Characterization of the chemical composition of Drimia numidica plant parts using high-resolution mass spectrometry: study of their total phenolic content and antioxidant activity
Drimia species have been used since ancient times for their medicinal properties. Their bulbs are considered as the main source of secondary metabolites with biological activity but the chemical composition of the other plant parts has not yet been considered. The aim of this study is to contribute to the existing knowledge with new data on the total phenolic content, the antioxidant activity and the chemical profile of different parts of Drimia numidica. The total phenolic content was estimated by the Folin-Ciocalteu assay and the antioxidant activity with DPPH· and ABTS·+ reagents. The separation and the identification of the compounds were performed with liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The extract of leaves presented the highest phenolic content while the highest antioxidant activity was presented by the extract of flowers. Results of the chemical analysis verify the presence of bufadienolides and phenolic compounds.
KeywordsDrimia numidica LC/Q-TOF/HRMS Antioxidant activity Phenolic compounds Bufadienolides
The authors want to thank Dr. Georgios Danezis for his valuable mentoring and support regarding the Folin-Ciocalteu validation experiments.
Compliance with ethical standards
This article does not contain any studies involving animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
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