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Rapid detection of trace Salmonella in milk and chicken by immunomagnetic separation in combination with a chemiluminescence microparticle immunoassay

  • Jingwen Li
  • Qingjun Liu
  • Yuping Wan
  • Xiaosheng Wu
  • Yin Yang
  • Ruixue Zhao
  • Erning Chen
  • Xiaoyan Cheng
  • Meihong DuEmail author
Research Paper
  • 35 Downloads

Abstract

Rapid detection of trace Salmonella is urgently needed to ensure food safety. We present an innovative pretreatment strategy, based on a two-step enrichment culture and immunomagnetic separation, combined with a chemiluminescence microparticle immunoassay to detect at least one proliferative Salmonella cell in 25 mL (25 g) food. The capture performance of immunomagnetic beads (IMBs) of sizes for Salmonella was investigated, and the IMBs of size 2.8 μm showed a high capture efficiency of 60.7% in 25 mL milk and 74.5% in 25 mL chicken culture filtrate, which ensured the successful capture of trace Salmonella after 2.5 h in situ enrichment even from only one Salmonella cell. The separated Salmonella cells, reaching an amount of 103 colony-forming units (CFU) by a secondary enrichment for 3 h, were detected by a horseradish peroxidase chemiluminescence reaction with 4-(1-imidazolyl)phenol as an enhancer, which evidenced a linear response for Salmonella concentrations ranging from 2.3 × 102 to 7.8 × 104 CFU/mL. The entire detection process was completed within 8 h, with a very low detection limit of 1 CFU/25 mL (25 g), which was verified by colony counting, and a small degree of interference of 0.17–1.06%. Trace Salmonella from five different serovars in milk and chicken was successfully detected without false negative or false positive results. Furthermore, this study provides a basis to develop a fully automated instrument based on IMBs that includes all steps from sample preparation to chemiluminescence microparticle immunoassay for high-throughput screening of foodborne pathogens.

Graphical abstract

Keywords

Pretreatment Pathogen Chemiluminescence immunoassay Enrichment Food safety 

Notes

Acknowledgements

This work was supported by the National Key Scientific Instrument and Equipment Development Projects (grant number 2013YQ140371) and the Building of Innovative Team Plan (grant number IG201807C1).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_1991_MOESM1_ESM.pdf (172 kb)
ESM 1 (PDF 172 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jingwen Li
    • 1
  • Qingjun Liu
    • 1
  • Yuping Wan
    • 2
  • Xiaosheng Wu
    • 2
  • Yin Yang
    • 1
  • Ruixue Zhao
    • 1
  • Erning Chen
    • 1
  • Xiaoyan Cheng
    • 1
  • Meihong Du
    • 1
    Email author
  1. 1.Beijing Engineering Research Center of Food Safety Analysis, Beijing Engineering Technology Research Centre of Gene Sequencing and Gene Function Analysis, Beijing Center for Physical and Chemical AnalysisBeijingChina
  2. 2.Beijing Engineering Research Center of Food Safety Immune Rapid DetectionBeijing Kwinbon Biotechnology Co. Ltd.BeijingChina

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