Evaluation of an ionic liquid chiral selector based on clindamycin phosphate in capillary electrophoresis

  • Xiaofei Ma
  • Yingxiang DuEmail author
  • Xinqi Zhu
  • Zijie Feng
  • Cheng Chen
  • Jiangxia Yang
Research Paper


Recently, increasing attention has been given to the research on chiral ionic liquids (CILs) in chiral separation field; however, only a few literatures focus on the exploration of CILs as the sole chiral selector. In this study, an ionic liquid chiral selector based on antibiotic, namely tetramethylammonium clindamycin phosphate (TMA-CP), was originally synthesized and subsequently utilized for enantioseparation in capillary electrophoresis (CE). Remarkably improved separations of eight racemic analytes were achieved in TMA-CP system in contrast to the clindamycin phosphate (CP) system. The optimal separation conditions were determinated by systematic experiments on several crucial parameters including the type and proportion of organic modifier, CIL concentration, buffer pH, and applied voltage. Additionally, molecular modeling with AutoDock was applied to probe into the chiral recognition mechanism of the ionic liquid chiral selectors, which well corresponded with the experimental results. It is the first time that antibiotic-based ionic liquid was exploited as favorable sole chiral selector in CE, and this strategy has paved a new way for development of novel ionic liquids chiral selectors based on antibiotics.

Graphical abstract


Ionic liquids Clindamycin phosphate Capillary electrophoresis Enantioseparation Molecular docking 


Funding information

This work was supported by the Project of National Natural Science Foundation of China (No.: 81373378) and the Natural Science Foundation of Jiangsu Province (Program No.: BK20150697).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1967_MOESM1_ESM.pdf (771 kb)
ESM 1 (PDF 770 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education)China Pharmaceutical UniversityNanjingChina
  2. 2.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingChina

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