Advertisement

Analytical and Bioanalytical Chemistry

, Volume 411, Issue 27, pp 7055–7059 | Cite as

Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools

  • Natalia A. BurmistrovaEmail author
  • Pavel S. Pidenko
  • Sergei A. Pidenko
  • Yulia S. Skibina
  • Yulia B. Monakhova
Communication

Abstract

A new perspective on the relevant problem—creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format—was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 μg/mL, respectively. Partial least squares regression (PLS) was utilized for modeling of LPG HCMOF spectral data and quantitative analysis of BSA, OVA, total protein, and IgG in binary and ternary mixtures. Rather high coefficients of determination (R2) and low root mean square error for the calibration (RMSEC) (15%) and prediction (RMSEP) (20%) were obtained for all PLS models. The proposed approach was tested in the analysis of BSA in spiked horse blood hemolyzed (HBH). The results demonstrated the functionality of the proposed approach and offered the opportunity for the creation of a wide range of sensors for protein determination in complex mixtures.

Graphical abstract

Keywords

Microstructured optical fibers Long-period grating fiber Protein determination Chemometrics Partial least squares regression 

Notes

Funding information

The reported study was funded by the Russian Foundation for Basic Research according to the research project № 18-29-08033 and Russian Ministry of Science and Education, project 4.1063.2017/4.6.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2085_MOESM1_ESM.pdf (690 kb)
ESM 1 (PDF 690 kb)

References

  1. 1.
    Chang SKC, Zhang Y. Protein analysis. Cham: Springer; 2017.CrossRefGoogle Scholar
  2. 2.
    Huang Q, Yang L, Luo J, Guo L, Wang Z, Yang X, et al. SWATH enables precise label-free quantification on proteome scale. Proteomics. 2015;15:1215–23.  https://doi.org/10.1002/pmic.201400270.CrossRefPubMedGoogle Scholar
  3. 3.
    Crutchfield CA, Thomas SN, Sokoll LJ, Chan DW. Advances in mass spectrometry-based clinical biomarker discovery. Clin Proteomics. 2016;13:1.  https://doi.org/10.1186/s12014-015-9102-9.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Kussrow A, Enders CS, Bornhop DJ. Interferometric methods for label-free molecular interaction studies. Anal Chem. 2012;84:779–92.  https://doi.org/10.1021/ac202812h.CrossRefPubMedGoogle Scholar
  5. 5.
    Villatoro J, Zubia J. New perspectives in photonic crystal fibre sensors. Opt Laser Technol. 2016;78:67–75.  https://doi.org/10.1016/J.OPTLASTEC.2015.07.025.CrossRefGoogle Scholar
  6. 6.
    Currivan S, Upadhyay N, Paull B. Multi-channel capillaries and photonic crystal fibres for separation sciences. TrAC Trends Anal Chem. 2018;102:322–31.  https://doi.org/10.1016/J.TRAC.2018.03.008.CrossRefGoogle Scholar
  7. 7.
    Skibina YS, Tuchin VV, Beloglazov VI, Steinmeyer G, Betge JL, Wedell R, et al. Photonic crystal fibres in biomedical investigations. Quantum Electron. 2011;41:284–301.  https://doi.org/10.1070/QE2011v041n04ABEH014536.CrossRefGoogle Scholar
  8. 8.
    Buswell SC, Wright VA, Buriak JM, Van V, Evoy S. Specific detection of proteins using photonic crystal waveguides. Opt Express. 2008;16:15949.  https://doi.org/10.1364/OE.16.015949.CrossRefPubMedGoogle Scholar
  9. 9.
    Islam M, Ali M, Lai M-H, Lim K-S, Ahmad H. Chronology of Fabry-Perot interferometer fiber-optic sensors and their applications: a review. Sensors. 2014;14:7451–88.  https://doi.org/10.3390/s140407451.CrossRefPubMedGoogle Scholar
  10. 10.
    Pidenko SA, Burmistrova NA, Shuvalov AA, Chibrova AA, Skibina YS, Goryacheva IY. Microstructured optical fiber-based luminescent biosensing: is there any light at the end of the tunnel?—a review. Anal Chim Acta. 2018;1019:14–24.  https://doi.org/10.1016/J.ACA.2017.12.010.CrossRefPubMedGoogle Scholar
  11. 11.
    Pomerantsev AL. Chemometrics in Excel. Wiley; 2014.Google Scholar
  12. 12.
    Simpson RJ, Adams PD, Peter D, Golemis E. Basic methods in protein purification and analysis: a laboratory manual. Cold Spring Harbor Laboratory Press; 2009.Google Scholar
  13. 13.
    Brereton RG, Jansen J, Lopes J, Marini F, Pomerantsev A, Rodionova O, et al. Chemometrics in analytical chemistry—part II: modeling, validation, and applications. Anal Bioanal Chem. 2018;410:6691–704.  https://doi.org/10.1007/s00216-018-1283-4.CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Natalia A. Burmistrova
    • 1
    Email author
  • Pavel S. Pidenko
    • 1
  • Sergei A. Pidenko
    • 1
  • Yulia S. Skibina
    • 2
  • Yulia B. Monakhova
    • 1
    • 3
  1. 1.Institute of ChemistrySaratov State UniversitySaratovRussia
  2. 2.SPE LLC Nanostructured Glass TechnologySaratovRussia
  3. 3.Spectral Service AGCologneGermany

Personalised recommendations