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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 8, pp 1647–1657 | Cite as

Multifunctional N,S co-doped carbon dots for sensitive probing of temperature, ferric ion, and methotrexate

  • Pengli ZuoEmail author
  • Jianhua Liu
  • Hongna Guo
  • Chenghong Wang
  • Hongqian Liu
  • Zhigang Zhang
  • Qingyou Liu
Research Paper

Abstract

In this paper, we have presented a facile method to fabricate nitrogen and sulfur co-doped carbon dots (N,S-CDs) for blood methotrexate (MTX) sensing applications. The N,S-CDs with quantum yield up to 75% were obtained by one-step hydrothermal carbonization, using reduced glutathione and citric acid as the precursors. With this approach, the formation and the surface passivation of N,S-CDs were carried out simultaneously, resulting in intrinsic fluorescence emission. Owing to their pronounced temperature dependence of the fluorescence emission spectra, resultant N,S-CDs can work as versatile nanothermometry devices by taking advantage of the temperature sensitivity of their emission intensity. In addition, the obtained N,S-CDs facilitated high selectivity detection of Fe3+ ions with a detection limit as low as 0.31 μM and a wide linear range from 3.33 to 99.90 μM. More importantly, the added MTX selectively led to the fluorescence quenching of the N,S-CDs. Such fluorescence responses were used for well quantifying MTX in the range of 2.93 to 117.40 μM, and the detection limit was down to 0.95 μM. Due to “inert” surface, the N,S-CDs well resisted the interferences from various biomolecules and exhibited excellent selectivity. The proposed sensing system was successfully used for the assay of MTX in human plasma. Due to simplicity, sensitivity, selectivity, and low cost, it exhibits great promise as a practical platform for MTX sensing in biological samples.

Graphical Abstract

Keywords

Doped carbon dots Multifunctional probe Hydrothermal carbonization Excitation-independent emission Surface passivation Methotrexate 

Notes

Funding

This work was supported by the Key Research & Development Program of Shandong Province (2018GGX109006), Independent Innovation Fund Project of Agricultural Science and Technology of Jiangsu Province in 2017 (No.CX (17)1003), and China Scholarship Council.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interests.

Ethical standards and informed consent

Whole blood sample was collected from a healthy volunteer in Linyi Central Hospital and informed consent was obtained for the use of human blood. This research was approved by Linyi Central Hospital Ethic Committee and all experiments were performed in accordance with the Guideline for Experimentation of Linyi Central Hospital.

Supplementary material

216_2019_1617_MOESM1_ESM.pdf (370 kb)
ESM 1 (PDF 369 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Pengli Zuo
    • 1
    • 2
    Email author
  • Jianhua Liu
    • 1
  • Hongna Guo
    • 1
  • Chenghong Wang
    • 1
  • Hongqian Liu
    • 1
  • Zhigang Zhang
    • 1
  • Qingyou Liu
    • 3
  1. 1.Central LaboratoryLinyi Central HospitalLinyiChina
  2. 2.Ilse Katz Institute for NanotechnologyBen Gurion University of the NegevBeer ShevaIsrael
  3. 3.Linyi Center for Disease Prevention and ControlLinyiChina

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