European Food Research and Technology

, Volume 245, Issue 3, pp 581–591 | Cite as

Design of biosystems to provide healthy and safe food—part B: effect on microbial flora and sensory quality of orange juice

  • Behnoush Maherani
  • Mohamed Ali Khlifi
  • Stephane Salmieri
  • Monique LacroixEmail author
Original paper


This study was performed to determine the antifungal activity of plant oil extracts (1% of lemongrass and oregano, 1:1) in combination with organic citrus fruit extract (BIOSECUR F440D®) with a ratio of 1:3 (W/W) against pathogenic molds (Aspergillus niger, Penicillium chrysogenum) and yeast (Saccharomyces cerevisiae). Synthetic commercial preservatives such as sodium benzoate (E211) and potassium sorbate (E202) were used as references. Antifungal formulation-loaded microemulsion was prepared by microfluidization for in situ analysis in orange juice. Antifungal formulation-loaded microemulsion (AFF-MM) led to a total inhibition of targeted fungi during the first days of storage and their populations stayed below detection limit until the end of storage (35 days). AFF-MM presented a higher antifungal activity compared to synthetic preservatives even at a concentration of 10 times lower than optimized concentration. Indeed, microemulsion increased the bioactivity and bioavailability of plant oil/citrus extracts compared to coarse emulsion by increasing the surface area of droplets induced by size decrement. Finally, the sensory analysis showed the panelists acceptance of orange juice in presence of antifungal-loaded microemulsion.


Plant oil extract Organic citrus extract Pathogenic molds and yeast Antifungal activity Microemulsion Sensory analysis 



This work was financially supported by the Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ) (Innov’Action Project #IA 115316) and  Biosecur Lab Inc. Also, the members of RESALA laboratory appreciate the Biosecur Lab for providing Biosecur products.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Behnoush Maherani
    • 1
  • Mohamed Ali Khlifi
    • 1
  • Stephane Salmieri
    • 1
  • Monique Lacroix
    • 1
    Email author
  1. 1.Research Laboratories in Sciences Applied to Food, Canadian Irradiation CenterINRS-Institut Armand-Frappier, Institute of Nutraceutical and Functional FoodsLavalCanada

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