Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level

  • Yue Wang
  • Yadi Wang
  • Lina Huang
  • Xiangyong LiuEmail author
  • Jun Hu
  • Junhong LüEmail author
Research Paper


Endothelial damage is a major manifestation in many forms of heart and lung injuries induced by lipopolysaccharide (LPS), but the biochemical responses and activation mechanisms of endothelial cells have not been fully explicit. In this study, the biochemical changes to endothelial cells exposed to LPS were investigated by synchrotron FTIR microspectroscopy at a single-cell level. We found that the whole infrared spectrum of endothelial cells shifted after LPS treatment, indicating chemical component changes within cells. Principal component analysis (PCA) and t tests on subspectra (fatty acid region, protein region, and nucleic acid-sugar region, respectively) further showed that sugar components as well as fatty acids changed dramatically while proteins had no significant variation following LPS exposure. These results suggested that the glycocalyx layer structure on endothelial cell membrane may be mainly influenced by LPS and also proved that synchrotron FTIR microspectroscopy was a useful technique to evaluate the biochemical changes of endothelial damage at the single-cell level.

Graphical abstract


Endothelial cells Lipopolysaccharide Synchrotron FTIR microspectroscopy Single-cell analysis Glycocalyx 



We thank the staff of BL01B beamline at the National Center for Protein Sciences Shanghai and Shanghai Synchrotron Radiation Facility for assistance during data collection.

Funding information

This work was supported by the National Natural Science Foundation of China (No U1732130), Key Research Program of Frontier Sciences of the Chinese Academy of Sciences (QYZDJ-SSW-SLH019), and the Scientific Research Foundation of Binzhou Medical University (BY2017KYQD02).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of PharmacyBinzhou Medical UniversityYantaiChina
  2. 2.Zhangjiang Lab, Shanghai Advanced Research InstituteChinese Academy of SciencesShanghaiChina
  3. 3.CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghaiChina
  4. 4.University of Chinese Academy of SciencesBeijingChina

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