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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 24, pp 6409–6417 | Cite as

Two-dimensional decomposition of H-D exchange mass spectra of multicharged ions of biopolymers and their separation into components with independent H-D substitutions

  • Valeriy Raznikov
  • Marina RaznikovaEmail author
  • Il’ya Sulimenkov
Research Paper

Abstract

The work is aimed at developing a numerical method for analysing mass spectra of deutero-substituted multicharged ions of biopolymers to determine contributions of components presumably corresponding to different biomolecule conformations. The two-dimensional decomposition of the H-D exchange mass spectra of two, three and four charged apamin ions with their separation suggests that the reaction of apamin ions with ND3 molecules in the gas phase reveals hypothetically three different structural modifications of apamin ions. Usually for H-D exchange mass spectra, the presence of many resolvable protein structures was determined from measured distributions of peak intensities of ions with the same charge state. The method is new and has no published analogues.

Graphical abstract

Keywords

Mass spectrometry H-D exchange Electrospray ionization (ESI) Multiply charged ions Decomposition of deutero-substituted charge-state distributions Retention probability for protons and deutrons 

Notes

Acknowledgments

The authors appreciate Dr. V.I. Kozlovskiy and Dr. A.V. Chudinov for their aid for getting experimental data for apamin. Our thanks to A.R. Piktelev for further development of his software for time-of-flight mass spectra data processing.

Funding information

This work was supported partially by the Russian Academy of Sciences (program no. 36). This work was performed in accordance with the state task, state registration N 0089-2019-0018.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Valeriy Raznikov
    • 1
  • Marina Raznikova
    • 2
    Email author
  • Il’ya Sulimenkov
    • 1
  1. 1.Chernogolovka Branch of the N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of SciencesChernogolovka, Moscow regionRussia
  2. 2.Institute of Problems of Chemical Physics, Russian Academy of SciencesChernogolovka, Moscow regionRussia

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