The role of incurred materials in method development and validation to account for food processing effects in food allergen analysis

  • Monica MattarozziEmail author
  • Maria Careri
Part of the following topical collections:
  1. Young Investigators in (Bio-)Analytical Chemistry


The issue of undeclared allergens represents a matter of great concern, being the subject of many alert notifications by the Rapid Alert System for Food and Feed portal of the European Commission, often leading to food recalls. The availability of reliable analytical approaches able to detect and quantify hidden allergens in processed foods is increasingly requested by the food industry, food safety authorities and regulatory bodies to protect sensitive consumers’ health. The present review discusses the fundamental role of incurred materials for method development and analytical performance assessment in a metrology perspective on testing for undeclared allergens in processed foodstuffs. Due to the nature of the analytes and their susceptibility to various processing effects, reliability and comparability of results have posed a great challenge. In this context, the use of incurred samples as reference materials permits simulation of the effects of food processing on target analyte structure affecting analyte extractability and detectability.

Graphical abstract


Food allergens Incurred materials Method validation Reference materials Food processing Food safety 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità AmbientaleUniversità di ParmaParmaItaly
  2. 2.Centro Interdipartimentale SITEIA.PARMAUniversità di ParmaParmaItaly

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