Advertisement

An efficient turn-on fluorescence biosensor for the detection of glutathione based on FRET between N,S dual-doped carbon dots and gold nanoparticles

  • Wenjuan DongEmail author
  • Ruiping Wang
  • Xiaojuan GongEmail author
  • Chuan Dong
Research Paper
  • 28 Downloads

Abstract

Fluorescence resonance energy transfer (FRET) is a kind of energy transfer mechanism depending on the distance between donor and acceptor, which exhibited potential application in biosensors. In this study, an efficient fluorescence “turn-on” strategy for the detection of glutathione (GSH) has been established based on FRET between nitrogen and sulfur dual-doped carbon dots (N,S-CDs) and gold nanoparticles (Au NPs). A novel N,S-CDs was synthesized by a one-pot hydrothermal treatment of 3-aminothiophenol, which possessed excellent fluorescence property with the maximum emission wavelength of 530 nm. Then, the as-prepared N,S-CDs served as energy donor to transfer energy to Au NPs via FRET process, resulting in fluorescence quenching of N,S-CDs. However, the fluorescence of N,S-CDs was recovered efficiently by adding GSH into the mixture solution of N,S-CDs and Au NPs. Therefore, the FRET assembly of N,S-CDs and Au NPs was used as a fluorescence probe for the “turn-on” sensing GSH with the linear range from 3.8 to 415.1 μM and the limit detection of 0.21 μM. This nanosensor platform was employed to monitor GSH in serum samples with satisfying results.

Graphical abstract

Keywords

N,S dual-doped carbon dots Gold nanoparticles Fluorescence resonance energy transfer Glutathione 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21705101), Natural Science Foundation of Shanxi Province (No. 201801D121040), and China Postdoctoral Science Foundation (No. 2018M642969).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval and consent to participate

This study conformed to the ethical guidelines of the Declaration of Helsinki and was approved by the Shanxi University Institutional Review Board Office. All human serum samples are supplied by the healthy volunteers with their informed consent.

Supplementary material

216_2019_2042_MOESM1_ESM.pdf (241 kb)
ESM 1 (PDF 240 kb)

References

  1. 1.
    Singh VK, Yadav PK, et al. Peroxidase mimetic activity of fluorescent N,S-carbon quantum dots and their application in colorimetric detection of H2O2 and glutathione in human blood serum. J Mater Chem B. 2018;6:5256–68.CrossRefGoogle Scholar
  2. 2.
    Gao Y, Wu KL, Li HY, Chen W, Fu M, Yue K, et al. Glutathione detection based on peroxidase-like activity of Co3O4-montmorillonite nanocomposites. Sensors Actuators B Chem. 2018;273:1635–9.CrossRefGoogle Scholar
  3. 3.
    Wu R, Ge HG, Liu CF, Zhang SH, Hao L, Zhang Q, et al. A novel thermometer-type hydrogel senor for glutathione detection. Talanta. 2019;196:191–6.CrossRefGoogle Scholar
  4. 4.
    Peng HP, Jian ML, Huang ZN, Wang WJ, Deng HH, Wu WH, et al. Facile electrochemiluminescence sensing platform based on high-quantum yield gold nanocluster probe for ultrasensitive glutathione detection. Biosens Bioelectron. 2018;105:71–6.CrossRefGoogle Scholar
  5. 5.
    Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid. J Pharm Biomed. 2008;48:1375–80.CrossRefGoogle Scholar
  6. 6.
    Saha A, Jana NR. Detection of cellular glutathione and oxidized glutathione using magnetic-plasmonic nanocomposite based turn-off surface enhanced Raman scattering. Anal Chem. 2013;85:9221–8.CrossRefGoogle Scholar
  7. 7.
    Chen LY, Jong SP, Wu D, Cheol HK, Yoon JY. A colorimetric and fluorescent probe for rapid detection of glutathione and its application to tissue specific bioimaging in living cells and zebrafish. Sensors Actuators B Chem. 2018;262:306–12.CrossRefGoogle Scholar
  8. 8.
    Burford N, Eelman MD, Mahony DE, Morash M. Definitive identification of cysteine and glutathione complexes of bismuth by mass spectrometry: assessing the biochemical fate of bismuth pharmaceutical agents. Chem Commun. 2003;14:146–7.CrossRefGoogle Scholar
  9. 9.
    Hodáková J, Preisler J, Foret F, Kubán P. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. J Chromatogr A. 2015;1391:102–8.CrossRefGoogle Scholar
  10. 10.
    Wawegama NK, Browning GF, Kanci A, Marenda MS, Markham PF. Development of a recombinant protein-based enzyme-linked immunosorbent assay for diagnosis of Mycoplasma bovis infection in cattle. Clin Vaccine Immunol. 2014;21:196–202.CrossRefGoogle Scholar
  11. 11.
    Chen W, Wu XD, Pu L. Highly selective fluorescent recognition of glutathione by using a water soluble binaphthyl aldehyde. Tetrahedron Lett. 2017;58:1781–3.CrossRefGoogle Scholar
  12. 12.
    Qin J, Zhang LM, Yang R. Powder carbonization to synthesize novel carbon dots derived from uricacid for the detection of Ag(I) and glutathione. Spectrochim Acta A Mol Biomol Spectrosc. 2019;207:54–60.CrossRefGoogle Scholar
  13. 13.
    Niu LY, Guan YS, Chen YZ, Tung CH, Yang QZ. BODIPY-based ratiometric fluorescent sensor for highly selective detection of glutathione over cysteine and homocysteine. J Am Chem Soc. 2012;134:18928–31.CrossRefGoogle Scholar
  14. 14.
    Shao N, Jin J, Wang H, Zheng J, Yang R, Chan W, et al. Design of bis-spiropyran ligands as dipolar molecule receptors and application to in vivo glutathione fluorescent probes. J Am Chem Soc. 2010;132:725–36.CrossRefGoogle Scholar
  15. 15.
    Yang CL, Deng WP, Liu HY, Ge SG, Yan M. Turn-on fluorescence sensor for glutathione in aqueous solutions using carbon dots–MnO2 nanocomposites. Sensors Actuators B Chem. 2015;216:286–92.CrossRefGoogle Scholar
  16. 16.
    Cai QY, Li J, Ge J, Zhang L, Hu YL, Li ZH, et al. A rapid fluorescence “switch-on” assay for glutathione detection by using carbon dots-MnO2 nanocomposites. Biosens Bioelectron. 2015;72:31–6.CrossRefGoogle Scholar
  17. 17.
    Xiang HJ, Tham HP, Nguyen MD, Phua SZF, Lim WQ, Liu JG, et al. An aza-BODIPY based near-infrared fluorescent probe for sensitive discirmination of cysteine/homocysteine and glutathione in living cells. Chem Commun. 2017;53:5220–3.CrossRefGoogle Scholar
  18. 18.
    Deng JH, Lu QJ, Hou YX, Liu ML, Li HT, Zhang YY, et al. Nanosensor composed of nitrogen-doped carbon dots and gold nanoparticles for highly selective detection of cysteine with multiple signals. Anal Chem. 2015;87:2195–203.CrossRefGoogle Scholar
  19. 19.
    Zhang Q, Gong Y, Guo XJ, Zhang P, Ding CF. Multifunctional gold nanoparticle-based fluorescence resonance energy-transfer probe for target drug delivery and cell fluorescence imaging. ACS Appl Mater Interfaces. 2018;10:34840–8.CrossRefGoogle Scholar
  20. 20.
    Han X, Han M, Ma L, Qu F, Kong RM, Qu FL. Self-assembled gold nanoclusters for fluorescence turn-on and colorimetric dual-readout detection of alkaline phosphatase activity via DCIP-mediated fluorescence resonance energy transfer. Talanta. 2019;194:55–62.CrossRefGoogle Scholar
  21. 21.
    Wang J, Song FJ, Ai YL, Hu SW, Huang ZZ, Zhong WY. A simple FRET system using two-color CdTe quantum dots assisted by cetyltrimethylammonium bromide and itsapplication to Hg(II) detection. Lumin. 2019;34:205–11.CrossRefGoogle Scholar
  22. 22.
    Chen GW, Song FL, Xiong XQ, Peng XJ. Fluorescent nanosensors based on fluorescence resonance energy transfer (FRET). Ind Eng Chem Res. 2013;52:11228–45.CrossRefGoogle Scholar
  23. 23.
    Jing N, Tian M, Wang YT, Zhang Y. Nitrogen-doped carbon dots synthesized from acrylic acid andethylenediamine for simple and selective determination of cobalt ions in aqueous media. J Lumin. 2019;206:169–75.CrossRefGoogle Scholar
  24. 24.
    Gong XJ, Li ZB, Hu Q, Zhou RX, Shuang SM, Dong C. N,S,P Co-doped carbon nanodot fabricated from waste microorganism and its application for label-free recognition of manganese(VII) and L-ascorbic acid and and logic gate operation. ACS Appl Mater Interfaces. 2017;9:38761–72.CrossRefGoogle Scholar
  25. 25.
    Ding H, Zhou XX, Qin BT, Zhou ZY, Zhao YP. Highly fluorescent near-infrared emitting carbon dots derived from lemonjuice and its bioimaging application. J Lumin. 2019;211:298–304.CrossRefGoogle Scholar
  26. 26.
    Ahmed SR, Kim J, Suzuki T, Lee J, Park EY. Enhanced caalytic activity of gold nanoparticle-carbon nanotube hybrids for influenza virus detection. Biosens Bioelectron. 2016;85:503–8.CrossRefGoogle Scholar
  27. 27.
    Yang YX, Huo DQ, Wu HX, Wang XF. N, P-doped carbon quantum dots as a fluorescent sensing platform for carbendazim detection based on fluorescence resonance energy transfer. Sensors Actuators B Chem. 2018;274:296–303.CrossRefGoogle Scholar
  28. 28.
    Wu XL, Song Y, Yan X, Zhu CZ, Ma YQ, Du D, et al. Carbon quantum dots as fluorescence resonance energy transfer sensors for organophosphate pesticides determination. Biosens Bioelectron. 2017;94:292–7.CrossRefGoogle Scholar
  29. 29.
    Niu WJ, Shan D, Zhu RH, Deng SY, Ceng S, Zhang XJ. Dumbbell-shaped carbon quantum dots/AuNCs nanohybrid as an efficient ratiometric fluorescent probe for sensing cadmium (II) ionsand L-ascorbic acid. Carbon. 2016;96:1034–42.CrossRefGoogle Scholar
  30. 30.
    Meng FY, Chai H, Ma XY, Tang YG, Miao P. FRET investigation toward DNA tetrahedron-based ratiometric analysis of intracellular telomeraseactivity. J Mater Chem B. 2019;7:1926–32.CrossRefGoogle Scholar
  31. 31.
    Shi J, Chan C, Pang Y, Ye W, Tian F, Lyu J, et al. A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcusaureus. Biosens Bioelectron. 2015;67:595–600.CrossRefGoogle Scholar
  32. 32.
    Zhao WA, Brook MA, Li YF. Design of gold nanoparticle-based colorimetric biosensing assays. Chembiochem. 2008;9:2363–71.CrossRefGoogle Scholar
  33. 33.
    Gong XJ, Wang HP, Liu Y, Hu Q, Gao YF, Yang ZH, et al. A di-functional and label-free carbon-based chem-nanosensor for real-time monitoring of pH fluctuation and quantitative determining of curcumin. Anal Chim Acta. 2019;1057:132–44.Google Scholar
  34. 34.
    Fan RJ, Sun Q, Zhang L, Zhang Y, Lu AH. Photoluminescent carbon dodirectly derived from polyethylene glycol and their application for cellular imaging. Carbon. 2014;71:87–93.CrossRefGoogle Scholar
  35. 35.
    Kalytchuk S, Polakova K, Wang Y, Froning JP, Cepe K, Rogach AL, et al. Carbon dot nanothermometry: intracellular photoluminescence lifetime thermal sensing. ACS Nano. 2017;11:1432–42.CrossRefGoogle Scholar
  36. 36.
    Jovin TM, Wieb van der Meer B, Hildebrandt N, Sapsford KE, Pons T, Campbell RE, et al. Outlook on FRET: the future of resonance energy transfer. FRET—Förster resonance energy transfer. Wiley-VCH; 2013. p. 757–65.Google Scholar
  37. 37.
    Yu FB, Li P, Wang BS, Han KL. Reversible near-infrared fluorescent probe introducing tellurium to mimetic glutathione peroxidase for monitoring the redoxcycles between peroxynitrite and glutathione in vivo. J Am Chem Soc. 2013;135:7674–80.CrossRefGoogle Scholar
  38. 38.
    Pan JH, Zheng ZY, Yang JY, Wu YY, Lu FS, Chen YW, et al. A novel and sensitive fluorescence sensor for glutathione detection by controlling the surface passivation degree of carbon quantum dots. Talanta. 2017;166:1–7.CrossRefGoogle Scholar
  39. 39.
    Shi Y, Pen Y, Zhang H, Zhang Z, Li MJ, Yi C, et al. A dual-mode nanosensor based on carbon quantum dots and gold nanoparticles for discriminative detection of glutathionein human plasma. Biosens Bioelectron. 2014;56:39–45.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Environmental Science, and School of Chemistry and Chemical EngineeringShanxi UniversityTaiyuanChina

Personalised recommendations