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An ultrasensitive electrochemiluminescence immunosensor for alpha-fetoprotein based on a poly(aniline-luminol)/graphene oxide nanocomposite

  • Lu Wei
  • Yanhui Zhang
  • Nurguzal Eziz
  • Yaru Yang
  • Guixin LiEmail author
  • Ming Guan
Research Paper
  • 34 Downloads

Abstract

An ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on a poly(aniline-luminol)/graphene oxide nanocomposite was developed. The nanocomposite, which was prepared using a fast and simple chemical oxidation strategy for the first time, showed excellent ECL performance. This outstanding ECL performance is due to the formation of poly(aniline-luminol) on the graphene oxide (GO) surface and the excellent electron-transfer properties of GO. Moreover, the poly(aniline-luminol)/graphene oxide nanocomposite has abundant amino groups at its surface, making it a good platform for biomacromolecule labeling. Using the nanocomposite, a novel ECL immunosensor for the determination of AFP was successfully developed. Anti-AFP was immobilized on the surface of a poly(aniline-luminol)/graphene oxide nanocomposite-modified electrode using a glutaraldehyde crosslinking method to form the ECL immunosensor. The AFP was then captured at the modified electrode surface through an antigen–antibody immunoreaction. When the AFP was captured by its antibody, the ECL intensity decreased. This ECL immunosensor for the detection of AFP exhibited a linear range of 1.7 × 10−12 to 1.7 × 10−8 mg mL−1 and a detection limit of 5 × 10−13 mg mL−1, indicating high sensitivity and linearity across a wide concentration range. Furthermore, the immunosensor was successfully applied to determine AFP in a real-world human serum sample.

Graphical abstract

A new poly(aniline-luminol)/graphene oxide nanocomposite was prepared using a fast and simple strategy for the first time, and an ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on this nanocomposite was developed

Keywords

Alpha-fetoprotein Electrochemiluminescence Immunosensor Chemical oxidation Poly(aniline-luminol)/graphene oxide nanocomposite 

Notes

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (no. 21665025), the Outstanding Young Science and Technology Personnel Training Project of the Xinjiang Uyghur Autonomous Region (no. QN2016YX0306), and Key Projects of the Engineering Research Center of Electrochemical Technology and Application of Xinjiang Normal Universitly (XJNUGCZX122017A01); all of this support is gratefully acknowledged.

Compliance with ethical standards

Ethics approval and consent to participate

All procedures performed in this study involving human participants were in accordance with the ethical standards of the 1964 Declaration of Helsinki and its later amendments, and were approved by the Ethical Committee of Xinjiang Normal University. All blood samples were from healthy persons, and informed consent was obtained from all individual participants included in the study.

Human and animal rights

No violation of human or animal rights occurred during this investigation.

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

216_2019_1897_MOESM1_ESM.pdf (3 mb)
ESM 1 (PDF 2.96 mb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Lu Wei
    • 1
  • Yanhui Zhang
    • 1
  • Nurguzal Eziz
    • 2
  • Yaru Yang
    • 1
  • Guixin Li
    • 1
    Email author
  • Ming Guan
    • 1
  1. 1.Engineering Research Center of Electrochemical Technology and Application, School of Chemistry and Chemical EngineeringXinjiang Normal UniversityUrumqiChina
  2. 2.School of ScienceHotan Teachers CollegeHotanChina

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