF, Regner F, Risovanna V, Ruisa S, Salimov V, Savin G, Schneider A, Stajner N, Ujmajuridze L, Failla O (2015) Identification and characterization of grapevine genetic resources maintained in Eastern European Collections. Vitis 54:5–12Google Scholar
- 18.Recupero M, Garino C, De Paolis A, Cereti E, Coïsson JD, Travaglia F, Arlorio M (2013) A method to check and discover adulteration of nebbiolo-based monovarietal musts: detection of Barbera and Dolcetto cv via SSR analysis coupled with Lab-On-Chip® microcapillary electrophoresis. Food Anal Methods 6: 952–962. https://doi.org/10.1007/s12161-012-9506-3 CrossRefGoogle Scholar
- 19.di Rienzo V, Miazzi MM, Fanelli V, Savino V, Pollastro S, Colucci F, Miccolupo A, Blanco A, Pasqualone A, Montemurro C (2015) An enhanced analytical procedure to discover table grape DNA adulteration in industrial musts. Food Control 60: 124–130. https://doi.org/10.1016/j.foodcont.2015.07.015 CrossRefGoogle Scholar
- 20.Catalano V, Moreno-Sanz P, Lorenzi S, Grando MS (2016) Experimental review of DNA-based methods for wine traceability and development of a single-nucleotide polymorphism (SNP) genotyping assay for quantitative varietal authentication. J Agric Food Chem 64:6969–6984. https://doi.org/10.1021/acs.jafc.6b02560 CrossRefPubMedGoogle Scholar
- 22.Hârta MH, Pamfil D, Pop R, Vicaş S (2011) DNA fingerprinting used for testing some Romanian wine varieties. Bull Univ Agric Sci Vet Med Cluj-Napoca Hortic 68:143–148Google Scholar
- 28.Bowers JE, Dangl GS, Meredith CP (1999) Development and characterization of additional microsatellite DNA markers for grape. Am J Enol Vitic 50:243–246Google Scholar