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Upconversion photoluminescence analysis of fluoroquinolones

  • Qiuju Zhou
  • Xiaoyan Deng
  • Yajun Fang
  • Kejun TanEmail author
Research Paper
  • 12 Downloads

Abstract

The characteristics of the upconversion (UC) photoluminescence (PL) of fluoroquinolones (FQs) are reported here for the first time. Using UC PL, a simple, sensitive, and cost-effective method of determining FQs was developed that eliminated interference arising from biological background fluorescence and did not require UV light to excite the FQ (in contrast to downconversion PL), which is an advantage given that UV is potentially damaging to organisms. Ciprofloxacin (CPFX) and levofloxacin (LVFX), two important FQs, were studied. The effects of acidity, temperature, the solvent used, ionic strength, and stable time on the UC PL from the two FQs were also investigated. The UC PL intensity was found to be proportional to the concentration of CPFX over the range 0.05–100 μmol/L with a correlation coefficient of 0.9992, and proportional to the concentration of LVFX over the range 0.05–100 μmol/L with a correlation coefficient of 0.9991. The limit of detection (LOD) was 6.05 nmol/L for CPFX and 5.64 nmol/L for LVFX. The proposed method was successfully used to determine FQs in human serum and pharmaceutical samples. The recoveries of the two FQs ranged from 96.0% to 103.2% and the RSD was < 2.62%.

Graphical abstract

Keywords

Upconversion Photoluminescence Fluoroquinolone Ciprofloxacin Levofloxacin 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21377103).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

This study was approved by The Laboratory Animal Ethics Review Committee for Southwest University and was performed in accordance with its ethical standards. Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Lu W, Liu J, Li J, et al. Dual-template molecularly imprinted polymers for dispersive solid-phase extraction of fluoroquinolones in water samples coupled with high performance liquid chromatography. Analyst. 2019;144:1292–302.Google Scholar
  2. 2.
    Seifrtova M, Novakova L, Lino C, et al. An overview of analytical methodologies for the determination of antibiotics in environmental waters. Anal Chim Acta. 2009;649(2):158–79.Google Scholar
  3. 3.
    Cao L, Kong D, Sui J, et al. Broad-specific antibodies for a generic immunoassay of quinolone: development of a molecular model for selection of haptens based on molecular field-overlapping. Anal Chem. 2009;81(9):3246–51.Google Scholar
  4. 4.
    Gao M, Wang J, Song X, et al. An effervescence-assisted switchable fatty acid-based microextraction with solidification of floating organic droplet for determination of fluoroquinolones and tetracyclines in seawater, sediment, and seafood. Anal Bioanal Chem. 2018;410(11):2671–87.Google Scholar
  5. 5.
    Dewitte B, Dewulf J, Demeestere K, et al. Ozonation of ciprofloxacin in water: HRMS identification of reaction products and pathways. Environ Sci Technol. 2008;42(13):4889–95.Google Scholar
  6. 6.
    Hu G, Sheng W, Zhang Y, et al. A novel and sensitive fluorescence immunoassay for the detection of fluoroquinolones in animal-derived foods using upconversion nanoparticles as labels. Anal Bioanal Chem. 2015;407(28):8487–96.Google Scholar
  7. 7.
    Wammer KH, Korte AR, Lundeen RA, et al. Direct photochemistry of three fluoroquinolone antibacterials: norfloxacin, ofloxacin, and enrofloxacin. Water Res. 2013;47(1):439–48.Google Scholar
  8. 8.
    Vakh C, Pochivalov A, Koronkiewicz S, et al. A chemiluminescence method for screening of fluoroquinolones in milk samples based on a multi-pumping flow system. Food Chem. 2019;270:10–6.Google Scholar
  9. 9.
    Codex Alimentarius Commission. Joint FAO/WHO Food Standards Programme. Rome: Codex Alimentarius Commission; 2012.Google Scholar
  10. 10.
    Ministry of Agriculture. Gazette no. 2292. Beijing: Ministry of Agriculture of the People’s Republic of China; 2015.Google Scholar
  11. 11.
    Alcaráz MR, Vera-Candioti L, Culzoni MJ, et al. Ultrafast quantitation of six quinolones in water samples by second-order capillary electrophoresis data modeling with multivariate curve resolution-alternating least squares. Anal Bioanal Chem. 2014;406(11):2571–80.Google Scholar
  12. 12.
    Attia MS, Essawy AA, Youssef AO. Europium-sensitized and simultaneous pH-assisted spectrofluorimetric assessment of ciprofloxacin, norfloxacin and gatifloxacin in pharmaceutical and serum samples. J Photochem Photobiol A. 2012;236:26–34.Google Scholar
  13. 13.
    Qu S, Wang X, Tong C, et al. Metal ion mediated molecularly imprinted polymer for selective capturing antibiotics containing beta-diketone structure. J Chromatogr A. 2010;1217(52):8205–11.Google Scholar
  14. 14.
    Xiao D, Dramou P, Xiong N, et al. Preparation of molecularly imprinted polymers on the surface of magnetic carbon nanotubes with a pseudo template for rapid simultaneous extraction of four fluoroquinolones in egg samples. Analyst. 2013;138(11):3287–96.Google Scholar
  15. 15.
    Schulte S, Ackermann T, Bertram N, et al. Determination of the newer quinolones levofloxacin and moxifloxacin in plasma by high-performance liquid chromatography with fluorescence detection. J Chromatogr Sci. 2006;44(4):205–8.Google Scholar
  16. 16.
    Idowu OR, Peggins JO. Simple, rapid determination of enrofloxacin and ciprofloxacin in bovine milk and plasma by high-performance liquid chromatography with fluorescence detection. J Pharm Biomed Anal. 2004;35(1):143–53.Google Scholar
  17. 17.
    Meng Z, Shi Z, Liang S, et al. Residues investigation of fluoroquinolones and sulphonamides and their metabolites in bovine milk by quantification and confirmation using ultra-performance liquid chromatography-tandem mass spectrometry. Food Chem. 2015;174:597–605.Google Scholar
  18. 18.
    Zhang X, Wei Y, Ding Y. Electrocatalytic oxidation and voltammetric determination of ciprofloxacin employing poly(alizarin red)/graphene composite film in the presence of ascorbic acid, uric acid and dopamine. Anal Chim Acta. 2014;835:29–36.Google Scholar
  19. 19.
    Li YT, Qu LL, Li DW, et al. Rapid and sensitive in-situ detection of polar antibiotics in water using a disposable Ag-graphene sensor based on electrophoretic preconcentration and surface-enhanced Raman spectroscopy. Biosens Bioelectron. 2013;43:94–100.Google Scholar
  20. 20.
    Chatterjee DK, Gnanasammandhan MK, Zhang Y. Small upconverting fluorescent nanoparticles for biomedical applications. Small. 2010;6(24):2781–95.Google Scholar
  21. 21.
    Haase M, Schäfer H. Upconverting nanoparticles. Angew Chem Int Edit. 2011;50(26):5808–29.Google Scholar
  22. 22.
    Zhou J, Liu Z, Li F. Upconversion nanophosphors for small-animal imaging. Chem Soc Rev. 2012;41(3):1323–49.Google Scholar
  23. 23.
    Long Q, Li H, Zhang Y, et al. Upconversion nanoparticle-based fluorescence resonance energy transfer assay for organophosphorus pesticides. Biosens Bioelectron. 2015;68:168–74.Google Scholar
  24. 24.
    Duan N, Wu S, Zhu C, et al. Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus. Anal Chim Acta. 2012;723:1–6.Google Scholar
  25. 25.
    Wang F, Banerjee D, Liu Y, et al. Upconversion nanoparticles in biological labeling, imaging, and therapy. Analyst. 2010;135(8):1839–54.Google Scholar
  26. 26.
    De Wild J, Meijerink A, Rath JK, et al. Upconverter solar cells: materials and applications. Energy Environ Sci. 2011;4(12):4835–48.Google Scholar
  27. 27.
    Hu H, Xiong L, Zhou J, et al. Multimodal-luminescence core-shell nanocomposites for targeted imaging of tumor cells. Chem-Eur J. 2009;15(14):3577–84.Google Scholar
  28. 28.
    Kumar R, Nyk M, Ohulchanskyy TY, et al. Combined optical and MR bioimaging using rare earth ion doped NaYF4 nanocrystals. Adv Funct Mater. 2009;19(6):853–9.Google Scholar
  29. 29.
    Zong J, Zhu Y, Yang X, et al. Synthesis of photoluminescent carbogenic dots using mesoporous silica spheres as nanoreactors. Chem Commun. 2011;47(2):764–6.Google Scholar
  30. 30.
    Wang X, Yao Q, Tang X, et al. A highly selective and sensitive colorimetric detection of uric acid in human serum based on MoS2-catalyzed oxidation TMB. Anal Bioanal Chem. 2019;411(4):943–52.Google Scholar
  31. 31.
    Zhu X, Zuo X, Hu R, et al. Hydrothermal synthesis of two photoluminescent nitrogen-doped graphene quantum dots emitted green and khaki luminescence. Mater Chem Phys. 2014;147(3):963–7.Google Scholar
  32. 32.
    Chen G, Qiu H, Prasad PN, et al. Upconversion nanoparticles: design, nanochemistry, and applications in theranostics. Chem Rev. 2014;114(10):5161–214.Google Scholar
  33. 33.
    Qian Y, Yu WJ, Lv CG, et al. Multiphoton upconversion fluorescence properties of heteroaromatic push-pull polymer. Acta Phys-Chim Sin. 2009;25(6):1149–55.Google Scholar
  34. 34.
    Liu CG, Xu YZ, Wei YJ, et al. Spectral properties, protonation and fluorescence quantum yield of ciprofloxacin. Spectrosc Spectr Anal. 2005;25(9):1446–50.Google Scholar
  35. 35.
    Liming D, Qingqin X, Jianmei Y. Fluorescence spectroscopy determination of fluoroquinolones by charge-transfer reaction. J Pharm Biomed Anal. 2003;33(4):693–8.Google Scholar
  36. 36.
    Yang R, Fu Y, Li LD, et al. Medium effects on fluorescence of ciprofloxacin hydrochloride. Spectrochim Acta A. 2003;59(12):2723–32.Google Scholar
  37. 37.
    Wang Y, Yu YH, Yan TX. Study on relationship between quality of life and emotional disorder of patients with rectal cancer during radiotherapy. J Pract Oncol. 2009;24(2):180–3.Google Scholar
  38. 38.
    Zhang R, Liang L, Meng Q, et al. Responsive upconversion nanoprobe for background-free hypochlorous acid detection and bioimaging. Small. 2019;15(2):1803712.Google Scholar
  39. 39.
    Zheng Y, Wang Z, Lui G, et al. Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in micro-volumes of human plasma using high performance liquid chromatography with ultraviolet detection. Biomed Chromatogr. 2019:e4506.Google Scholar
  40. 40.
    Zheng MM, Gong R, Zhao X, et al. Selective sample pretreatment by molecularly imprinted polymer monolith for the analysis of fluoroquinolones from milk samples. J Chromatogr A. 2010;1217(14):2075–81.Google Scholar
  41. 41.
    Silva TIB, Moreira FTC, Truta LA, et al. Novel optical PVC probes for on-site detection/determination of fluoroquinolones in a solid/liquid interface: application to the determination of norfloxacin in aquaculture water. Biosens Bioelectron. 2012;36(1):199–206.Google Scholar
  42. 42.
    Fernández F, Pinacho DG, Gratacós-Cubarsí M, et al. Immunochemical determination of fluoroquinolone antibiotics in cattle hair: a strategy to ensure food safety. Food Chem. 2014;157:221–8.Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Qiuju Zhou
    • 1
  • Xiaoyan Deng
    • 1
  • Yajun Fang
    • 1
  • Kejun Tan
    • 1
    Email author
  1. 1.Key Laboratory of Luminescent and Real-Time Analytical Chemistry, College of Chemistry and Chemical EngineeringSouthwest UniversityChongqingChina

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