Analytical and Bioanalytical Chemistry

, Volume 411, Issue 7, pp 1453–1465 | Cite as

Simultaneous detection of fumonisin B1 and ochratoxin A using dual-color, time-resolved luminescent nanoparticles (NaYF4: Ce, Tb and NH2-Eu/DPA@SiO2) as labels

  • Sobia Niazi
  • Imran Mahmood Khan
  • Lv Yan
  • Muhammad Issa Khan
  • Ali Mohsin
  • Nuo Duan
  • Shijia Wu
  • Zhouping WangEmail author
Research Paper


A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1) was developed using multi-color, Ln3+-doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF4: Ce, Tb and NH2-Eu/DPA@SiO2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe3O4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb3+ and Eu3+, respectively, were used to measure FB1 (Y = 19,177.1 + (− 12,054.4)x, R2 = 0.9917) and OTA (Y = 4138.8 + (− 11,182.6)x, R2 = 0.9924), respectively. The limits of detection (LODs) for FB1 and OTA were 0.019 pg mL−1 and 0.015 pg mL−1, respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB1 while detection range varied from 0.0001–0.5 ng mL−1. This aptasensor was effectively applied to quantity FB1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.

Graphical abstract


Time-resolved fluorescence nanoparticles Aptamer Mycotoxins Simultaneous detection 


Funding information

This work was partly funded by the National Natural Science Foundation of China (31871881), Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) (CX(18)2025), S&T Support Program of Jiangsu Province (BE2017623), the National First-class Discipline Program of Food Science and Technology (JUFSTR20180303), JUSRP51714B, and the Distinguished Professor Program of Jiangsu Province.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict/competing interest and manuscript is approved by all authors for publication.

Supplementary material

216_2019_1580_MOESM1_ESM.pdf (1.1 mb)
ESM 1 (PDF 1151 kb)


  1. 1.
    Binder E, Tan L, Chin L, Handl J, Richard J. Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Anim Feed Sci Technol. 2007;137(3–4):265–82. Scholar
  2. 2.
    Van Egmond H, Jonker M. Worldwide regulations for mycotoxins in food and feed in 2003. Food and agriculture Organization of the United Nations. 2004.
  3. 3.
    Capriotti AL, Foglia P, Gubbiotti R, Roccia C, Samperi R, Laganà A. Development and validation of a liquid chromatography/atmospheric pressure photoionization-tandem mass spectrometric method for the analysis of mycotoxins subjected to commission regulation (EC) no. 1881/2006 in cereals. J Chromatogr A. 2010;1217(39):6044–51. Scholar
  4. 4.
    Wen J, Mu P, Deng Y. Mycotoxins: cytotoxicity and biotransformation in animal cells. Toxicol Res. 2016;5(2):377–87 Scholar
  5. 5.
    Commission E. Opinion of the scientific committee on food on fusarium toxins part 3: fumonisin B1 (FB1) 2000.
  6. 6.
    Akdemir C, Ulker OC, Basaran A, Ozkaya S, Karakaya A. Estimation of ochratoxin A in some Turkish populations: an analysis in urine as a simple, sensitive and reliable biomarker. Food Chem Toxicol. 2010;48(3):877–82. Scholar
  7. 7.
    Vettorazzi A, Gonzalez-Peñas E, Troconiz I, Arbillaga L, Corcuera L, Gil A, et al. A different kinetic profile of ochratoxin A in mature male rats. Food Chem Toxicol. 2009;47(8):1921–7. Scholar
  8. 8.
    Regulation EC. Setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union. 1881;2006:L364 Scholar
  9. 9.
    Solfrizzo M, Gambacorta L, Lattanzio VM, Powers S, Visconti A. Simultaneous LC–MS/MS determination of aflatoxin M1, ochratoxin A, deoxynivalenol, de-epoxydeoxynivalenol, α and β-zearalenols and fumonisin B1 in urine as a multi-biomarker method to assess exposure to mycotoxins. Anal Bioanal Chem. 2011;401(9):2831. Scholar
  10. 10.
    Wang Y, Chai T, Lu G, Quan C, Duan H, Yao M, et al. Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography. Environ Res. 2008;107(2):139–44. Scholar
  11. 11.
    Yue S, Jie X, Wei L, Bin C, Dou Dou W, Yi Y, et al. Simultaneous detection of ochratoxin A and fumonisin B1 in cereal samples using an aptamer-photonic crystal encoded suspension array. Anal Chem. 2014;86(23):11797–802 Scholar
  12. 12.
    Kim D, Pushkarsky I, Tay A, Di Carlo D. Research highlights: aptamers on a chip. Lab Chip. 2015;15(7):1630–3 Scholar
  13. 13.
    Zhao H, Gao S, Liu M, Chang Y, Fan X, Quan X. Fluorescent assay for oxytetracycline based on a long-chain aptamer assembled onto reduced graphene oxide. Microchim Acta. 2013;180(9–10):829–35. Scholar
  14. 14.
    Fang X, Tan W. Aptamers generated from cell-SELEX for molecular medicine: a chemical biology approach. Acc Chem Res. 2009;43(1):48–57 Scholar
  15. 15.
    Li F, Zhang H, Wang Z, Newbigging AM, Reid MS, Li X-F, et al. Aptamers facilitating amplified detection of biomolecules. Anal Chem. 2014;87(1):274–92 Scholar
  16. 16.
    Xue S-F, Lu L-F, Wang Q-X, Zhang S, Zhang M, Shi G. An integrated logic system for time-resolved fluorescent “turn-on” detection of cysteine and histidine base on terbium (III) coordination polymer–copper (II) ensemble. Talanta. 2016;158:208–13. Scholar
  17. 17.
    Zhu D, Chen Y, Jiang L, Geng J, Zhang J, Zhu J-J. Manganese-doped ZnSe quantum dots as a probe for time-resolved fluorescence detection of 5-fluorouracil. Anal Chem. 2011;83(23):9076–81 Scholar
  18. 18.
    Mader HS, Kele P, Saleh SM, Wolfbeis OS. Upconverting luminescent nanoparticles for use in bioconjugation and bioimaging. Curr Opin Chem Biol. 2010;14(5):582–96. Scholar
  19. 19.
    Rahman P, Green M. The synthesis of rare earth fluoride based nanoparticles. Nanoscale. 2009;1(2):214–24 Scholar
  20. 20.
    Vetrone F, Naccache R, de la Fuente AJ, Sanz-Rodríguez F, Blazquez-Castro A, Rodriguez EM, et al. Intracellular imaging of HeLa cells by non-functionalized NaYF4: Er 3+, Yb 3+ upconverting nanoparticles. Nanoscale. 2010;2(4):495–8 Scholar
  21. 21.
    Vetrone F, Naccache R, Mahalingam V, Morgan CG, Capobianco JA. The active-core/active-shell approach: a strategy to enhance the upconversion luminescence in lanthanide-doped nanoparticles. Adv Funct Mater. 2009;19(18):2924–9. Scholar
  22. 22.
    Urusov AE, Petrakova AV, Vozniak MV, Zherdev AV, Dzantiev BB. Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles. Sensors. 2014;14(11):21843–57 Scholar
  23. 23.
    Brandão D, Liébana S, Pividori MI. Multiplexed detection of foodborne pathogens based on magnetic particles. New Biotechnol. 2015;32(5):511–20. Scholar
  24. 24.
    Joshi R, Janagama H, Dwivedi HP, Kumar TS, Jaykus L-A, Schefers J, et al. Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars. Mol Cell Probes. 2009;23(1):20–8. Scholar
  25. 25.
    McKeague M, Bradley CR, Girolamo AD, Visconti A, Miller JD, DeRosa MC. Screening and initial binding assessment of fumonisin B1 aptamers. Int J Mol Sci. 2010;11(12):4864–81 Scholar
  26. 26.
    Cruz-Aguado JA, Penner G. Determination of ochratoxin A with a DNA aptamer. J Agric Food Chem. 2008;56(22):10456–61 Scholar
  27. 27.
    Wang L, Bao J, Wang L, Zhang F, Li Y. One-pot synthesis and bioapplication of amine-functionalized magnetite nanoparticles and hollow nanospheres. Chem Eur J. 2006;12(24):6341–7. Scholar
  28. 28.
    Tu D, Liu L, Ju Q, Liu Y, Zhu H, Li R, et al. Time-resolved FRET biosensor based on amine-functionalized lanthanide-doped NaYF4 nanocrystals. Angew Chem Int Ed. 2011;50:6306–10. Scholar
  29. 29.
    Tan H, Li Q, Ma C, Song Y, Xu F, Chen S, et al. Lanthanide based dual-emission fluorescent probe for detection of mercury (II) in milk. Biosens Bioelectron. 2015;63:566–71. Scholar
  30. 30.
    Huang Y, Zhang H, Chen X, Wang X, Duan N, Wu S, et al. A multicolor time-resolved fluorescence aptasensor for the simultaneous detection of multiplex Staphylococcus aureus enterotoxins in the milk. Biosens Bioelectron. 2015;74:170–6. Scholar
  31. 31.
    Wu S, Duan N, Zhu C, Ma X, Wang M, Wang Z. Magnetic nanobead-based immunoassay for the simultaneous detection of aflatoxin B1 and ochratoxin a using upconversion nanoparticles as multicolor labels. Biosens Bioelectron. 2011;30(1):35–42. Scholar
  32. 32.
    Wang Y-K, Yan Y-X, Ji W-H, Wang H-A, Li S-Q, Zou Q, et al. Rapid simultaneous quantification of zearalenone and fumonisin B1 in corn and wheat by lateral flow dual immunoassay. J Agric Food Chem. 2013;61:5031–6 Scholar
  33. 33.
    Ganbold E-O, Lee CM, Cho E-M, Son SJ, Kim S, Joo S-W, et al. Subnanomolar detection of ochratoxin A using aptamer-attached silver nanoparticles and surface-enhanced Raman scattering. Anal Methods. 2014;6:3573–7 Scholar
  34. 34.
    Bian Y, Huang X, Ren J. Sensitive and homogenous immunoassay of fumonisin in foods using single molecule fluorescence correlation spectroscopy. Anal Methods. 2016;8:1333–8 Scholar
  35. 35.
    Rauová D. Single laboratory-validated HPLC methods for determination of ochratoxin A, fumonisin B1 and B2, zearalenone and deoxynivalenol in cereals and cereal-based foods. J Food Nutr Res. 2010;49:57–68 Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Sobia Niazi
    • 1
    • 2
    • 3
    • 4
  • Imran Mahmood Khan
    • 1
    • 2
    • 3
    • 4
  • Lv Yan
    • 1
    • 2
    • 3
    • 4
  • Muhammad Issa Khan
    • 5
  • Ali Mohsin
    • 6
  • Nuo Duan
    • 1
    • 2
    • 3
    • 4
  • Shijia Wu
    • 1
    • 2
    • 3
    • 4
  • Zhouping Wang
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.School of Food Science and TechnologyJiangnan UniversityWuxiChina
  3. 3.International Joint Laboratory on Food SafetyJiangnan UniversityWuxiChina
  4. 4.Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu ProvinceJiangnan UniversityWuxiChina
  5. 5.National Institute of Food Science and TechnologyUniversity of AgricultureFaisalabadPakistan
  6. 6.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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