Analytical and Bioanalytical Chemistry

, Volume 411, Issue 16, pp 3631–3640 | Cite as

Polyaniline immobilized on polycaprolactam nanofibers as a sorbent in electrochemically controlled solid-phase microextraction coupled with HPLC for the determination of angiotensin II receptor antagonists in human blood plasma

  • Reyhaneh Esfandiarnejad
  • Hassan SereshtiEmail author
  • Ali Farahani
Research Paper


In this research, electrospun polycaprolactam nanofibers were collected on a fine stainless steel mesh sheet without a binder, and a layer of conductive polyaniline was chemically deposited on the nanofibers. The polyaniline immobilized on the polycaprolactam nanofibers provided high electrical conductivity, acceptable mechanical stability, and a large surface area. This assembly was then used as a working electrode in electrochemically controlled solid-phase microextraction (EC-SPME), a fast and environmentally friendly method. The polymer layers were characterized by SEM and FTIR techniques. Significant factors affecting the EC-SPME efficiency were investigated, including the desorption conditions, the sorbent used, the pH of the sample solution, the extraction voltage, the extraction time, and the ionic strength. Under the optimum conditions, the limits of detection and quantification for the target analytes were 0.9–1.8 μg L−1 and 3.0–6.1 μg L−1, respectively. The linear dynamic range was 5–2000 μg L−1, with R2 > 0.993. The method was coupled with HPLC analysis and applied to the determination of angiotensin ΙΙ receptor antagonists (ARA-ΙΙs) in human plasma, and relative recoveries of 91.1–104.3% with RSDs of ≤8.3% were obtained.


Electrochemically controlled solid-phase extraction Electrospinning Polycaprolactam/polyaniline nanofibers Angiotensin ΙΙ receptor antagonists 


Compliance with ethical standards

The human blood plasma sample was obtained from a healthy volunteer, with informed consent provided to the Iranian Blood Transfusion Organization (Tehran, Iran).

The studies were performed in accordance with the ethical standards approved by the appropriate research ethics committee of University of Tehran.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

216_2019_1845_MOESM1_ESM.pdf (259 kb)
ESM 1 (PDF 259 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Reyhaneh Esfandiarnejad
    • 1
  • Hassan Sereshti
    • 1
    Email author
  • Ali Farahani
    • 1
  1. 1.School of Chemistry, College of ScienceUniversity of TehranTehranIran

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