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Photoelectrochemical detection of breast cancer biomarker based on hexagonal carbon nitride tubes

  • Junjun Luo
  • Dong Liang
  • Xiaoqing Qiu
  • Minghui YangEmail author
Research Paper

Abstract

Photoelectrochemical (PEC) sensor for sensitive detection of breast cancer biomarker human epidermal growth factor receptor 2 (HER2) utilizing hexagonal carbon nitride tubes (HCNT) as photoactive material is reported. The detection is based on suppression of the PEC current intensity of the sensor. HCNT were synthesized via a facile hydrothermal method with large specific surface area and low electron-hole recombination. Au nanoparticles (AuNPs) were deposited onto the surface of the HCNT, which enhanced the photocurrent intensity of the HCNT by one time. For HER2 detection, peptide specific to HER2 was immobilized on the AuNPs surface for capturing HER2 molecules. The following binding of HER2 with HER2 aptamer and the reaction of phosphate groups on aptamer with molybdate can form molybdophosphate precipitate, which sticks to the surface of HCNT and impedes electron transport. Thus, photocurrent intensity of the sensor was suppressed. Under optimal conditions, the linear relationship between the PEC intensity and the logarithm of HER2 concentration was from 0.5 to 1 ng mL−1 with low limit of detection (LOD) of 0.08 pg mL−1. Furthermore, the PEC sensor also displayed capability for detecting HER2 in human serum samples. This PEC sensor signal detection strategy can be easily adapted to other PEC sensors involving DNA and find wide applications.

Graphical abstract

Keywords

Photoelectrochemistry Breast cancer Hexagonal carbon nitride tube Au nanoparticle Molybdophosphate 

Notes

Funding information

This work received support from the National Natural Science Foundation of China (No. 21575165).

Compliance with ethical standards

The authors declare that they have no competing interests. All experiments were in accordance with the guidelines of the National Institute of Health, China, and approved by the Institutional Ethical Committee (IEC) of the Second Xiangya Hospital that attached to Central South University. We also obtained informed consent for any experimentation with human serum samples.

Supplementary material

216_2019_2060_MOESM1_ESM.pdf (226 kb)
ESM 1 (PDF 225 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Junjun Luo
    • 1
  • Dong Liang
    • 1
  • Xiaoqing Qiu
    • 1
  • Minghui Yang
    • 1
    Email author
  1. 1.Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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