Analytical and Bioanalytical Chemistry

, Volume 411, Issue 27, pp 7127–7136 | Cite as

Construction of a novel cell-trappable fluorescent probe for hydrogen sulfide (H2S) and its bio-imaging application

  • Xiangpeng Lin
  • Yunling Chen
  • Shoujuan Wang
  • Keyin LiuEmail author
  • Fangong KongEmail author
Research Paper


Fluorescence detection of H2S in living organisms is greatly advantageous because it is nondestructive and can be used for in situ analysis. We have constructed a novel rhodamine analogue dye (Rho630) by extending the conjugated system of rhodamine to create a novel cell-trappable H2S fluorescent probe Rho630-AM-H2S with red light emission. Its application for H2S fluorescence detection in living HeLa cells and zebrafish was investigated. As expected, Rho630-AM-H2S showed a huge fluorescence turn-on response of about 20-fold at 630 nm and good selectivity toward H2S in solution. An MTT assay demonstrated that the probe showed negligible cytotoxicity in the concentrations typically used in fluorescence imaging experiments. Cell imaging experiments revealed that compared with compound 4 without cell-trappable unit modification, Rho630-AM-H2S exhibited remarkably enhanced cell penetration ability, as an enormous fluorescence signal increase was observed at the red channel within 5 min after Rho630-AM-H2S was incubated with HeLa cells. Finally, the probe Rho630-AM-H2S was used to detect H2S in living HeLa cells and zebrafish with great fluorescence enhancement in the red channel.

Graphical abstract


Rhodamine analogue Cell-trappable probe H2S detection Bio-imaging 


Funding information

This work was financially supported by NSFC (61605060, 31600472, 31570566, and 31800499), the Natural Science Foundation of Shandong Province (ZR2017LEM009), the Foundation of Key Laboratory of Pulp and Paper Science and Technology of Ministry of Education/Shandong Province of China (Nos. ZR201707 and ZR201710), the Key Research and Development Program of Shandong Province (No. 2019GSF107052; 2017GSF17130), the Foundation of Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control of China (KF201717), and the Undergraduate Innovation and Entrepreneurship Program.

Compliance with ethical standards

All animal procedures for this study were approved by the Animal Ethical Experimentation Committee of Shandong University according to the requirements of the National Act on the use of experimental animals (China).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2090_MOESM1_ESM.pdf (1.1 mb)
ESM 1 (PDF 1164 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp & Paper Science and Technology of Shandong Province/Ministry of Education, Qilu University of TechnologyShandong Academy of SciencesJinanChina

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