Advertisement

Analytical and Bioanalytical Chemistry

, Volume 411, Issue 29, pp 7899–7906 | Cite as

Development of a filtration-based SERS mapping platform for specific screening of Salmonella enterica serovar Enteritidis

  • Siyue Gao
  • Lili HeEmail author
Research Paper

Abstract

The presence of Salmonella in natural freshwater and drinking water is a leading cause of intestinal illness all over the world; thus, the detection of Salmonella in water is of great importance to public health. The objective of this study is to develop a rapid screening method for the detection of Salmonella enterica serovar Enteritidis in water involving surface-enhanced Raman spectroscopy (SERS), aptamers, and filtration. SERS offers a great alternative to traditional methods of pathogen detection, with a simplified detection assay and shortened detection time. The specific capturing and labeling of Salmonella Enteritidis are realized by a specific single-stranded DNA aptamer, which is modified with an additional chain of adenine and fluorescein (FAM) and used as presence/absence indicator of Salmonella Enteritidis. By incorporating a vacuum filtration system, bacterial cells recognized by the specific aptamer are concentrated onto a membrane. With additional filtration of gold nanoparticles, the aptamer signals were captured and used to construct a SERS mapping indicating the presence and absence of target bacterial strains with potential quantitative capability. The specificity of the method was validated by using other strains of bacteria such as Escherichia coli and Listeria monocytogenes. The sensitivity of the method goes down to 103 CFU/mL for 1 mL of sample with a total detection and analyzing time within 3 h. This study demonstrates the capability of the filtration-based SERS platform for detecting Salmonella Enteritidis in various aqueous matrices such as distilled water and rinsing water from fresh produce with high selectivity and sensitivity.

Graphical abstract

Keywords

Salmonella Enteritidis Filtration Aptamers Surface-enhanced Raman spectroscopy 

Notes

Funding information

This work is funded by USDA-NIFA 2015-67021-22993 and USDA-NIFA hatch (MAS00491).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2204_MOESM1_ESM.pdf (337 kb)
ESM 1 (PDF 336 kb)

References

  1. 1.
    Levantesi C, Bonadonna L, Briancesco R, Grohmann E, Toze S, Tandoi V. Salmonella in surface and drinking water: occurrence and water-mediated transmission. Food Res Int. 2012;45:587–602.CrossRefGoogle Scholar
  2. 2.
    Beuchat LR. Pathogenic microorganisms associated with fresh produce. J Food Prot. 1996.  https://doi.org/10.4315/0362-028X-59.2.204.CrossRefGoogle Scholar
  3. 3.
    Andino A, Hanning I. Salmonella enterica: survival, colonization, and virulence differences among serovars. Sci World J. 2015.  https://doi.org/10.1155/2015/520179.CrossRefGoogle Scholar
  4. 4.
    Bogosian G, Bourneuf EV. A matter of bacterial life and death. EMBO Rep. 2001;2:770–4.CrossRefGoogle Scholar
  5. 5.
    Su X, Chen X, Hu J, Shen C, Ding L. Exploring the potential environmental functions of viable but non-culturable bacteria. World J Microbiol Biotechnol. 2013;29:2213–8.CrossRefGoogle Scholar
  6. 6.
    Malorny B, Paccassoni E, Fach P, Bunge C, Martin A, Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl Environ Microbiol. 2004;70:7046–52.CrossRefGoogle Scholar
  7. 7.
    Josephson KL, Gerba CP, Pepper IL. Polymerase chain reaction detection of nonviable bacterial pathogens. Appl Environ Microbiol. 1993;59:3513–5.PubMedPubMedCentralGoogle Scholar
  8. 8.
    Kong RYC, Lee SKY, Law TWF, Law SHW, Wu RSS. Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR. Water Res. 2002;36:2802–12.CrossRefGoogle Scholar
  9. 9.
    Braker G, Fesefeldt A, Witzel K-P. Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Appl Environ Microbiol. 1998;64:3769–75.PubMedPubMedCentralGoogle Scholar
  10. 10.
    Kumar BK, Raghunath P, Devegowda D, Deekshit VK, Venugopal MN, Karunasagar I, et al. Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood. Int J Food Microbiol. 2011;145:244–9.CrossRefGoogle Scholar
  11. 11.
    Cho I-H, Irudayaraj J. In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection. Int J Food Microbiol. 2013;164:70–5.CrossRefGoogle Scholar
  12. 12.
    Schloter M, Aßmus B, Hartmann A. The use of immunological methods to detect and identify bacteria in the environment. Biotechnol Adv. 1995;13:75–90.CrossRefGoogle Scholar
  13. 13.
    Tripathi SM, Bock WJ, Mikulic P, Chinnappan R, Ng A, Tolba M, et al. Long period grating based biosensor for the detection of Escherichia coli bacteria. Biosens Bioelectron. 2012;35:308–12.CrossRefGoogle Scholar
  14. 14.
    Ivnitski D, Abdel-Hamid I, Atanasov P, Wilkins E, Stricker S. Application of electrochemical biosensors for detection of food pathogenic bacteria. Electroanalysis. 2000.  https://doi.org/10.1002/(SICI)1521-4109(20000301)12:5<317::AID-ELAN317>3.0.CO;2-A.
  15. 15.
    Huang Y, Dong X, Liu Y, Li L-J, Chen P. Graphene-based biosensors for detection of bacteria and their metabolic activities. J Mater Chem. 2011.  https://doi.org/10.1039/c1jm11436k.CrossRefGoogle Scholar
  16. 16.
    Lazcka O, Del Campo FJ, Muñoz FX. Pathogen detection: a perspective of traditional methods and biosensors. Biosens Bioelectron. 2007;22:1205–17.CrossRefGoogle Scholar
  17. 17.
    Brewster JD, Mazenko RS. Filtration capture and immunoelectrochemical detection for rapid assay of Escherichia coli O157:H7. J Immunol Methods. 1998.  https://doi.org/10.1016/S0022-1759(97)00161-0.CrossRefGoogle Scholar
  18. 18.
    Wolffs PFG, Glencross K, Thibaudeau R, Griffiths MW. Direct quantitation and detection of salmonellae in biological samples without enrichment, Using Two-Step Filtration and Real-Time PCR. Appl Environ Microbiol. 2006;72:3896–900.CrossRefGoogle Scholar
  19. 19.
    Lee H, Yoon TJ, Weissleder R. Ultrasensitive detection of bacteria using core-shell nanoparticles and an NMR-filter system. Angew Chem Int Ed. 2009.  https://doi.org/10.1002/anie.200901791.CrossRefGoogle Scholar
  20. 20.
    Gao S, Pearson B, He L. Mapping bacteria on filter membranes, an innovative SERS approach. J Microbiol Methods. 2018.  https://doi.org/10.1016/j.mimet.2018.03.005.CrossRefGoogle Scholar
  21. 21.
    Ohk SH, Koo OK, Sen T, Yamamoto CM, Bhunia AK. Antibody–aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J Appl Microbiol. 2010;109:808–17.CrossRefGoogle Scholar
  22. 22.
    Urmann K, Walter JG, Scheper T, Segal E. Label-free optical biosensors based on aptamer-functionalized porous silicon scaffolds. Anal Chem. 2015.  https://doi.org/10.1021/ac504487g.CrossRefGoogle Scholar
  23. 23.
    Maeng J-S, Kim N, Kim C-T, Han SR, Lee YJ, Lee S-W, et al. Rapid detection of food pathogens using RNA aptamers-immobilized slide. J Nanosci Nanotechnol. 2012.  https://doi.org/10.1166/jnn.2012.6369.CrossRefGoogle Scholar
  24. 24.
    Duan N, Ding X, He L, Wu S, Wei Y, Wang Z. Selection, identification and application of a DNA aptamer against Listeria monocytogenes. Food Control. 2013.  https://doi.org/10.1016/j.foodcont.2013.03.011.CrossRefGoogle Scholar
  25. 25.
    Abbaspour A, Norouz-Sarvestani F, Noori A, Soltani N. Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureus. Biosens Bioelectron. 2015;68:149–55.CrossRefGoogle Scholar
  26. 26.
    Ma X, Jiang Y, Jia F, Yu Y, Chen J, Wang Z. An aptamer-based electrochemical biosensor for the detection of Salmonella. J Microbiol Methods. 2014;98:94–8.CrossRefGoogle Scholar
  27. 27.
    Muhammad-Tahir Z, Alocilja EC. A conductometric biosensor for biosecurity. Biosens Bioelectron. 2003.  https://doi.org/10.1016/S0956-5663(03)00020-4.CrossRefGoogle Scholar
  28. 28.
    Mirsky VM, Riepl M, Wolfbeis OS. Capacitive monitoring of protein immobilization and antigen-antibody reactions on monomolecular alkylthiol films on gold electrodes. Biosens Bioelectron. 1997.  https://doi.org/10.1016/S0956-5663(97)00053-5.CrossRefGoogle Scholar
  29. 29.
    Cao X, Li S, Chen L, et al. Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus. Nucleic Acids Res. 2009.  https://doi.org/10.1093/nar/gkp489.CrossRefGoogle Scholar
  30. 30.
    Duan N, Wu S, Zhu C, Ma X, Wang Z, Yu Y, et al. Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus. Anal Chim Acta. 2012.  https://doi.org/10.1016/j.aca.2012.02.011.CrossRefGoogle Scholar
  31. 31.
    Zhang H, Ma X, Liu Y, Duan N, Wu S, Wang Z, et al. Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus. Biosens Bioelectron. 2015;74:872–7.CrossRefGoogle Scholar
  32. 32.
    Gracie K, Correa E, Mabbott S, Dougan JA, Graham D, Goodacre R, et al. Simultaneous detection and quantification of three bacterial meningitis pathogens by SERS. Chem Sci. 2014;5:1030–40.CrossRefGoogle Scholar
  33. 33.
    Kolovskaya OS, Savitskaya AG, Zamay TN, et al. Development of bacteriostatic DNA aptamers for salmonella. J Med Chem. 2013.  https://doi.org/10.1021/jm301856j.CrossRefGoogle Scholar
  34. 34.
    Labib M, Zamay AS, Kolovskaya OS, Reshetneva IT, Zamay GS, Kibbee RJ, et al. Aptamer-based impedimetric sensor for bacterial typing. Anal Chem. 2012;84:8114–7.CrossRefGoogle Scholar
  35. 35.
    Wang F, Liu B, Huang PJJ, Liu J. Rationally designed nucleobase and nucleotide coordinated nanoparticles for selective DNA adsorption and detection. Anal Chem. 2013.  https://doi.org/10.1021/ac4033627.CrossRefGoogle Scholar
  36. 36.
    Gracie K, Moores M, Smith WE, Harding K, Girolami M, Graham D, et al. Preferential attachment of specific fluorescent dyes and dye labeled DNA sequences in a surface enhanced Raman scattering multiplex. Anal Chem. 2016;88:1147–53.CrossRefGoogle Scholar
  37. 37.
    Urmann K, Arshavsky-Graham S, Walter JG, Scheper T, Segal E. Whole-cell detection of live: lactobacillus acidophilus on aptamer-decorated porous silicon biosensors. Analyst. 2016.  https://doi.org/10.1039/c6an00810k.CrossRefGoogle Scholar
  38. 38.
    Mayer G, Ahmed MSL, Dolf A, Endl E, Knolle PA, Famulok M. Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures. Nat Protoc. 2010.  https://doi.org/10.1038/nprot.2010.163.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food ScienceUniversity of Massachusetts AmherstAmherstUSA

Personalised recommendations