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Smartphone colorimetric determination of hydrogen peroxide in real samples based on B, N, and S co-doped carbon dots probe

Abstract

In this paper, we report the use of a smartphone and B, N, and S co-doped carbon dots (BNS-CDs) as a promising peroxidase mimic to quantify hydrogen peroxide (H2O2). The synthesized BNS-CDs exhibited excellent peroxidase-like activity to catalyze the reaction of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with H2O2 to generate a blue oxide product (ox-TMB) with maximum absorption at 652 nm. Steady-state kinetic analysis demonstrated that the BNS-CDs showed much higher affinity than natural horseradish peroxidase (HRP) for H2O2 due to their small size and larger specific surface area. A smartphone colorimetric readout device was employed to record the RGB (red green blue) value of the ox-TMB solution via the Android application Color Grab for quantitative detection. A good linear relationship (R2 = 0.9970) between the H2O2 concentration and |R-Rblank| value was obtained in the range of 3–30 μM with a limit of detection (LOD) of 0.8 μM. The current method was successfully applied to determine H2O2 in mouthwash and milk with recoveries of 92.70–108.30%. The developed assay is a promising portable detection platform for H2O2 with good sensitivity and selectivity, simple operation, fast response, and low cost.

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References

  1. 1.

    Yu J, Ma D, Mei L, Gao Q, Yin W, Zhang X, et al. Peroxidase-like activity of MoS2 nanoflakes with different modifications and their application for H2O2 and glucose detection. J Mater Chem B. 2018;6(3):487–98.

  2. 2.

    Bjerre J, Rousseau C, Marinescu L, Bols M. Artificial enzymes, “chemzymes”: current state and perspectives. Appl Microbiol Biotechnol. 2008;81(1):1–11.

  3. 3.

    Hui W, Erkang W. Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem. 2008;80:2250–4.

  4. 4.

    Mu J, Wang Y, Zhao M, Zhang L. Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun (Camb). 2012;48(19):2540–2.

  5. 5.

    André R, Natálio F, Humanes M, Leppin J, Heinze K, Wever R, et al. V2O5 nanowires with an intrinsic peroxidase-like activity. Adv Funct Mater. 2011;21(3):501–9.

  6. 6.

    Cui M, Zhou J, Zhao Y, Song Q. Facile synthesis of iridium nanoparticles with superior peroxidase-like activity for colorimetric determination of H2O2 and xanthine. Sensors Actuators B Chem. 2017;243:203–10.

  7. 7.

    Hu L, Yuan Y, Zhang L, Zhao J, Majeed S, Xu G. Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection. Anal Chim Acta. 2013;762:83–6.

  8. 8.

    Dehghani Z, Mohammadnejad J, Hosseini M. A new colorimetric assay for amylase based on starch-supported Cu/Au nanocluster peroxidase-like activity. Anal Bioanal Chem. 2019;411(16):3621–9.

  9. 9.

    Liu H, Jiao M, Gu C, Zhang M. Au@CuxOS yolk-shell nanomaterials with porous shells act as a new peroxidase mimic for the colorimetric detection of H2O2. J Alloys Compd. 2018;741:197–204.

  10. 10.

    Yang Z, Cao Y, Li J, Lu M, Jiang Z, Hu X. Smart CuS nanoparticles as peroxidase mimetics for the design of novel label-free chemiluminescent immunoassay. ACS Appl Mater Interfaces. 2016;8(19):12031–8.

  11. 11.

    Song W, Zhao B, Wang C, Ozaki Y, Lu X. Functional nanomaterials with unique enzyme-like characteristics for sensing applications. J Mater Chem B. 2019;7(6):850–75.

  12. 12.

    Zhang Y, Xu C, Li B, Li Y. In situ growth of positively-charged gold nanoparticles on single-walled carbon nanotubes as a highly active peroxidase mimetic and its application in biosensing. Biosens Bioelectron. 2013;43:205–10.

  13. 13.

    Lin L, Song X, Chen Y, Rong M, Zhao T, Wang Y, et al. Intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots and their application in the colorimetric detection of H2O2 and glucose. Anal Chim Acta. 2015;869:89–95.

  14. 14.

    Sun W, Ju X, Zhang Y, Sun X, Li G, Sun Z. Application of carboxyl functionalized graphene oxide as mimetic peroxidase for sensitive voltammetric detection of H2O2 with 3,3′,5,5′-tetramethylbenzidine. Electrochem Commun. 2013;26:113–6.

  15. 15.

    Molaei MJ. A review on nanostructured carbon quantum dots and their applications in biotechnology, sensors, and chemiluminescence. Talanta. 2019;196:456–78.

  16. 16.

    Mehta VN, Chettiar SS, Bhamore JR, Kailasa SK, Patel RM. Green synthetic approach for synthesis of fluorescent carbon dots for lisinopril drug delivery system and their confirmations in the cells. J Fluoresc. 2017;27(1):111–24.

  17. 17.

    Han Y, Huang H, Zhang H, Liu Y, Han X, Liu R, et al. Carbon quantum dots with Photoenhanced hydrogen-bond catalytic activity in Aldol condensations. ACS Catal. 2014;4(3):781–7.

  18. 18.

    Mirtchev P, Henderson EJ, Soheilnia N, Yip CM, Ozin GA. Solution phase synthesis of carbon quantum dots as sensitizers for nanocrystalline TiO2solar cells. J Mater Chem. 2012;22(4):1265–9.

  19. 19.

    Liu J, Wang L, Bao H. A novel fluorescent probe for ascorbic acid based on seed-mediated growth of silver nanoparticles quenching of carbon dots fluorescence. Anal Bioanal Chem. 2019;411(4):877–83.

  20. 20.

    Chandra S, Singh VK, Yadav PK, Bano D, Kumar V, Pandey VK, et al. Mustard seeds derived fluorescent carbon quantum dots and their peroxidase-like activity for colorimetric detection of H2O2 and ascorbic acid in a real sample. Anal Chim Acta. 2019;1054:145–56.

  21. 21.

    Shi W, Wang Q, Long Y, Cheng Z, Chen S, Zheng H, et al. Carbon nanodots as peroxidase mimetics and their applications to glucose detection. Chem Commun. 2011;47:6695–7.

  22. 22.

    Wu D, Deng X, Huang X, Wang K, Liu Q. Low-cost preparation of photoluminescent carbon nanodots and application as peroxidase mimetics in colorimetric detection of H2O2 and glucose. J Nanosci Nanotechnol. 2013;13(10):6611–6.

  23. 23.

    Zhong Q, Chen Y, Su A, Wang Y. Synthesis of catalytically active carbon quantum dots and its application for colorimetric detection of glutathione. Sensors Actuators B Chem. 2018;273:1098–102.

  24. 24.

    Bano D, Kumar V, Singh VK, Chandra S, Singh DK, Yadav PK, et al. A facile and simple strategy for the synthesis of label free carbon quantum dots from the latex of Euphorbia milii and its peroxidase-mimic activity for the naked eye detection of glutathione in a human blood serum. ACS Sustain Chem Eng. 2018;7(2):1923–32.

  25. 25.

    Tang M, Zhu B, Wang Y, Wu H, Chai F, Qu F, et al. Nitrogen- and sulfur-doped carbon dots as peroxidase mimetics: colorimetric determination of hydrogen peroxide and glutathione, and fluorimetric determination of lead(II). Microchim Acta. 2019;186(9):604.

  26. 26.

    Jiang D, Du X, Liu Q, Zhou L, Qian J, Wang K. One-step thermal-treatment route to fabricate well-dispersed ZnO nanocrystals on nitrogen-doped graphene for enhanced electrochemiluminescence and ultrasensitive detection of pentachlorophenol. ACS Appl Mater Interfaces. 2015;7(5):3093–100.

  27. 27.

    Lee DG, Yang C-M, Kim B-H. Enhanced electrochemical properties of boron functional groups on porous carbon nanofiber/MnO 2 materials. J Electroanal Chem. 2017;788:192–7.

  28. 28.

    Singh VK, Yadav PK, Chandra S, Bano D, Talat M, Hasan SH. Peroxidase mimetic activity of fluorescent NS-carbon quantum dots and their application in colorimetric detection of H2O2 and glutathione in human blood serum. J Mater Chem B. 2018;6(32):5256–68.

  29. 29.

    Li F, Hu Y, Li Z, Liu J, Guo L, He J. Three-dimensional microfluidic paper-based device for multiplexed colorimetric detection of six metal ions combined with use of a smartphone. Anal Bioanal Chem. 2019;411:6497–508.

  30. 30.

    Yang M, Zhang Y, Cui M, Tian Y, Zhang S, Peng K, et al. A smartphone-based quantitative detection platform of mycotoxins based on multiple-color upconversion nanoparticles. Nanoscale. 2018;10(33):15865–74.

  31. 31.

    Spyrou EM, Kalogianni DP, Tragoulias SS, Ioannou PC, Christopoulos TK. Digital camera and smartphone as detectors in paper-based chemiluminometric genotyping of single nucleotide polymorphisms. Anal Bioanal Chem. 2016;408(26):7393–402.

  32. 32.

    Roda A, Michelini E, Cevenini L, Calabria D, Calabretta MM, Simoni P. Integrating biochemiluminescence detection on smartphones: mobile chemistry platform for point-of-need analysis. Anal Chem. 2014;86(15):7299–304.

  33. 33.

    Peng B, Zhou J, Xu J, Fan M, Ma Y, Zhou M, et al. A smartphone-based colorimetry after dispersive liquid–liquid microextraction for rapid quantification of calcium in water and food samples. Microchem J. 2019. https://doi.org/10.1016/j.microc.2019.104072.

  34. 34.

    Liu Y, Duan W, Song W, Liu J, Ren C, Wu J, et al. Red Emission B, N, S-co-doped carbon dots for colorimetric and fluorescent dual mode detection of Fe(3+) ions in complex biological fluids and living cells. ACS Appl Mater Interfaces. 2017;9(14):12663–72.

  35. 35.

    Feng J, Ju Y, Liu J, Zhang H, Chen X. Polyethyleneimine-templated copper nanoclusters via ascorbic acid reduction approach as ferric ion sensor. Anal Chim Acta. 2015;854:153–60.

  36. 36.

    Color Grab. Loomatix Ltd. URL http://www.loomatix.com/. Accessed 23 July 2019.

  37. 37.

    Ravindranath R, Periasamy AP, Roy P, Chen YW, Chang HT. Smart app-based on-field colorimetric quantification of mercury via analyte-induced enhancement of the photocatalytic activity of TiO2-Au nanospheres. Anal Bioanal Chem. 2018;410(18):4555–64.

  38. 38.

    Pham NA, Morrison A, Schwock J, Aviel-Ronen S, Iakovlev V, Tsao MS, et al. Quantitative image analysis of immunohistochemical stains using a CMYK color model. Diagn Pathol. 2007;2(1):8–18.

  39. 39.

    Machado JMD, Soares RRG, Chu V, Conde JP. Multiplexed capillary microfluidic immunoassay with smartphone data acquisition for parallel mycotoxin detection. Biosens Bioelectron. 2018;99:40–6.

  40. 40.

    Mohamed AA, Shalaby AA. Digital imaging devices as sensors for iron determination. Food Chem. 2019;274:360–7.

  41. 41.

    Reckmeier CJ, Wang Y, Zboril R, Rogach AL. Influence of doping and temperature on solvatochromic shifts in optical spectra of carbon dots. J Phys Chem C. 2016;120(19):10591–604.

  42. 42.

    Huang S, Yang E, Yao J, Liu Y, Xiao Q. Red emission nitrogen, boron, sulfur co-doped carbon dots for “on-off-on” fluorescent mode detection of Ag(+) ions and l-cysteine in complex biological fluids and living cells. Anal Chim Acta. 2018;1035:192–202.

  43. 43.

    Tian T, He Y, Ge Y, Song G. One-pot synthesis of boron and nitrogen co-doped carbon dots as the fluorescence probe for dopamine based on the redox reaction between Cr(VI) and dopamine. Sensors Actuators B Chem. 2017;240:1265–71.

  44. 44.

    Zhang JW, Zhang HT, Du ZY, Wang X, Yu SH, Jiang HL. Water-stable metal-organic frameworks with intrinsic peroxidase-like catalytic activity as a colorimetric biosensing platform. Chem Commun (Camb). 2014;50(9):1092–4.

  45. 45.

    Huang Y, Cheng Z. Simple and green synthesis of boron-, sulfur-, and nitrogen-co-doped carbon dots as fluorescent probe for selective and sensitive detection of sunset yellow. Nano. 2017;12(10):1750123–34.

  46. 46.

    Long Y, Wang X, Shen D, Zheng H. Detection of glucose based on the peroxidase-like activity of reduced state carbon dots. Talanta. 2016;159:122–6.

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Funding

This study received financial support from the National Key Research and Development Program of China (2018YFC1603700) and the National Natural Science Foundation of China (21767026, 21862019).

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Correspondence to Bo Peng or Yanjun Fang.

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Peng, B., Xu, J., Fan, M. et al. Smartphone colorimetric determination of hydrogen peroxide in real samples based on B, N, and S co-doped carbon dots probe. Anal Bioanal Chem 412, 861–870 (2020). https://doi.org/10.1007/s00216-019-02284-1

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Keywords

  • Smartphone
  • RGB
  • Carbon dots
  • Peroxidase
  • Hydrogen peroxide
  • TMB