Analytical and Bioanalytical Chemistry

, Volume 411, Issue 25, pp 6615–6624 | Cite as

Magnetic immunochromatographic test for histamine detection in wine

  • Amanda Moyano
  • María Salvador
  • José C. Martínez-García
  • Vlad Socoliuc
  • Ladislau Vékás
  • Davide Peddis
  • Miguel A. Alvarez
  • María Fernández
  • Montserrat RivasEmail author
  • M. Carmen Blanco-LópezEmail author
Research Paper
Part of the following topical collections:
  1. Nanoparticles for Bioanalysis


Histamine, a biogenic amine, is abundant in fermented foods and beverages, notably wine. A high intake of this monoamine may produce adverse reactions in humans, which may be severe in individuals with a reduced capacity to catabolise extrinsic histamine. Thus, control of histamine concentration during wine production and before distribution is advisable. Simple, rapid, point-of-use bioanalytical platforms are needed because traditional methods for the detection and quantification of histamine are expensive and time-consuming. This work applies the lateral flow immunoassay technique to histamine detection. Superparamagnetic particle labels, and an inductive sensor designed to read the test line in the immunoassay, enable magnetic quantification of the molecule. The system is calibrated with histamine standards in the interval of interest for wine production. A commercial optical strip reader is used for comparison measurements. The lateral flow system has a limit of detection of 1.2 and 1.5 mg/L for the inductive and optical readers, respectively. The capability of the inductive system for histamine quantification is demonstrated for wine samples at different processing points (at the end of alcoholic fermentation, at the end of malolactic fermentation, in freshly bottled wine, and in reserve wine). The results are validated by ultra-high-performance liquid chromatography.

Graphical abstract


Biogenic amines Histamine Lateral flow immunoassay Superparamagnetic nanoparticles Histamine biosensor 



Wine samples were kindly provided by Juan M. Redondo from DOP Vino de Cangas. The authors acknowledge the technical assistance of Begoña Redruello (IPLA) in the chromatographic analysis in wine samples.

Funding information

This work was financially supported by the Spanish Ministry of Economy and Competitiveness under projects MAT2017-84959-C2-1-R, MAT2016-81955-REDT, and AGL2016-78708-R; the Council of Gijón-IUTA under grant SV-18-GIJON-1-27; and the Principality of Asturias under project IDI/2018/000185.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physical and Analytical ChemistryUniversity of OviedoOviedoSpain
  2. 2.Department of PhysicsUniversity of OviedoGijónSpain
  3. 3.Institute of Structure of Matter (CNR)Monterotondo ScaloItaly
  4. 4.Laboratory of Magnetic Fluids, Center for Fundamental and Advanced Technical ResearchRomanian Academy - Timisoara BranchTimisoaraRomania
  5. 5.Dairy Research Institute of Asturias, IPLA (CSIC)VillaviciosaSpain

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