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European Food Research and Technology

, Volume 244, Issue 3, pp 523–534 | Cite as

Investigating the in vitro hypoglycaemic and antioxidant properties of Citrus × clementina Hort. juice

  • Monica R. Loizzo
  • Mariarosaria Leporini
  • Vincenzo Sicari
  • Tiziana Falco
  • Teresa M. Pellicanò
  • Rosa Tundis
Original Paper

Abstract

Citrus × clementina juice obtained from fruits collected in three different areas (flood plain, hill and coastal plain) was investigated for the chemical composition, radical scavenging properties (DPPH and ABTS tests), and α-amylase and α-glucosidase inhibitory activity. Neohesperidin (72.96–116.50 mg/100 mL), hesperidin (55.24–69.52 mg/100 mL) and narirutin (7.21–12.13 mg/100 mL) are the main flavonoids identified by HPLC analyses. In carbohydrate hydrolysing enzymes inhibitory activity tests, samples showed higher potency against α-glucosidase. Juice from hill was the most active with an IC50 value of 77.79 μg/mL. Data on the radical scavenging activity revealed the following trend of potency flood plain > coastal plain > hill. These results could help farmers to select fruits for different industrial purpose such as functional food and matrix to extract nutraceutical products.

Keywords

HPLC phenolic profile Quality parameters Healthy properties PCA 

Abbreviations

ABTS

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

ADA

American Diabetes Association

DPPH

2,2-diphenyl-1-picrylhydrazyl

JNK

C-Jun NH2-terminal kinase

PCA

Principal component analysis

ROS

Reactive oxygen species

T1DM

Type 1 diabetes

T2DM

Type 2 diabetes

TNF-α

Tumour-necrosis factor-α

Notes

Acknowledgements

The authors are grateful to Falco farms for supplying the samples.

Compliance with ethical standards

Funding

The study was not specifically funded.

Conflict of interest

The authors declare that there are no conflicts of interest.

Compliance with ethics requirements

Research does not involve any human participants and/or animal.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Monica R. Loizzo
    • 1
  • Mariarosaria Leporini
    • 1
  • Vincenzo Sicari
    • 1
  • Tiziana Falco
    • 1
  • Teresa M. Pellicanò
    • 2
  • Rosa Tundis
    • 1
  1. 1.Department of Pharmacy, Health Science and NutritionUniversity of CalabriaRendeItaly
  2. 2.Department of AgrariaUniversity “Mediterranea” of Reggio CalabriaReggio CalabriaItaly

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