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Accurate mass and retention time library of serum lipids for type 1 diabetes research

  • Ngoc Vu
  • Monica Narvaez-Rivas
  • Guan-Yuan Chen
  • Marian J. Rewers
  • Qibin ZhangEmail author
Paper in Forefront

Abstract

Dysregulated lipid species are linked to various disease pathologies and implicated as potential biomarkers for type 1 diabetes (T1D). However, it is challenging to comprehensively profile the blood specimen lipidome with full structural details of every lipid molecule. The commonly used reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS)-based lipidomics approach is powerful for the separation of individual lipid species, but lipids belonging to different classes may still co-elute and result in ion suppression and misidentification of lipids. Using offline mixed-mode and RPLC-based two-dimensional separations coupled with MS/MS, a comprehensive lipidomic profiling was performed on human sera pooled from healthy and T1D subjects. The elution order of lipid molecular species on RPLC showed good correlations to the total number of carbons in fatty acyl chains and total number of double bonds. This observation together with fatty acyl methyl ester analysis was used to enhance the confidence of identified lipid species. The final T1D serum lipid library database contains 753 lipid molecular species with accurate mass and RPLC retention time uniquely annotated for each of the species. This comprehensive human serum lipid library can serve as a database for high-throughput RPLC-MS-based lipidomic analysis of blood samples related to T1D and other childhood diseases.

Graphical abstract

Keywords

Human serum lipidome Type 1 diabetes Lipid profiling Mixed-mode LC RPLC-MS/MS Accurate mass and time tag 

Notes

Funding

The work was partially supported by National Institutes of Health (NIH) grants R21 GM104678 and R01 DK114345. Clinical sample collection was supported by NIH grant R01 DK32493.

Compliance with ethical standards

This research analyzed de-identified human serum samples collected from clinical studies and has IRB approval as stated in the “Materials and methods” section. All authors have read and agreed to the final version of this manuscript.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_1997_MOESM1_ESM.pdf (188 kb)
ESM 1 (PDF 188 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry & BiochemistryUniversity of North Carolina at GreensboroGreensboroUSA
  2. 2.Center for Translational Biomedical ResearchUniversity of North Carolina at GreensboroKannapolisUSA
  3. 3.Barbara Davis Center for DiabetesUniversity of Colorado School of MedicineAuroraUSA

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