Preparation of diosgenin from Dioscorea zingiberensis C. H. Wright by stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC)
- 51 Downloads
A novel method of stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC) to extract dioscin of dioscorea zingiberensis. C. H. Wright (DZW) was presented. Compared with the traditional process of direct hydrolysis of DZW, this novel process reduced the non-saponins material entering the hydrolysis, and, as a result, reduced the consumptions of water, acid, and organic solvent significantly, while simultaneously increasing the saponin yield. In addition, the P-HPLC was more efficient, which can not only get high purity, but also the product yield, high speed, simple operation, and it is convenient for industrialized production preparation.
KeywordsDiosgenin Stepwise biocatalysis Foam separation Preparative high-performance liquid chromatography
This research was financially supported by 2017 basic research project Qinghai, China (No. 2017-ZJ-712). The authors thank Prof. Qixiu ZHU of the Key Laboratory of functional organic molecules in Lanzhou University for the assistance in 1HNMR experiments, and Yaling Chi of the Qinghai Normal University for assistance in GC-MS experiments.
Compliance with ethical standards
Conflict of interest
All authors declare that we have no conflict of interest, and the article is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part.
Compliance with ethics requirements
This article does not contain any studies with human or animal subjects.
- 4.Corbiere C, Liagre B, Bianchi A, Bordji K, Dauca M, Netter P, Beneytout JL (2003) Different contribution of apoptosis to the antiproliferative effects of diosgenin and other plant steroids, hecogenin and tigogenin, on human 1547 osteosarcoma cells. Int J Oncol 22(4):899–905Google Scholar
- 7.Zhang XX, Ito Y, Liang JR, Su Q, Zhang YM, Liu JL, Sun WJ (2013) Preparative isolation and purification of five steroid saponins from Dioscorea zingiberensis C.H.Wright by counter-current chromatography coupled with evaporative light scattering detector. J Pharmaceut Biomed 84(10):117–123CrossRefGoogle Scholar
- 12.Gao ZC, Zhang W, Chen YT, Liu HB, Liu L, Lei L (2016) Optimization of foam separation of Dioscin from Dioscorea zingiberensis C. H. Wright by response surface methodology. Food Sci 37(8):26–31Google Scholar
- 14.Xu YW, Han X, Dong DS, Xu LN, Qi Y, Peng JY, Zhan LB (2008) Efficient protocol for purification of diosgenin and two fatty acids from Rhizoma dioscoreae by SFE coupled with high-speed counter-current chromatography and evaporative light scattering detection. J Sep Sci 31(20):3638–3646CrossRefGoogle Scholar