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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 26, pp 6801–6811 | Cite as

Development and comparison of mimotope-based immunoassays for the analysis of fumonisin B1

  • Riikka Peltomaa
  • Irene Agudo-Maestro
  • Vicente Más
  • Rodrigo Barderas
  • Elena Benito-PeñaEmail author
  • María C. Moreno-BondiEmail author
Paper in Forefront

Abstract

Mycotoxins can be found as natural contaminants in many foods and feeds, and owing to their toxic effects, it is essential to detect them before they enter the food chain. An interesting approach for the analysis of mycotoxins by competitive immunoassays is the use of epitope-mimicking peptides, or mimotopes, which can replace the toxin conjugates traditionally used in such assays. Mimotopes can be selected from phage-displayed peptide libraries even without any prior knowledge of the antibody–antigen interaction, and after identifying the target specific clones, individual clones can be efficiently amplified in bacteria and used directly in the immunoassay. Following such approach, we have previously selected and identified a dodecapeptide which functions as a mimotope for the mycotoxin fumonisin B1. In this work, we present the development and comparison of various immunoassays based on this mimotope, named A2, which has been used in the phage-displayed format in which it was selected, but also as a fluorescent recombinant fusion protein or as a synthetic peptide. The highest sensitivity was obtained with a magnetic bead–based assay using the synthetic peptide and enzymatic detection which provided a detection limit of 0.029 ng mL−1. Analysis of the binding kinetics by surface plasmon resonance (SPR) further reinforced the suitability of the synthetic peptide for the competitive immunoassays, as this mimotope showed a slightly lower affinity for the target antibody in comparison with the recombinant fusion protein.

Graphical abstract

Keywords

Mycotoxin Immunoassay Mimotope Peptide Phage Magnetic bead 

Notes

Acknowledgments

R.P. acknowledges UCM for a pre-doctoral grant and R.B. the PI17CIII/00045 grant from the AES-ISCIII program.

Funding information

This research was funded by the Spanish Ministry of Science, Innovation and Universities, grant RTI2018-096410-B-C21.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of ChemistryComplutense UniversityMadridSpain
  2. 2.Centro Nacional de MicrobiologíaInstituto de Salud Carlos IIIMadridSpain
  3. 3.Chronic Disease Programme (UFIEC)Instituto de Salud Carlos IIIMadridSpain

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