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A highly sensitive electrochemical biosensor for protein based on a tetrahedral DNA probe, N- and P-co-doped graphene, and rolling circle amplification

Abstract

A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10−14 M for thrombin and a wide linear range from 1 × 10−13 to 1 × 10−7 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21475115), Henan Provincial Science and Technology Innovation Team (C20150026) and the Nanhu Scholars Program of Xinyang Normal University.

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Correspondence to Ke-Jing Huang.

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Wu, Z., Xie, X., Guo, H. et al. A highly sensitive electrochemical biosensor for protein based on a tetrahedral DNA probe, N- and P-co-doped graphene, and rolling circle amplification. Anal Bioanal Chem 412, 915–922 (2020). https://doi.org/10.1007/s00216-019-02314-y

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Keywords

  • Tetrahedral DNA probe
  • N- and P-Co-doped graphene
  • Rolling circle amplification
  • Protein
  • Sensitive determination