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Redox-derivatization reaction-based rapid and sensitive determination of nitrite using resonance Rayleigh scattering method

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Abstract

It remains a problem for direct detection of small inorganic nitrite ions using resonance Rayleigh scattering (RRS) method based on the direct dye-binding reaction. In the present study, a redox-derivatization reaction taking only 5 min was introduced prior to nitrite detection. In the redox-derivatization reaction, nitrite ions were reduced by excess iodine ions to generate triiodide ions (I3), which were further derivatized with a cationic dye (basic violet 1, BV1) to form the ion associates of I3-BV1. Therefore, the RRS signal was significantly enhanced, resulting from the increase of particle size and resonance-enhanced scattering effect. The analytical procedure was performed by just mixing nitrite, oxidant, acid, and dye all-in-one, avoiding the tediousness of a multi-step process or the preparation of nanoparticles. The whole detection process including the redox-derivatization reaction was less than 6 min. The reaction conditions such as concentration of hydrochloric acid, potassium iodide, and BV1, reaction time, and temperature were investigated. Under optimum conditions, the concentration of nitrite was linear with an RRS signal of I3-BV1 ion associates at 320 nm in the range of 0.015–1.2 mg/L. The limit of detection (LOD) was calculated to be 3.0 μg/L. The RRS method was applied to the determination of nitrite in real samples such as pork sausage, milk powder, and water with recovery of 95.2–112%. With advantages of rapidness, high sensitivity, and high selectivity, the method indicates potential applicability for detection of nitrite in complex samples. The method also provides an instructive protocol for detection of analytes that generate no/weak RRS enhancement after the direct dye-binding reaction.

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Funding

The authors received financial support by grants from the National Natural Science Foundation of China (21804025), the Guangdong Provincial Natural Science Foundation of China (2018A0303130079), the Youth Innovation Talent Project of Education Department of Guangdong (2017KQNCX105), the Medical Science and Technology Research Foundation of Guangdong (A2018004), and the Innovation and strong School Project of Guangdong Pharmaceutical University.

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Correspondence to Jincan He.

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He, J., Zhong, Z., Tan, S. et al. Redox-derivatization reaction-based rapid and sensitive determination of nitrite using resonance Rayleigh scattering method. Anal Bioanal Chem 412, 1087–1096 (2020). https://doi.org/10.1007/s00216-019-02333-9

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Keywords

  • Nitrite
  • Redox-derivatization reaction
  • Resonance Rayleigh scattering
  • Basic violet 1
  • Colorimetry
  • Spectrophotometric analysis