Advertisement

Leek-derived codoped carbon dots as efficient fluorescent probes for dichlorvos sensitive detection and cell multicolor imaging

  • Yuefang HuEmail author
  • Jinfang LiEmail author
  • Xuefeng Li
Research Paper
  • 61 Downloads

Abstract

A biomass nitrogen and sulfur codoped carbon dots (NS-Cdots) was prepared by a simple and clean hydrothermal method using leek, and was employed as efficient fluorescent probes for sensitive detection of organophosphorus pesticides (OPs). The leek-derived NS-Cdots emitted blue fluorescence, but was quenched by H2O2. Due to acetylcholinesterase/choline oxidase–based cascade enzymatic reaction that produces H2O2 and the inhibition effect of OPs on acetylcholinesterase activity, a NS-Cdots-based fluorescence “off-on” method to detect OPs-dichlorvos (DDVP) was developed. More sensitivity and wider linear detection range were achieved from 1.0 × 10−9 to 1.0 × 10−3 M (limit of detection = 5.0 × 10−10 M). This developed method was applied to the detection of DDVP in Chinese cabbage successfully. The average recoveries were in the range of 96.0~104.0% with a relative standard deviation of less than 3.3%. In addition, the NS-Cdots fluorescent probes were also employed successfully in multicolor imaging of living cells, manifesting that the NS-Cdots fluorescent probes have great application potential in agricultural and biomedical fields.

Graphical Abstract

Keywords

Codoped carbon dots Organophosphorus pesticides Fluorescence detection Cells multicolor imaging 

Notes

Funding information

This study was funded by the Natural Science Foundation of Guangxi Province (No. 2017GXNSFAA198274)

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards and informed consent

This research was approved by the Hezhou University Ethic Committee and all experiments were performed in accordance with the Guideline for Experimentation of Hezhou University.

Supplementary material

216_2019_2192_MOESM1_ESM.pdf (702 kb)
ESM 1 (PDF 702 kb)

References

  1. 1.
    Jokanović M. Neurotoxic effects of organophosphorus pesticides and possible association with neurodegenerative diseases in man: a review. Toxicology. 2018;410:125–31.CrossRefGoogle Scholar
  2. 2.
    Kesani S, Malik A. Front Cover: Sol-gel niobia sorbent with a positively charged octadecyl ligand providing enhanced enrichment of nucleotides and organophosphorus pesticides in capillary microextraction for online HPLC analysis. J Sep Sci. 2018;41(7):1663–73.CrossRefGoogle Scholar
  3. 3.
    Zhang X, Tang Q, Wang Q, Qiao X, Xu Z. Study of a molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography for simultaneous determination of trace trichlorfon and monocrotophos residues in vegetables. J Sci Food Agric. 2014;94(7):1409–15.CrossRefGoogle Scholar
  4. 4.
    Jafari MT, Saraji M, Kermani M. Sol-gel electrospinning preparation of hybrid carbon silica nanofibers for extracting organophosphorus pesticides prior to analyzing them by gas chromatography-ion mobility spectrometry. J Chromatogr A. 2018;1558:1–13.CrossRefGoogle Scholar
  5. 5.
    Li D, He M, Chen B, Hu B. Metal organic frameworks-derived magnetic nanoporous carbon for preconcentration of organophosphorus pesticides from fruit samples followed by gas chromatography-flame photometric detection. J Chromatogr A. 2018;1583:19–27.CrossRefGoogle Scholar
  6. 6.
    Akbarzade S, Chamsaz M, Rounaghi GH, Ghorbani M. Zero valent Fe-reduced graphene oxide quantum dots as a novel magnetic dispersive solid phase microextraction sorbent for extraction of organophosphorus pesticides in real water and fruit juice samples prior to analysis by gas chromatography-mass spectrometry. Anal Bioanal Chem. 2018;410(2):429–39.CrossRefGoogle Scholar
  7. 7.
    Cacho JI, Campillo N, Viñas P, Hernández-Córdoba M. In situ ionic liquid dispersive liquid-liquid microextraction coupled to gas chromatography-mass spectrometry for the determination of organophosphorus pesticides. J Chromatogr A. 2018;1559:95–101.CrossRefGoogle Scholar
  8. 8.
    Choi JR, Yong KW, Choi JY, Cowie AC. Progress in Molecularly Imprinted Polymers for Biomedical Applications. Comb Chem High Throughput Screen. 2019.  https://doi.org/10.2174/1386207322666190325115526.CrossRefGoogle Scholar
  9. 9.
    Boulanouar S, Mezzache S, Combès A, Pichon V. Molecularly imprinted polymers for the determination of organophosphorus pesticides in complex samples. Talanta. 2018;176:465–78.CrossRefGoogle Scholar
  10. 10.
    Boulanouar S, Combès A, Mezzache S, et al. Synthesis and application of molecularly imprinted silica for the selective extraction of some polar organophosphorus pesticides from almond oil. Anal Chim Acta. 2018;1018:35–44.CrossRefGoogle Scholar
  11. 11.
    Meng X, Schultz CW, Cui C, Li X, Yu HZ. On-site chip-based colorimetric quantitation of organophosphorus pesticides using an office scanner. Sensors Actuators B Chem. 2015;215:577–83.CrossRefGoogle Scholar
  12. 12.
    Mostafalou S, Abdollahi M. The link of organophosphorus pesticides with neurodegenerative and neurodevelopmental diseases based on evidence and mechanisms. Toxicology. 2018;409:44–52.CrossRefGoogle Scholar
  13. 13.
    Yan X, Song Y, Zhu C, Li H, Du D, Su X, et al. MnO2 Nanosheet-carbon dots sensing platform for sensitive detection of organophosphorus pesticides. Anal Chem. 2018;90:2618–24.CrossRefGoogle Scholar
  14. 14.
    Xie H, Bei F, Hou J, Ai S. A highly sensitive dual-signaling assay via inner filter effect between g-C3N4 and gold nanoparticles for organophosphorus pesticides. Sensors Actuators B Chem. 2018;255:2232–9.CrossRefGoogle Scholar
  15. 15.
    Zhao Y, Yang M, Fu Q, Ouyang H, Wen W, Song Y, et al. A Nanozyme- and ambient light-based smartphone platform for simultaneous detection of dual biomarkers from exposure to organophosphorus pesticides. Anal Chem. 2018;90:7391–8.CrossRefGoogle Scholar
  16. 16.
    Yang M, Zhao Y, Wang L, Paulsen M, Simpson CD, Liu F, et al. Simultaneous detection of dual biomarkers from humans exposed to organophosphorus pesticides by combination of immunochromatographic test strip and Ellman assay. Biosens Bioelectron. 2018;104:39–44.CrossRefGoogle Scholar
  17. 17.
    Wu F, Yang M, Zhang H, Zhu S, Zhu X, Wang K. Facile synthesis of sulfur-doped carbon quantum dots from vitamin B1 for highly selective detection of Fe3+ ion. Opt Mater. 2018;77:258–63.CrossRefGoogle Scholar
  18. 18.
    Angamuthu R, Palanisamy P, Vasudevan V, Nagarajan S, Rajendran R, Vairamuthu R. Quick synthesis of 2-propanol derived fluorescent carbon dots for bioimaging applications. Opt Mater. 2018;78:477–83.CrossRefGoogle Scholar
  19. 19.
    Chen Q, Zhu P, Xiong J, Gao L, Tan K. A sensitive and selective triple-channel optical assay based on red-emissive carbon dots for the determination of PFOS. Microchem J. 2019;145:388–96.CrossRefGoogle Scholar
  20. 20.
    Shahbazi N, Zare-Dorabei R. A novel “off-on” fluorescence nanosensor for sensitive determination of sulfide ions based on carbon quantum dots and gold nanoparticles: central composite design optimization. Microchem J. 2019;145:996–1002.CrossRefGoogle Scholar
  21. 21.
    Liao S, Huang X, Yang H, Chen X. Nitrogen-doped carbon quantum dots as a fluorescent probe to detect copper ions, glutathione, and intracellular pH. Anal Bioanal Chem. 2018;410(29):7701–10.CrossRefGoogle Scholar
  22. 22.
    Chen X, Gong F, Cao Z, Zou W, Gu T. Highly cysteine-selective fluorescent nanoprobes based on ultrabright and directly synthesized carbon quantum dots. Anal Bioanal Chem. 2018;410(12):2961–70.CrossRefGoogle Scholar
  23. 23.
    Hu Y, Lin L, Li J, Ye J. P, N Codoped carbon dots as an efficient “off-on” fluorescent probe for lipoic acid detection and its cellular dual-color imaging. Anal Bioanal Chem. 2019;411(16):3603–12.CrossRefGoogle Scholar
  24. 24.
    Xue M, Zhao J, Zhan Z, Zhao S, Lan C, Ye F, et al. Dual functionalized natural biomass carbon dots from lychee exocarp for cancer cell targetable near-infrared fluorescence imaging and photodynamic therapy. Nanoscale. 2018;10(38):18124–30.CrossRefGoogle Scholar
  25. 25.
    Meng W, Bai X, Wang B, Liu Z, Lu S, Yang B. Biomass-derived carbon dots and their applications. Energy Environ Mater. 2019;0:1–21.Google Scholar
  26. 26.
    Hu Y, Zhang L, Li X, Liu R, Lin L, Zhao S. Green preparation of S and N co-doped carbon dots from water chestnut and onion as well as their use as an off–on pluorescent probe for the quantification and imaging of coenzyme A. ACS Sustain Chem Eng. 2017;5:4992–5000.CrossRefGoogle Scholar
  27. 27.
    Hu Y, Chen Z, Lai F, Li J. Biomass-codoped carbon dots: efficient fluorescent probes for isocarbophos ultrasensitive detection and for living cells dual-color imaging. J Mater Sci. 2019;54(11):8627–39.CrossRefGoogle Scholar
  28. 28.
    Hu Y, Zhao J, Li X, Zhao S. Biomass-based quantum dots co-doped with sulfur and nitrogen for highly sensitive detection of thrombin and inhibitor. New J Chem. 2019;43:11510–6.CrossRefGoogle Scholar
  29. 29.
    Shi L, Li Y, Li X, Zhao B, Wen X, Zhang G, et al. Controllable synthesis of green and blue fluorescent carbon nanodots for pH and Cu2+ sensing in living cells. Biosens Bioelectron. 2016;77:598–602.CrossRefGoogle Scholar
  30. 30.
    Chang J, Li H, Hou T, Li F. based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity. Biosens Bioelectron. 2016;86:971–7.CrossRefGoogle Scholar
  31. 31.
    Shi B, Su Y, Zhang L, Liu R, Huang M, Zhao S. Nitrogen-rich functional groups carbon nanoparticles based fluorescent pH sensor with broad-range responding for environmental and live cells applications. Biosens Bioelectron. 2016;82:233–9.CrossRefGoogle Scholar
  32. 32.
    Lu W, Gong X, Nan M, Liu Y, Shuang S, Dong C. Comparative study for N and S doped carbon dots: synthesis, characterization and applications for Fe3+ probe and cellular imaging. Anal Chim Acta. 2015;898:116–27.CrossRefGoogle Scholar
  33. 33.
    Sun D, Ban R, Zhang PH, Zhang J, Zhu J. Hair fiber as a precursor for synthesizing of sulfur-and nitrogen-co-doped carbon dots with tunable luminescence properties. Carbon. 2013;64:424–34.CrossRefGoogle Scholar
  34. 34.
    Song Z, Quan F, Xu Y, Liu M, Cui L, Liu J. Multifunctional N, S co-doped carbon quantum dots with pH- and thermo-dependent switchable fluorescent properties and highly selective detection of glutathione. Carbon. 2016;104:169–78.CrossRefGoogle Scholar
  35. 35.
    Teng X, Ma C, Ge C, Yan M, Yang J, Zhang Y, et al. Green synthesis of nitrogen-doped carbon dots from konjac flour with “off–on” fluorescence by Fe 3+ and L-lysine for bioimaging. J Chem Mater B. 2014;2(29):4631–9.CrossRefGoogle Scholar
  36. 36.
    Zhou J, Sheng Z, Han H, Zou M, Li C. Facile synthesis of fluorescent carbon dots using watermelon peel as a carbon source. Mater Lett. 2012;66(1):222–4.CrossRefGoogle Scholar
  37. 37.
    Lu W, Qin X, Asiri AM, Al-Youbi AQ, Sun X. Green synthesis of carbon nanodots as an effective fluorescent probe for sensitive and selective detection of mercury (II) ions. J Nanopart Res. 2013;15(1):1344–50.CrossRefGoogle Scholar
  38. 38.
    Li L, Yu B, You T. Nitrogen and sulfur co-doped carbon dots for highly selective and sensitive detection of Hg (II) ions. Biosens Bioelectron. 2015;74:263–9.CrossRefGoogle Scholar
  39. 39.
    Wang F, Liu X, Lu CH, Willner I. Cysteine-mediated aggregation of Au nanoparticles: the development of a H2O2 sensor and oxidase-based biosensors. ACS Nano. 2013;7(8):7278–86.CrossRefGoogle Scholar
  40. 40.
    Zhao T, Wang Q, Li J, Qiao X, Xu Z. Study on an electrochromatography method based on organic–inorganic hybrid molecularly imprinted monolith for determination of trace trichlorfon in vegetables. J Sci Food Agric. 2014;94(10):1974–80.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Materials and Chemical EngineeringHezhou UniversityHezhouChina
  2. 2.College of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGuilinChina

Personalised recommendations