Efficient AuPd@GO-based electrochemical nanoprobe for sensitive detection of histone acetylase activity and its inhibitor

  • Qiong Liu
  • Linfei Yang
  • Yuqi SheEmail author
  • Yufang HuEmail author
Research Paper


Histone acetylase (HAT p300), which has aroused great concern in fundamental research and clinical applications, serves as one class of significant tumor markers. In our work, a sensitive electrochemical immunoassay for testing HAT p300 based on both graphene-assisted supported AuPd nanomaterial (AuPd@GO composite) and a typical amperometric i-t technique with fast response is developed favorably. The AuPd@GO-based sensing mechanisms are distributed as follows: the HAT p300 derived acetylation reaction occurs at the customized peptide-immobilized electrode; the AuPd@GO composite acts as carrier to immobilize acetyl antibody, thus constructing a sandwich-type electrochemical immunosensor via an antigen and antibody interaction; importantly, a distinct electrochemical signal could be caught due to the AuPd@GO nanomaterial with a favorable electrocatalytic property to the commercialized 3,3,5′,5′-tetramethyl benzidine solution (TMB). Taking advantage of AuPd@GO composite, the established immunosensor displays a wide linear range from 1 pM to 1000 nM, and the detection limit is 0.5 pM (S/N = 3) for HAT p300. Next, the biosensor is also used to analyze the inhibitor of HAT p300 successfully, which is promising for promoting the development of electrochemical HAT-related biodetection and drug discovery.

Graphical abstract

A sensitive electrochemical immunoassay for testing HAT p300 based on both graphene-assisted supported AuPd nanomaterial (AuPd@GO composite) and a typical amperometric i-t technique with fast response is developed favorably.


Graphene-assisted supported AuPd nanomaterial Electrochemical immunoassay Histone acetylase Inhibitor Amperometric i-t curve 


Funding information

This work received financial support from the National Natural Science Foundation of China (21605089 and 81773483), the Ningbo Municipal Natural Science Foundation (2017A610231 and 2018A610217), the Open Subject of State Key Laboratory of Chemo/Biosensing and Chemometrics (2016001), and Zhejiang Provincial Natural Science Foundation of China (LGF18B070002). This work was also sponsored by K.C. Wong Magna Fund in Ningbo University.

Compliance with ethical standards

This study was approved by the Regional Ethics Committee of the Hunan Provincial People’s Hospital, and the blood and urine samples from the Hunan Provincial People’s Hospital in this study are permitted by patients through signed informed consents.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_2112_MOESM1_ESM.pdf (190 kb)
ESM 1 (PDF 190 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Clinical Laboratory of Hunan Provincial People’s HospitalThe First Affiliated Hospital of Hunan Normal UniversityChangshaChina
  2. 2.Blood Transfusion DepartmentXiangya Hospital of Central South UniversityChangshaChina
  3. 3.Faculty of Materials Science and Chemical EngineeringNingbo UniversityNingboChina
  4. 4.State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityChangshaChina

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