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Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin

  • Andreea Cernat
  • Alexandra Canciu
  • Mihaela Tertis
  • Florin GraurEmail author
  • Cecilia CristeaEmail author
Research Paper

Abstract

The rapid detection of bacterial strains has become a major topic thoroughly discussed across the biomedical field. Paired with the existence of nosocomial pathogen agents that imply extreme medical and financial challenges throughout diagnosis and treatment, the development of rapid and easy-to-use sensing devices has gained an increased amount of attention. Moreover, antibiotic resistance considered by World Health Organization as one of the “biggest threats to global health, food security, and development today” enables this topic as high priority. Pseudomonas aeruginosa, one of the most ubiquitous bacterial strains, has various quorum-sensing systems that are a direct cause of their virulence. One of them is represented by pyocyanin, a blue pigment with electroactive properties that is synthesized from early stages of bacterial colonization. Thus, the sensitive detection of this biomarker could enable a personalized and efficient therapy. It was achieved with the development of an electrochemical sensor based on a thermosensitive polymer, modified with Au/Ag nanoalloy for the rapid and accurate detection of pyocyanin, a virulence biomarker of Pseudomonas aeruginosa. The sensor displayed a linear range from 0.12 to 25 μM, and a limit of detection of 0.04 μM (signal/noise = 3). It was successfully tested in real samples spiked with the target analyte without any pretreatment other than a dilution step. The detection of pyocyanin with high recovery in whole blood in a time frame of 5–10 min from the moment of collection was performed with this electrochemical sensor.

Graphical abstract

Keywords

Pyocyanin Pseudomonas aeruginosa Agar Au/Ag alloy Electrochemical sensor 

Notes

Funding information

This work was supported by grants of the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI-UEFISCDI, project number PN-III-P1-1.2-PCCDI2017-0407 (INTELMAT).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Analytical Chemistry Department, Faculty of PharmacyIuliu Haţieganu University of Medicine and PharmacyCluj-NapocaRomania
  2. 2.Department of Surgery, Faculty of General MedicineIuliu Haţieganu University of Medicine and PharmacyCluj-NapocaRomania
  3. 3.Regional Institute of Gastroenterology and Hepatology “Octavian Fodor”Cluj-NapocaRomania

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