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Detection of very long-chain hydrocarbons by laser mass spectrometry reveals novel species-, sex-, and age-dependent differences in the cuticular profiles of three Nasonia species

  • Tanja Bien
  • Jürgen GadauEmail author
  • Andreas Schnapp
  • Joanne Y. Yew
  • Christian Sievert
  • Klaus DreisewerdEmail author
Research Paper

Abstract

Long-chain cuticular hydrocarbons (CHC) are key components of chemical communication in many insects. The parasitoid jewel wasps from the genus Nasonia use their CHC profile as sex pheromone and for species recognition. The standard analytical tool to analyze CHC is gas chromatography coupled with mass spectrometric detection (GC/MS). This method reliably identifies short- to long-chain alkanes and alkenes, but CHC with more than 40 carbon atoms are usually not detected. Here, we applied two laser mass spectrometry (MS) techniques, namely direct laser desorption/ionization (d)LDI and silver-assisted (Ag-)LDI MS, respectively, to analyze CHC profiles of N. vitripennis, N. giraulti, and N. longicornis directly from the cuticle or extracts. Furthermore, we applied direct analysis in real-time (DART) MS as another orthogonal technique for extracts. The three methods corroborated previous results based on GC/MS, i.e., the production of CHC with carbon numbers between C25 and C40. However, we discovered a novel series of long-chain CHC ranging from C41 to C51/C52. Additionally, several previously unreported singly and doubly unsaturated alkenes in the C31-C39 range were found. Use of principal component analysis (PCA) revealed that the composition of the newly discovered CHC varies significantly between species, sex, and age of the animals. Our study adds to the growing literature on the presence of very long-chain CHC in insects and hints at putative roles in insect communication.

Graphical abstract

Keywords

Laser mass spectrometry Nasonia Long-chain cuticular hydrocarbons Principal component analysis DART 

Notes

Acknowledgements

We thank Jens Soltwisch for the help with the oTOF mass spectrometer. Financial support by the German Research Foundation (DFG; grant DR 416/10-1 to K.D.), the Department of Defense US Army Research Laboratory (grant W911NF-16-1-0216 to J.Y.Y.), and the University of Münster for travel subsidies within their Internationalisierungsstrategie (to K.D.) is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1736_MOESM1_ESM.pdf (941 kb)
ESM 1 (PDF 941 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Tanja Bien
    • 1
    • 2
  • Jürgen Gadau
    • 3
    Email author
  • Andreas Schnapp
    • 1
  • Joanne Y. Yew
    • 4
  • Christian Sievert
    • 3
  • Klaus Dreisewerd
    • 1
    • 2
    Email author
  1. 1.Institute for HygieneUniversity of MünsterMünsterGermany
  2. 2.Interdisciplinary Center for Clinical Research (IZKF)University of MünsterMünsterGermany
  3. 3.Institute for Evolution and BiodiversityUniversity of MünsterMünsterGermany
  4. 4.Pacific Biosciences Research CenterUniversity of Hawai‘i at MānoaHonoluluUSA

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