Analytical and Bioanalytical Chemistry

, Volume 411, Issue 6, pp 1169–1180 | Cite as

Utilization of atmospheric solids analysis probe mass spectrometry for analysis of fatty acids on seed surface

  • Monika Cechová
  • Iveta Hradilová
  • Petr Smýkal
  • Petr Barták
  • Petr BednářEmail author
Research Paper


Atmospheric solids analysis probe mass spectrometry (ASAP-MS) was used for the first time for direct surface analysis of plant material. It can be readily used for surface analysis of whole and intact pea seeds and their seed coats, and for the study of the profile of fatty acids on the outer surface. Furthermore, ASAP-MS in combination with multivariate statistics allowed classification of pea genotypes with respect to physical dormancy and investigation of related biological markers. Hexacosanoic and octacosanoic acids were suggested to be important markers likely influencing water transport through the seed coat into the embryo (with the highest significance for dormant L100 genotype). ASAP-MS provided higher selectivity and better signal of fatty acids compared to (MA)LDI-MS (laser desorption ionization mass spectrometry either matrix free or matrix assisted) providing on the other hand spatial distribution information and results obtained by both methods are mutually supportive. The developed ASAP-MS method and obtained results can be widely utilized in biological, food, and agricultural research.

Graphical abstract


Atmospheric solids analysis probe Mass spectrometry Fatty acid Pea Physical dormancy Legume seed 


Funding information

This study received the support from Operational Programme Research, Development and Education – European Regional Development Fund, project no. CZ.02.1.01/0.0/0.0/16_019/0000754 and Palacký University Olomouc (IGA_PrF_2018_027).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1551_MOESM1_ESM.pdf (1.8 mb)
ESM 1 (PDF 1866 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Monika Cechová
    • 1
  • Iveta Hradilová
    • 2
  • Petr Smýkal
    • 2
  • Petr Barták
    • 1
  • Petr Bednář
    • 1
    Email author
  1. 1.Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of SciencePalacký UniversityOlomoucCzech Republic
  2. 2.Department of Botany, Faculty of SciencePalacký UniversityOlomoucCzech Republic

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