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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 12, pp 2697–2705 | Cite as

Screening gut microbial trimethylamine production by fast and cost-effective capillary electrophoresis

  • Virginia García-Cañas
  • Esteban Aznar
  • Carolina SimóEmail author
Research Paper

Abstract

The present work is aimed to develop a simple, rapid, and cost-effective CE method for the determination of trimethylamine (TMA) from bacterial origin. Optimum separation of TMA from the other components of the bacterial culture was achieved using a fused silica capillary (27 cm × 75 μm ID) and a background electrolyte solution that consisted of 0.75 M formic acid at pH 2.05. Analytical characteristics of the proposed method were evaluated through the study of its specificity, linearity, precision, accuracy, robustness, and detection/quantitation limit values. The method was linear over the range 25–2000 μM (R2 = 0.9998). The LOD and LOQ were 9 μM and 27 μM, respectively. Intra-day and inter-day RSD were ≤ 0.24% and ≤ 1.3% for migration time, respectively. Intra-day and inter-day RSD for peak area were ≤ 2.44% and ≤ 3.51%, respectively. The method showed a good accuracy with recovery percentages ranging from 95.45 to 102.21%. The method was successfully applied for the determination of microbial conversion of l-carnitine to TMA. The method shows great potential in high-throughput screening applications to assess the functionality of the gut microbiota to produce TMA.

Graphical abstract

Keywords

Trimethylamine Capillary electrophoresis TMA-producing microorganism Carnitine monooxygenase Gut microbial metabolite 

Abbreviations

BGE

Background electrolyte

FMO

Flavin-containing monooxygenase

TMA

Trimethylamine

TMAO

Trimethylamine N-oxide

Notes

Acknowledgments

The authors wish to thank Prof. Jonas Bergquist from Uppsala University for the donation of the P/ACE 5010 capillary electrophoresis system and Dr. Alberto Valdés for his support during the donation of the equipment. The authors also thank Prof. Rosario Muñoz (ICTAN-CSIC) and Dr. Héctor Rodríguez (CIC bioGUNE) for their kind donation of bacteria strains.

Funding information

This work was funded by the Spanish Ministry of Science, Innovation and Universities (project AGL2017-89055-R).

Compliance with ethical standards

The ethical standards of the respective institutions as directed by the institutional biosafety and ethical committees have been adhered to.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1716_MOESM1_ESM.pdf (519 kb)
ESM 1 (PDF 518 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Virginia García-Cañas
    • 1
  • Esteban Aznar
    • 2
  • Carolina Simó
    • 1
    Email author
  1. 1.Molecular Nutrition and MetabolismInstitute of Food Science Research (CIAL, CSIC)MadridSpain
  2. 2.Área de MicrobiologíaLaboratorio Clínico CentralSan Sebastián de los ReyesSpain

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